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Articles by M. Das
Total Records ( 4 ) for M. Das
  M. Das , D.K. Deka , P.C. Sarmah , S. Islam and R. Laha
  To determine the prevalence of Giardia duodenalis infection in dairy cattle present in Assam, India, a total of 1176 numbers of faecal samples of cattle comprising 535 numbers of faecal samples of calves and 641 numbers of faecal samples of heifers were screened for one year using Formol-Ether technique and zinc sulphate (33%) floatation method. The overall prevalence of infection in cattle was 17.94%. Age-wise infection rate was 13.45 and 21.68% in calves and heifers, respectively. Season-wise highest infection was recorded during monsoon (30.90%) followed by pre-monsoon (21.97%), post-monsoon (5.50%) and winter (4.45%). Infection was higher among the diarrhoeic (83.41%) than non-diarrhoeic (16.58%) animals. The prevalence of G. duodenalis infection in cattle of Assam has significance because it infects a wide range of hosts, including humans worldwide and responsible for causing diarrhoea.
  M. Das and P. Ramanujam
  Microspora quadrata Hazen, a green filamentous alga has been found to grow luxuriantly throughout the year, in the streams affected by Acid Mine Drainage (AMD) caused by unscientific coal mining in Jaintia Hills, Meghalaya. The AMD effluents contain metals which are known to be toxic to the aquatic organisms. Filamentous green algae are capable of accumulating and thereby help in removing metals from the affected water bodies. The present study was undertaken to find the seasonal variations in the metal content in the AMD impacted streams and the efficiency of Microspora quadrata to remove the toxic metals by accumulation of the metals in its biomass. Water and filamentous algal mats were collected seasonally from streams located in active mining, coal storage and abandoned areas and were analyzed for iron, lead, zinc and manganese by atomic absorption spectrophotometer. Significant seasonal changes in concentrations of toxic metals were observed in water and in Microspora quadrata Hazen. Accumulation of metals like iron and lead were higher in algal mat compared to water medium in all seasons. Maximum accumulation of iron was observed in autumn whereas lead and manganese were more in spring. Zinc content in water was significantly higher than that of algal mat indicating very low rate of absorption or adsorption. Thus, from the present study Microspora quadrata could be suggested as a useful plant material to remove toxic metals like iron lead and manganese from the AMD streams of the region.
  Mst. M. Begum , M.S. Rahman , R.R. Swarna , M. Das , A.H.M.R. Imon , I. Jahan , M. Rahman , Md. E. Haque , R.R. Saha , A.H.M. Quamruzzaman , Md. A. Obaida , M. Maniruzzaman , A. Islam , Md. T. Islam and A. Sarker
  Background and Objective: Combination of dosages regimen of an antidiabetic agent (Glibenclamide) with a lipid lowering drug can be an effective medication for the patient with high blood glucose level and liver enzyme disfunctionality. The present study was undertaken to investigate the effect of a fixed dose combination of glibenclamide (1.2 mg/70 kg b.wt.) and simvastatin (10 mg/70 kg b.wt.) on blood glucose and liver enzymes dysfuntionality in alloxan-induced diabetic rats for an extended time period. Materials and Methods: Two protocols were developed to carry out the experiment. The first is designated as 4 weeks short-term and second one is termed as 12 weeks long-term treatment protocols, respectively. Diabetes Mellitus (DM) was induced by single intraperitoneal (i.p.) injection of freshly prepared alloxan solution in 0.9% saline. Diabetic rats received treatment with i.p., injection of glibenclamide (1.2 mg/70 kg b.wt.) and simvastatin (10 mg/70 kg b.wt.) for 4 weeks as monotherapy and combination therapy (glibenclamide 0.6 mg/70 kg b.wt., simvastatin 5 mg/70 kg b.wt.) for 12 weeks. Graph pad was used and the results were expressed as Mean±SEM. A one-way analysis of variance (ANOVA) followed by Dunnett’s post hoc test or students paired or unpaired t-test was used in the study where appropriate. Results: Results were considered to be significant when p-values were less than 0.05 (p<0.05). Combination therapy demonstrated a significant (p<0.05) decrease in blood glucose and liver enzymes elevation compared with diabetic control group. The study also demonstrated that the short term treatment has satisfactory effect on lowering SGPT by 41% and SGOT by 50%. Long term administration of combination therapy showed more significant (p<0.05) potentiality on lowering SGPT (46%) and SGOT (53%), respectively and this level remain steady during total treatment period. Conclusion: The present study demonstrates that combination of glibenclamide with simvastatin at the dose level tested exhibits significant glucose and liver enzymes lowering activity in alloxan induced diabetic rats. When monotherapy with oral antidiabetic agents fails, combination therapy with glibenclamide plus simvastatin seems to be stable and effective for the treatment of diabetes mellitus.
  S. Pal , H. Rashid , M.A.K. Tarafder , N.T. Narejo and M. Das
  In view of domestication of the endangered species Nandus nandus, the artificial breeding of the fish with carp pituitary gland (PG) was trialed. The optimal dose of PG was found to be 150 mg kg–1 of fish body weight. Best fertilization rate (92±5%) was obtained at 175 and 200 mg kg–1 body weight PG application and best hatching rate (90±2, 90±5, 90±5%) was revealed at 150, 175 and 200 mg kg–1 body weight PG application. Fertilization and hatching rate were 92 and 81% respectively in case of the experiment where only females were injected. Ovulation occurred after 10 to 14 h of injection and the eggs hatched out after 18 to 20 h of fertilization. The larvae started feeding after 56 h of hatching and best survival rate (16%) was achieved by supplying zooplankton as food. It was also found that larvae denied egg yolk and Tubifex worm as their first food.
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