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| Articles
by
M. Anisuzzaman |
Total Records (
3 ) for
M. Anisuzzaman |
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M. Anisuzzaman
,
S. Jarin
,
K. Naher
,
M.M. Akhtar
,
M.J. Alam
,
M. Khalekuzzaman
,
I. Alam
and
M.F. Alam
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A viable protocol has been developed for indirect shoot
organogenesis of okra. To establish a stable and high-frequency plant
regeneration system, leaf disc and hypocotyl explants were tested with
different combinations of α-naphthalene acetic acid (NAA), indole-3-acetic
acid (IAA),iIndole-3-butyric acid (IBA), thidiazuron (TDZ) and 6-benzylaminopurine
(BAP). Morphogenic callus induction was observed in highest frequency
from hypocotyl explant by culturing in MS medium supplemented with 2.0
mg L-1 NAA plus 0.5 mg L-1 TDZ. The highest percentage
of shoot regeneration and highest mean number of shoot per callus mass
was obtained with 2.0 mg L-1 BAP plus 0.1 mg L-1
IBA. Root formation was observed from callus induced in medium containing
1.5 mg L-1 NAA. Morphogenic difference due to explant type
is clear for the studied in vitro traits. About 80% of regenerated
plantlets were survived and showed new leaves development under ex
vitro condition. This protocol would be useful to create somaclonal
variation and to utilize transgenic approach for varietal improvement
of okra. |
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M. Anisuzzaman
,
S.A. Sharmin
,
S.C. Mondal
,
R. Sultana
,
M. Khalekuzzaman
,
I. Alam
and
M.F. Alam
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In vitro microrhizome was successfully produced in Curcuma
zedoaria-a valuable but critically threatened medicinal plant. Ten to twelve
weeks old in vitro multiplied shoots were cultured in MS medium supplemented
with different level of BAP and NAA. Effect of different carbon sources has also
been evaluated. Microrhizome formation started within 7-9 weeks of culture. The
best result was obtained in 4.0 mg L-1
BAP along with 6% sucrose in terms of frequency and number of rhizome. Around
4 microrhizomes with an average weight of 2.5 g were harvested after 11-12 weeks.
Microrhizomes were readily germinated in the growth regulator free MS medium.
With the best of our knowledge this is the first report on in vitro microrhizome
production in Curcuma zedoaria. |
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N.A. Siddique
,
Jaime A. Teixeira da Silva
,
M. Anisuzzaman
and
M.A. Bari
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A procedure for rapid in vitro propagation of the aromatic and medicinal plants Aristolochia indica Linn and Hemidesmus indicus R.B (Family: Aristolochiaceae and Asclepiadaceae respectively) from axillary shoots is described. The highest percentage of callus induction was 92% on MS medium supplemented with 1.0 mg L-1 α-naphthalene acetic acid (NAA) and 1.0 mg L-1 6-benzyladenine (BA) for A. indica while 98% was achieved on MS medium supplemented with 0.5 mg L-1 NAA and 2.0 mg L-1 Kinetin (Kn) for H. indicus. The colour of the calli was mostly light to dark green. Development of adventitious shoots occurred when calli were subcultured on MS medium supplemented with BA and Kn alone or in BA combined with NAA and IAA, or NAA, IAA and BAP combined with Kn. The highest percentage (90%) of shoot regeneration in A. indica was obtained on MS medium fortified with 1.0 mg L-1 BA and 2.5 mg L-1 NAA, but a 98% rate on MS medium supplemented with 2.5 mg L-1 Kn and 1.0 mg L-1 NAA for H. indicus. Regenerated shoots rooted best on MS medium containing 2.5 mg L-1 Kn and 2.0 mg L-1 IBA. Plantlets were transferred to pots containing sand and soil mixture and acclimatized in a culture room. Finally rooted plants were transferred to soil. |
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