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Articles by M. Ashfaque
Total Records ( 4 ) for M. Ashfaque
  A. Pandey , M. Kamle , L.P. Yadava , M. Muthukumar , P. Kumar , V. Gupta , M. Ashfaque and B.K. Pandey
  In the context of the GM food regulations crop improvement via transgenic technology is a new stage of introducing novel food which supercedes over the conventional breeding. It was analyzed that worlds hunger, malnutrition problems, environmental pollution and phytoremediation in agriculture are the challenges for scientist as well as governments those can be combated by application of genetic engineering in crops. Genetically modified microbes/plant/animals or GM microbes/plant/animals results from modification in the genetic make-up of microorganisms, plants and animals using recombinant DNA technology to improve the nutritional requirement, disease resistant traits, increased production and medicinal properties. In many instances, these modification processes represent faster, more efficient mechanisms for achieving changes than traditional breeding. However, a wide variety of modifications are possible through genetic manipulation and the potential for the introduction of toxic compounds, unexpected secondary effects and changes in nutritional and toxicological characteristics may give rise to safety concerns about GM crops. Thus, generation of GM food explores new vistas for future food requirement but the assessment of policy regarding environmental risks is also to be concerned.
  A. Pandey , M. Kamle , L.P. Yadava , M. Muthukumar , P. Kumar , V. Gupta , M. Ashfaque and B.K. Pandey
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  Masood Akhtar , C.S. Hayat , M. Ashfaque , I. Hussain , Murad Ali Khan and Sultan Ayaz
  Delayed hypersensitivity reaction was used to measure the cell mediated immunity against avian coccidiosis. There was a gradual increase in the comb thickness of the immunized birds of all the groups from 24 to 72 hours after intradermal injection. The mean difference in comb thickness before and after injection of the respective antigen for the group immunized with Vaccine I (sonicated supernatant) was 1.87 mm at 24 hours, 2.23 mm at 36 hours, 2.31 mm at 48 hours and 2.42 mm at 72 hours. There was a significant difference (p<0.01) in comb thickness at 24 and 36 hours after intradermal injection. The mean difference in comb thickness before and after injection of the respective antigen for the group immunized with Vaccine II (sonicated sediment) was 1.12 mm at 24 hours, 1.75 mm at 36 hours, 2.10 mm at 48 hours and 2.31 mm at 72 hours. The difference was significant (p<0.01) at 24 and 48 hours; 36 and 72 hours after injection. Maximum difference in comb thickness was recorded 72 hours after injection in birds immunized with Vaccine I. It appears that delayed hypersensitivity response to sonicated supernatant antigen reported herein represent a strong CMI reaction. Delayed hypersensitivity comb reaction is a quick, simple, economical and practical tool to routinely determine the immune status of a bird with out restoring to challenge.
  Masood Akhtar , Rehan Ahmed , C. S. Hayat , I. Hussain and M. Ashfaque
  Immune response of formalin inactivated and binnary ethyleneimine inactivated Angara disease (hydropericardium) vaccines were compared using indirect hemagglutination (IHA) test. A four fold increase in IHA antibody titre was recorded with binnary ethyleneimine (BEI) inactivated vaccine. Agar gel diffusion test gave a strong precipitation line with serum of chicks vaccinated with BEI inactivated vaccine whereas a weak precipitation line was observed with serum from chicks vaccinated with formalin inactivated vaccine. The results of challenge test were promising and no adverse effects were seen in vaccinated chicks. BEI as inactivating agent produced antigenically superior vaccine.
 
 
 
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