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by
M. Shokrzadeh |
Total Records (
4 ) for
M. Shokrzadeh |
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M. Shokrzadeh
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A.G. Ebadi
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In this study, we have evaluated the effects of cisplatin on the cellular total GSH level in different tumor and normal cell lines. Five different cell lines of human hepatic carcinoma (HePG2), human lung adenocarcinoma (A549), human ovarian carcinoma (SKOV3), dog kidney (LLCPK1), Chinese hamster ovary (CHO) and Human gingival fibroblast (GHF1) cell lines were exposed to their respected IC50 concentrations of cisplatin for two hours. cisplatin cytotoxicity was measured using clonogenic assay and the total cellular GSH level was analyzed using a photometrical assay. The results showed that cisplatin had different degrees of cytotoxicities on different cell lines as shown by IC50 values; o.87 for HepG2, 3.27 for A549, 0.99 for SKOV3, 5.50 for LLCPK1, 5.50 for CHO and 1.60 for GHF1 cell lines and GSH level alterations after exposure to cisplatin were also different for different cell lines that 85.33 for HepG2, 637.00 for A549, 2691.00 for SKOV3, 1388.30 for LLCPK1, 412.60 for CHO and 783.24 for GHF1 cell lines. This study showed that the cellular GSH level increased in LLCPK1, A549, SKOV3 and GHF1 cell lines, but decreased in CHO and HepG2 cell lines versus to the matched controls. The highest significant variation of GSH in cancer cell line was belonging to SKOV3 and in normal cell lines for LLCPK1, after treated with cisplatin. It is concluded that the total GSH variation after exposure to cisplatin is different for different cell lines. We were not able to correlate between the level of resistance to cisplatin (based on the IC50 levels) and GSH level or variations in this study. It might indicate the GSH neither the unique, nor the most important mechanism of resistance to cisplatin in these cell lines, in spite of many publications in its favor. |
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M.R. Kalantari
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M. Shokrzadeh
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A.G. Ebadi
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C. Mohammadizadeh
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M.I. Choudhary
and
Atta-ur-Rahman
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Heavy metals and metalloids are an increasing environmental problem worldwide. Some industrial activities and agricultural practices increase their level in the substrate and the possible introduction of these elements in the food chain is an increasing human health concern. The protection and restoration of soils and water contaminated with heavy metals generate a great need to develop efficient adsorbents for these pollutants. Agricultural fertilizers which contain small amounts of cadmium and lead is widely applied and used in Iran. However, both these heavy metals remain below toxic levels. In contrast, contamination of lowland rice fields by sewage sludge from textile plants and some mining has increased the heavy metal content of the soil and reduced rice yields in these areas (Mazandaran province). Currently remediation of polluted soil is being carried out, using plants such as Vetiveria zizanioides and Eichornia crassipes, plus applications of zeolite in some areas of Meandering province of Iran. This mini review firstly indicate general objectives about remediation and then deal with to some activies about agricultural soil remediation that contaminated with some heavy metals (specially, Pb and Cd in Mazandaran province of Iran. We conclude that above mentioned species, may be an effective species for phytoextraction and should be tested for this purpose in field conditions. |
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A.G. Ebadi
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M. Shokrzadeh
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The effect of chronic exposure to menazon (Organophosphorus compound) in the production of oxidative stress was assessed in rats. Administration of menazon (50, 100, 500, 1000 ppm) for 2 weeks duration increased catalase (CAT), superoxide dismutase (SOD) activities in red blood cells (RBC). However, acetylcholinesterase (AChE) activity were decreased in these samples. The increase in RBC lipid peroxidation correlated well with the inhibition in RBC AChE activity. Increased activities of CAT and SOD showed significant correlations in RBC samples when different doses of menazon were used. The results of the present study suggest the usefulness of RBC AChE measurement as a good index in the evaluation of menazon-induced oxidative stress affecting blood. |
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M. Shokrzadeh
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A.G. Ebadi
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In this study samples of
four species of the most consumed fishes (Sefid, koli, kilca and kafal
fish) were analyzed for concentrations of Chlorobenzilate (organochlorine
pesticides). Fish were captured using electric fishing on four sites
(Chalous and Babolsar city and Khazar Abad and Miankaleh regions) in
Mazandaran provinces of Iran. Quantitative determination of the Chlorobenzilate
was performed by gas chromatography electron-capture detection (GC–ECD).
Samples contained detectable concentrations of Chlorobenzilate but at
concentrations below the maximum residue limit (MRL). No different found
between kinds of fishes in each sites about Chlorobenzilate concentrations
but there are two groups of sites that were significantly different
from one another in terms of Chlorobenzilate concentrations. According
to insecticides analyzed in four kinds of fishes the Kafal in the hunting
region of Khazar abad had greatest amount of 0.038 PPM. Kilca from Babolsar
had the highest amount of Chlorobenzilate, 0.035 PPM (P< 0.05). In the
case of Sefid and Koli fishes in regions, significant difference was
not seen. It is necessary to mention that no research has so far been
done to be a criterion for comparison in this area sites. However, the
concentrations of Chlorobenzilate residues in the muscle were found
to be lower than the FAO/WHO recommended permissible and should not
be public concern among peoples in Mazandaran province. |
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