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Articles by M. M. Hussain
Total Records ( 5 ) for M. M. Hussain
  J Iqbal and M. M. Hussain
 

Our knowledge of the uptake and transport of dietary fat and fat-soluble vitamins has advanced considerably. Researchers have identified several new mechanisms by which lipids are taken up by enterocytes and packaged as chylomicrons for export into the lymphatic system or clarified the actions of mechanisms previously known to participate in these processes. Fatty acids are taken up by enterocytes involving protein-mediated as well as protein-independent processes. Net cholesterol uptake depends on the competing activities of NPC1L1, ABCG5, and ABCG8 present in the apical membrane. We have considerably more detailed information about the uptake of products of lipid hydrolysis, the active transport systems by which they reach the endoplasmic reticulum, the mechanisms by which they are resynthesized into neutral lipids and utilized within the endoplasmic reticulum to form lipoproteins, and the mechanisms by which lipoproteins are secreted from the basolateral side of the enterocyte. apoB and MTP are known to be central to the efficient assembly and secretion of lipoproteins. In recent studies, investigators found that cholesterol, phospholipids, and vitamin E can also be secreted from enterocytes as components of high-density apoB-free/apoAI-containing lipoproteins. Several of these advances will probably be investigated further for their potential as targets for the development of drugs that can suppress cholesterol absorption, thereby reducing the risk of hypercholesterolemia and cardiovascular disease.

  M. Ashraf and M. M. Hussain
  Amino acid and carbohydrate contents during early 30 minutes of wheat seed imbibition have not been reported -earlier. In this study, wheat seeds (cv. Ingelab) preimbibed in water for 2, 6, 16 and 24 hours and dried back, were soaked in water for 30 minutes and amino acid and carbohydrate contents in leachates were measured. Untreated seeds showed 4.5 times increase in amino acid contents (2-9.1 nmoles/seed) in 30 min of imbibition. However, in preimbibed seeds, as the time to pretreatment increased (from 2 to 24 h), the amount of amino acids leached decreased and in 24 h preimbibed seeds, it was 2.8 nmoles/seed after 30 min. Changes in carbohydrate contents in leachates in untreated seeds rose from 13 to 52 μg/seed in 30 min, an overall increase of 4 times was exhibited. All the pretreated seeds exhibited lower levels of carbohydrates effluxed in 30 min. However, the significant difference was observed in the initial 10 min of start of imbibition in which preimbibed seeds effluxed carbohydrate contents at faster rates (e.g. 10 times increase in 6 h and 24 h preimbibed seeds) than the control ones (0.7 times increase). Changes in these electrolytes had no effect on seed germination of these preimbibed seeds since all seeds exhibited 100 percent germination in two days. These results demonstrate that amino acid and carbohydrate contents leakage during initial 30 min of imbibition have no effect on viability of seeds but may have implications in studying seed-soil relations.
 
 
 
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