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Articles by M. Yasin Ashraf
Total Records ( 8 ) for M. Yasin Ashraf
  Raza Hafeez Ahmad , Muhammad Naeem , M. Yasin Ashraf and Ejaz Rasool
  The research work reported here was carried out to evaluate the effect of salinity and nitrogen on two gram varieties (Punjab-91 and C-44 in pots with three salinity levels 3.5 (control), 7.5 and 11.5 dS m-1 and four nitrogen levels i.e., 60, 75 and 90kg N ha-1 as urea It was observed that plant height increased as the amount of urea increased under 3.5 dS m-1. At higher salinity levels plant height showed retrogressive effect. Number of pod/plant were the maximum when 60 and 75 kg ha-1 urea was applied. But pod plant decreased at higher salinity levels sharply. Biomass of plant also increased with the addition of urea. Number of seeds/ pod were maximum at 3.5 dS m-1 with 60 and 75 kg N ha-1 urea. Number of seeds pod, yield/ plant and 100 seed weight were the maximum when 60 kg N ha-1 urea was added in saline soils. As for as amount of K+ and Na+ are concerned the maximum amount of K+ was observed in leaves while it was minimum in shoots. Urea decreased K+ and Na+ ion uptake in saline soils. Nitrogen percentage in seeds also increased with the addition of urea. But it decreased when 90 kg N ha-1 urea was added in each salinity level.
  Sara Zafar , M. Yasin Ashraf , G. Sarwar , Saqba Mahmood , Abida Kausar and Iftikhar Ali
  Pot cultured experiment consisting of two rice cultivars Basmati-370 (Salt sensitive) and IR6 (salt tolerant) with five replicates each having two different salinity levels was conducted at wire house of Soil Biology Division, NIAB, Faisalabad using randomized complete block design. Growth and chemical analyses of plant samples were carried out at different harvest levels. Results indicated that increasing levels of salinity furnished proportional relationship with increase in Na+ and Cl¯ content with decrease in growth of Basmati-370 whereas K, Ca, N and P content were highly significant in IR-6.
  Saqba Mahmood , Sara Zafar , M. Yasin Ashraf , G. Sarwar , M. Ashraf and M. Naeem
  Biochemical changes in resistant and susceptible cotton cultivars to leaf curl virus (CLCuV) were assessed by a series of laboratory experiments. The experiments were conducted in petri-dishes containing filter papers with sterile distilled water on which seeds of the cotton cultivars i.e. CIM-446, resistant and S-12 susceptible to CLCuV were sown, each cultivar have five replications and petri-dishes were kept in growth cabinet at 28± 2°C. The results showed that the growth attributes proved S-12 superior to CIM-446. But with respect to the biochemical attributes both the cultivars appear to use different biochemical attributes for their germination demands. Soluble sugars translocations and its concentrations were higher in CIM-446 than S-12. In all the three embryonic organs of germinating seeds, starch mobilization had also the same pattern of varietal difference, where CIM-446 had greater ratio of starch, more active enzyme (α-amylase), degrading starch and higher comparative ratio of the resultant product of starch degradation. This may provide better fulfillment of structural requirements to resist virus at vegetative growth stages by contributing readily available energy by active break down of starch and translocation of sugars which may play some role in the composition of antibodies or some other biochemical/physiological responses associated with starch and sugars.
  Sara Zafar , M. Yasin Ashraf and M. Ashraf
  Salinity (NaCl) induced changes in protease activities and associated metabolic changes in three cotton varieties (NIAB-Karishma, NIAB-86 and K-115) were studied during germination and early seedling stages under controlled conditions. The increase in salinity of the medium resulted in the decrease of protease activities in all varieties, however it was more pronounced in NIAB-86. Decrease in mobilization of reserve protein and reduced concentrations of total free amino acids with increasing salinity were recorded in all the cotton varieties. Variety K-115 showed better performance than others. It showed highest germination followed by NIAB-Karishma and NIAB-86. The variety K-115 also had higher to mobilization capacity and had higher levels of total free amino acids and less reserve protein during germination and early seedling growth stages.
  Muhammad Hussain , Muhammad Naeem , M. Yasin Ashraf and Zafar Iqbal
  Effect of salinity on yield of three barley cultivars Jou-83, Jou-87 and Haider-93 were investigated. It was noted that the number of fertile tillers, spike length, 100 seed weight, yield per plant were higher in Jou-83 followed by Jou-87 and Haider-93. Jou-87 produced highest number of grain per ear followed by Jou-83 and Haider-93. Grain yield production was highest in Jou-83. Reduction in yield per plant in highest salinity level compared to control was 77.22 % in Jou-83 while the corresponding value was 91.78 % for Jou-87 and 86.35 % for Haider-93. Accumulation of Na+ and Cl- ions increased with increase in salinity levels in all plant parts (root, stem and leaf). Jou-83 maintained lowest Cl- ion concentration than other varieties while Na+ ion accumulation was slightly higher than Jou-87.
  Muhammad Naeem , Michael J. Emes and M. Yasin Ashraf
  Starch, the major source of calorific intake in the human diet, is synthesised in the amyloplasts of non-photosynthetic tissues. To understand its synthesis, to determine which substrate is taken up by the organelle and which metabolite is broken down for the release of energy, the present studies were started from potato tuber (cv. Record). Glucose 1-phosphate (Glc1P) was the most effective substrate for starch synthesis in amyloplasts. The rate of incorporation of hexose (Glc1P) into starch was dependent on the presence of exogenous ATP and on the intactness of the preparation. Rates of starch synthesis from Glc1P plus ATP were linear for up to 1h and was saturated by 5-10 mM Glc1P. The uptake of Glc1P was inhibited by the addition of exogenous glucose 6-phosphate (Glc6P) and was not effected by the addition of 3-phosphoglyceric acid (3PGA). Other than Glc1P, ADPglucose (ADPG) also supported similar rates of starch synthesis, however, rate was saturated by 1mM ADPG. The import of ADPG and ATP into the amyloplast indirectly indicate the presence of an adenylate translocator on the organelle membrane. Carbohydrate oxidation in the amyloplast was stimulated by the addition of 2-oxoglutarate and glutamine. Glucose 1-phosphate also proved to be a better substrate for oxidative pathways than glucose 6-phosphate. The implications of these results for our understanding of the pathway of starch synthesis and carbohydrate oxidation are discussed.
  M. Naeem , Salah-ud-Din Baber , M. Yasin Ashraf and A.R. Rao
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  Yousaf Ali , M. Yasin Ashraf , Z. Aslam and S.S. Alam
  An experiment was conducted at three different locations of Punjab with twenty-four genotypes of cowpea under natural field conditions where yellow mosaic virus disease is a serious problem. Yellow mosaic virus infestation was rated after 4-5 weeks of sowing. Results indicated that genotypes 1T-95k-1156-3, 1T-94k-137-6, 1T-97k-9042-8,1T-97k-499-4, 1T-97k-497-2, 1T-93k-452,1T-97-k-350-4, SA Dandy, p-518, Elite, No.44 and 1T-84-552 showed highly resistant to resistant reaction, 1T-94k-440-3, 1T-95k-627-34, 1TK-238-3 gave tolerant to moderately tolerant reaction and 11-97k-461-4, 1T-97k-1021-15 showed moderately tolerant to susceptible reaction.
 
 
 
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