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Articles by M. Afsharnasab
Total Records ( 4 ) for M. Afsharnasab
  M. Afsharnasab , R. Mortezaei , V. Yegane and B. Kazemi
  The importation of Litopenaeus vannamei to Iran from Hawaii was initiated when Iranian shrimp culture was first affected by WSSV in 2004. The main reason for the importation of L. vannamei to Iran was the disease susceptibility and mass mortality of the indigenous species (P. indicus) when faced with the first outbreak of WSSV. During the two years of study, it was found out that culturists in Iran preferred cultured L. vannamei than the local species (P. indicus). In 2008, mass mortality occurred in farmed L. vannamei in Khuzestan Province South of Iran. Two hundred shrimps with white spot on the carapace and body were collected and preserved in Davidson fixative for histopathology. A part of samples collected were also preserved in 95% ethyl alcohol for Polymerase Chain Reaction (PCR) technique. Two pair primers from VP24 WSSV genome was identified and used for PCR while identified one pair primer for 18SrRNA gene of shrimp was used as house keeping gene in PCR reaction in both positive and negative PCR reaction. Grossly, the samples showed white spot in the cuticle and body surface and red color on the appendages. Histopathologically, all tissue except hepatopancreas showed the intranuclear Cowdry type-A inclusion bodies. PCR studies using designated primer revealed a band of 414 bp from WSSV and 809 bp of shrimp DNA fragments in positive samples. The negative samples showed just 809 bp. This is the first report of White Spot Syndrome Virus (WSSV) in farmed L. vannamei in Iran.
  M.K. Pazir , M. Afsharnasab , N. Niamaymandi , H. Khadem , E. Akbarpour and A.A. Zendebudi
  This study was conducted to identify White Spot Syndrome Virus (WSSV) and Infections Hypodermal and Hematopoietic Necrosis Virus (IHHNV) by using clinical and histopathological sings of cultured shrimp, Litopenaeus vannamei in Bushehr farms, from December 2009 to April 2010. Samples were collected from Bushehr shrimp farms and hatcheries. Based on histopathological and gross signs, two viral diseases, WSSV and IHHNV have been detected. The gross signs of WSSV in the moribund samples showed reduced feeding, lethargy, difference in size, reddish coloration of appendages and white plaque on the carapace, while the gross sign of IHHNV exhibited abdominal dorsal changes of the muscles, opaque or milky spot on the cuticles, rostrum deformity syndrome and abdominal segmental abnormalities. The histopathological observations of WSSV showed basophilic Cowdry type A inclusion bodies in all tissues such as gills, haematopoietic tissue, cuticle epithelium, lymphoid organ and connective tissue. However, histologically, the hepatopancreas tissue showed vacuolization of B cells, without inclusion bodies. The histopathological signs observed such as hypertrophy, cellular degeneration and eosinophilic Cowdry type A inclusion bodies in the cells nucleus hepatopancreas, gills, haematopoietic, cuticle epithelium, digestive epithelium, lymphoid organ and connective tissue. Also, histopathological change of the striated muscles of the affected abdominal segmental abnormality showed severe Zenker's necrosis.
  M. Heidarieh , M. Afsharnasab , M. Soltani , A. Dashtyannasab , S. Rajabifar , N. Sheikhzadeh and A.H. Tamimi
  During the period of culture, immunostimulants and even vaccines may provide potential methods to protect shrimps from opportunistic and pathogenic agents. This study was designed to show the effects of Ergosan and Vibromax vaccine against WSSV and Vibrio harveyii in juvenile Litopeaneus vannamei and even growth and survival rate in PL stage. Among different treatments in PL stage, Ergosan significantly enhanced the body weight while in group treated with Ergosan and Vibromax the enhancement in survival rate was observed. Survival rate was also assessed by challenging with Vibrio harveyii and WSSV in juvenile stage. All treatments with Ergosan and Vibromax vaccine showed significant enhancement in survival rate compared to the control groups. This study indicates that Ergosan and Vibromax could be used to promote the health status of shrimp during the period of culture.
  A.M. Saberi , M. Bandehpour , M. Afsharnasab , E. Ghayour , S.A. Yousefi Namin and B. Kazemi
  The aim of this study is designing a diagnostic kit for white spot syndrome virus. We designed 2 series of primers for diagnosis of viral VP24 gene and also primers as internal controls which amplify part of genome in both positive and negative samples. DNA of shrimps were extracted and PCR amplification carried out. In this research, a diagnosis kit for white spot disease of shrimps designed and tested using 32 shrimp samples which were dubious to have this disease. White spot virus were found in 23 samples and the other 9 were negative. For extra confirmation, the PCR product was sequenced and deposited to GenBank. We designed a diagnosis kit for white spot disease of shrimps and tested successfully.
 
 
 
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