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Articles by M. Kurohmaru
Total Records ( 2 ) for M. Kurohmaru
  M. A. Awal , M. N. H. Siddiqi , M. Kurohmaru , B. B. Andriana , T. Mizukami , Y. Kanai and Y. Hayashi
  Biochemical effects of 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD) on the sertoli cell culture from prepubertal male Wistar rats were examined by light and transmission electron microscopy. Sertoli cell culture was obtained by sequential collagenase and trypsin-EDTA treatment. TCDD was added to the cell culture at the concentration of 10, 100, 500, and 1000pg ml-1, respectively and control group was treated with saline. Distinct histopathological changes in the sertoli cell culture were noted after exposure to TCDD. At 2 days after treatment, the increase of degenerated sertoli cells were 11.8, 18.7, 28.4, and 30.8% at the concentration of 10, 100, 500, and 1000pg ml-1, respectively. Similarly, at 4 days, those increased as 16.5, 28.9, 35.7 and 48.9%, and at 6 days as 25.7, 38.4, 43.7, and 69.9%, respectively. They were significantly different from those in the control group (p<0.05). The maximum degenerative change of Sertoli cells was observed at the concentration of 1000pg ml-1 and at 6 days after treatment. The research finding suggested that TCDD showed a direct effect on sertoli cells in culture. A further study is needed to elucidate the mechanism of the action of TCDD on sertoli cells.
  M.A.A. Prodan , M.R. Islam , S.K. Das , M.A. Awal , M.N.H. Siddiqi and M. Kurohmaru
  Histological studies on the arterial wall of main arteries from the ascending aorta to the pectoral and abdomino-inguinal mammary glands of indigenous dogs in different reproductive stages were observed using a light microscope. Based on the histological characteristics and organization of connective tissue fibers and smooth muscle cells in the tunica media, the arterial segments were clearly identified into elastic, transitional and muscular types. Every artery belonging to the three types had different morphological structures at different levels. The artery distant from the heart gradually decreased its elastic lamellae in the tunica media as well as its elasticity and at the periphery of the arterial tree the elastic lamellae were replaced by the smooth muscle cells. The type of the arteries does not depend on the size or diameter of the arteries but depend on the relative distance from the heart. It is assumed that the histological arrangements of tissues in the arterial walls are certainly closely related to the functional demand of the mammary glands.
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