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Articles by M. Mehedi
Total Records ( 3 ) for M. Mehedi
  P. Aryal , K. M. Hossain , M. M. Ahasan , K. M. D. Islam , M. M. Billah , M. E. Islam , M. Mehedi and S. Mitra
  In the present experiment, serological haemaglutination inhibition (HI) test has been done using serum samples obtained from three breeds of chickens viz. COB-100, BV-300 and Hisex 9. For comparative evaluation of maternally derived antibody (MDA) against Newcastle disease (ND), 10 samples of COB-100 & BV-300 and 5 samples of Hisex 9 were used. All the chickens were the progeny from the parentstock that had the history of vaccination. For sero monitoring of NDV disease specific antibody in mature chickens, 10 samples, each of Hisex 9 chickens of age 34 and 48 weeks, were used for the study. The average MDA against ND were found different in the three different breeds, ranging from 98.50 in COB-100, 74.64 in BV-300 and 69.65 in Hisex 9. The level of antibody were found significantly different in two different age groups of the same breed Hisex 9 and were 98.50 in 34 weeks old chicken and 26.30 in 48 weeks old chicken respectively. The level of MDA against ND was not found significantly different in the parent stock having the history of vaccination. On the other hand, the level of antibody in mature chicken was found significantly different. The studies suggest a vaccination schedule within 10 days for a day old chicken and also close monitoring the antibody level before and after vaccination in mature chicken.
  M. Mehedi , K.M. Hossain , M.J.F.A. Taimur , B.K. Sil and M.R. Islam
  The research work was undertaken to prepare haemagglutination antigen of NDV on vero cell line as an alternative to the traditional chicken embryonated egg. Newcastle disease virus can grow within different animal cell line. Vero cell is an established cell line whose source is African green monkey`s kidney. The anchorage-dependent vero cells were first subcultured in Eagle`s minimum essential medium to form semi-confluent monolayer. This monolayer was then infected by collected passage 3 (P3) adapted NDV and maintained up to passage 7 (P7). The antigen was collected from this adapted NDV. Then the NDV antigen was assayed and tested for its purity by tissue culture infective dose (TCID50) assay and haemagglutination test respectively. The titre of NDV was 104.1 TCID50. HA result showed NDV antigen agglutinate chicken red blood cells up to 1600 dilution, which is moderately higher titre than HA titre found for NDV, propagated in chicken embryos.
  P. Shrestha , M.M. Ahasan , K.M.D. Islam , M.M. Billah , M.E. Islam , M. Mehedi , S. Mitra and M.R. Islam
  In the present experiment, Enzyme linked immunosorbent assay (ELISA) was applied on a total of 49 samples collected from 4 breeds of chicken (BV-300, Broiler Kasile, LBM and Hisex) at different age (day 1, day 5, day 10 and day 15) to determine the level of maternally derived antibody (MDA) against infectious bursal disease (IBD). All the chickens were the progeny from the parentstock that had the history of vaccination. A total number of 10 broilers were used to determine the level of IBDV specific antibody in vaccinated and in non-vaccinated chickens following infection with field virus suspension. As these chickens attained the age of 14 days, 6 chickens were vaccinated with Gumboro D78 live vaccine while remaining 4 chickens were kept without vaccination. All the chickens were infected with field virus suspension on day 19 and blood samples collected on day 29 were subjected to ELISA. Slight variation in the antibody titer was observed among 4 breeds of chickens. An average antibody titer of 5320.79, 5877.15, 3676.24 and 5581.55 was found in day old BV-300, Broiler Kasile, LBM and Hisex respectively. Day old BV-300 contained high level of MDA (average of 5320.79) and the level gradually declined and persisted up to 15-20 days. Five days old, 10 days old and 15 days old BV-300 contained an average antibody titer of 3848.57, 2615.53 and 580.88, respectively. On day 29, there was a significant level of antibody (1489.50), much above minimum protection level, in vaccinated chicken whereas nil antibody level was observed in non-vaccinated chickens. Therefore, the chicks should be vaccinated at around day 14, at which time the antibody level reaches nearly to minimum protection level. Antibody level must be carefully monitored at proper interval of time in order to make the vaccination program more effective, to keep the chickens disease free, to increase the production and to prevent the economic loss.
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