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| Articles
by
M. Almeslemani |
Total Records (
1 ) for
M. Almeslemani |
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A. Abdul Kader
,
M. Almeslemani
,
A. Baghdady
,
H. Alzubi
,
N. Alasaad
,
N. Ali Basha
,
A. Dameriha
,
H.J. Jacobsen
and
F. Hassan
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hva1, a barley (Hordeum vulgare L.) group III
Late Embryogenesis Abundant (LEA) protein, is highly induced by various stresses
and has successfully been used to confer stable tolerance to abiotic stresses
such as drought and salinity in several transgenic plant species. In the present
study, the hva1 gene from some Syrian barley varieties was isolated,
characterized and then cloned into the monocot.-specific binary vector pAHC25
to enable transformation of crops for drought tolerance. The 3-day-old seedlings
of several Syrian barley varieties were subjected to different stress treatments
(Abscisic acid, (ABA) drought, salt and cold) for gene expression studies. Complementary
DNA (cDNA) was synthesized from isolated RNA using hva1 homologous primers
for amplification of 788 bp. The eluted DNA band was analysed and sequenced,
the obtained sequence was blast using N-blast of NCBI. The results showed similarities
between 80 and 98% to accession number: X78205. The coding region of 642 bp
was cloned resulting in pAHC25-ubi1-hva1-nos construct. This construct
was then transferred into two Agrobacterium strains, i.e., LBA4404 and
EHA105 for transgenic crop improvement mainly to target improved stress tolerance
in crops such as wheat, cotton and maize. |
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