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Articles by M. Rabbani
Total Records ( 2 ) for M. Rabbani
  Hamid M.M. Sadeghi , M. Rabbani , A. Jafarian D. , T. Ghafghazi and H. Najafzzadeh V.
  The present study was conducted to create mutations in this motif for further investigation of its function. By using nested PCR two mutations were produced, which would translate to DRY and DKH. The PCR products as well as the PGEM3Z vector were digested using NcoI and EcoRI restriction enzymes and were ligated and transformed to E. coli HB101 cells. The obtained colonies were analyzed for the presence of the inserts using suitable restriction enzymes. The obtained plasmids have the advantage of having restriction sites, which would not interfere with further cloning and expression of this receptor using mammalian expression vectors.
  M. Rabbani , H. M. Sadeghi , A. Jafarian-Dehkordi , V. Hajhashemi and A. Rostami
  Mu Opioid receptor plays an important role in mediating most of diverse effects of opioids like analgesia, euphoria and dependence. Studies on Glycosylation Consensus of various receptors showed that it has important roles in the affinity, desensitization, G-protein coupling and intracellular signaling. The aim of the present study was to create a mutation in this consensus of Rat Mu Opioid Receptor (RMOR) for further investigation of its function. Using nested PCR one mutation was produced, N53Q. The PCR products as well as the pcDNA3 vector were digested using HindIII and BamHI restriction enzymes and were ligated and transformed to Ecoli HB101 cells. The obtained colonies were analyzed for the presence of the inserts using suitable restriction enzymes. The obtained plasmids are ready for further investigations in binding assay and function assays like cyclic AMP measurements.
 
 
 
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