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Articles by M. Mizanur Rahman
Total Records ( 2 ) for M. Mizanur Rahman
  M.A. Mamun , M.B.H. Sikdar , Dipak Kumar Paul , M. Mizanur Rahman and Md. Rezuanul Islam
  In vitro micropropagation of sugarcane variety viz., Isd-28 and Isd-29 have been studied. Nutrient media containing growth regulators enhanced callus induction, shoot differentiation and root formation in vitro. For covenant experiment, surface sterilization of the explants from the field grown plants was essential and sterilization with 0.1% HgCl2 for 8 min was satisfactory. In this treatment, 85-90% of the leaf sheath explants was Contamination free. Among the explants cultured for proliferation fortified with 3.0 mg L-1 2, 4-D explants of all the 2 varieties of sugarcane showed the best performance for the callus induction after 16-20 days of culture. The concentration of BA tested 1.5 mg L-1 of the cytokinin produced comparatively higher percentage of shoot proliferation. Different concentration of BA mg L-1 in combination with different auxin viz., IAA, IBA and NAA were tested for shoot proliferation. Isd-28 and Isd-29 showed best shooting when media were supplemented with BA 1.5 mg L-1+0.5 mg L-1 NAA. In vitro proliferated shoots were rooted on MS and modified MS media. Best results of rooting ware observed on modified MS medium supplemented with auxins (NAA+IBA) 0.5 mg L-1 and 0.5 mg L-1 showed fair results. Eighty to ninety percent regenerated plantlets were viable at normal temperature with 85% humidity while transferred sterilized soil.
  M.Z. Rahman , M.G. Sharoar , Mohammad Nurul Matin , M.H. Rahman , M. Mizanur Rahman and M. Rezuanul Islam
  Banana cultivar ‘Mehersagar’ is a dessert banana clones cultivated all over the country. For the purpose of the production of disease free plantlets suitable for large-scale cultivation in the field, a micropropagation protocol based on multiple shoot regeneration has been established. Highly proliferative cluster of multiple shoots are obtained by culturing shoot tips from ex vitro grown healthy suckers on MS medium with different concentrations and combinations of cytokinins (viz., BAP and KIN), auxins (viz., IBA, NAA and IAA) and coconut water (cw). The efficacy of these supplements on the rate of shoot multiplication was tested. Best results were obtained on MS+0.5 mg L-1 BAP+0.5 mg L-1 KIN+13% cw, where highest average number of shoots per explant was 8.2±0.236. When individual shootlets were separated and implanted in half strength MS medium with various concentrations of auxins, the better root formation were noted at 1.0 mg L-1 IBA with 6.10±0.359 roots per shoot. Then the rooted plantlets were transferred to polybags and about 90% of the acclimatized plantlets were survived when transferred to open field.
 
 
 
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