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Articles by M. Amzad Hossain
Total Records ( 2 ) for M. Amzad Hossain
  M. Amzad Hossain and A. Rahman
  The chemical composition of the methanolic crude extract of the leaves of cabbage samples collected from the local market in Dhaka Metropolitan City (DMC), Dhaka, Bangladesh was analysed by GC-MS. It was determined that 44 compounds, which represented 66% of organic compounds, were present in the methanolic crude extract. The methanolic crude extract contained the major compounds mainly phenanthrene, anthracene, oxalic acid, napthalene, β-pinene, 2-octen-3-ol, 3-octamol and 3,4-dihydroxymendelic acid. Thus, the extract was found different types of compounds such as caffice acid and mono, di and tri terpenes and their respective hydrocarbons. Methanol extract of cabbage samples and the derived fractions of hexane, chloroform and ethyl acetate were tested for anti-fungal activity, which was determined by disc diffusion and Minimum Inhibitory Concentration (MIC) determination methods. The methanol extract and the derived fractions of methanol showed great potential of anti-fungal activity as a mycelial growth inhibition against the tested some fungi such as Sclerotium rolfsii, Rhizoctonia solani, Aspergillus niger and Aspergillus fumigatus in the range of 54.6-68.0% and minimum inhibitory concentration ranging from 500-1000 μg mL-1.
  M. Amzad Hossain and Zhari Ismail
  The present study was carried out aiming to isolate the flavonoidal components of the plant and test them cytotoxicity. The leaves were extracted with methanol and than fractionated with hexane, chloroform, ethyl acetate and finally with butanol. The crude extracts were purified by using different chromatographic techniques. Two prenylated flavonoid derivatives; 5,7,3',5'-tetramethoxy-8-C-prenyl-flavone, 5,7,3',5'-tetramethoxy-6-C-prenylflavone, together with four known flavonoids 5,6,7,3',4'-pentamethoxyflavone,5-hydroxy-6,7,3',4'-tetramethoxyflavone, ladanein and 6-hydroxy-5,7,4'-trimethoxyflavone isolated from the leaves extracts of Orthosiphon stamineus. The structures of these compounds have been established by spectroscopic methods. All the isolated compounds were tested for their cytotoxicity towards highly liver metasttatic murine colon 26-L5 carcinoma cells and the new prenylated flavones and flavonoids (5,6,7,3',4'-pentamethoxyflavone, 5-hydroxy-6,7,3',4'-tetramethoxy lavone, ladanein and 6-hydroxy-5,7,4'-trimethoxyflavone) showed cytotoxicity with an ED50 value between 10-90 μg mL-1.
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