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Articles by M. Khayami
Total Records ( 5 ) for M. Khayami
  R. Heidari , M. Khayami and T. Farboodnia
  In this research we exposed four day old seedlings of Zea mays were exposed to different concentrations of 0.25, 0.5, 1, 2 mM Pb(NO3)2 with pH 6 in the controlled condition. After 72 h the root of seedlings were harvested and the changes of fatty acids and total lipids percent of the samples were dermined. The percent of total lipids has been increased specially in 2mM Pb treatment. It was suggested that this was not because of inducing the process of lipids synthesis, but it was due to inhibition of growth, loss of metabolism activities and metabolite synthesis. The changes of fatty acids indicated elevated amount of unsaturated linoleic and linolenic fatty acids; which are considered as a tolerance mechanism to protect membrane systems against oxidative and heavy metals stress. But increased level of K+-leakage according to Pb concentration referred to significant decline of membrane stability. So it was concluded that Pb is a major chemical pollutant that causes different damages to root cells structure and components and Zea mays is a Pb-tolerant plant with a high potential of adaptation mechanisms like producing higher unsaturated fatty acids and the ability of forming some advantious roots from the first node of shoot that helps it to tolerate stress conditions.
  R. Heidari , M. Khayami and T. Farboodnia
  In this research we examined the effect of 0.25, 0.5 and 1 mM Pb(No3)2 with and without 0.5 mM EDTA in pH 4 and 6 on the growth and Pb accumulation in the root and shoot of four day old seedlings of Zea mays at the controlled condition in the period of 72 h. At the end of treatment we determined the length and dry weight of the root and Pb content in the root and shoot of the seedlings. In the second test we considered specially the effect of 0.25, 0.5, 1, 1.5 and 2 mM EDTA with 0.5 mM Pb(No3)2 on the seedlings of Zea mays at the same condition. After 72 h we determined the amount of Pb by Atomic Absorption and EDTA by HPLC and water content of shoot. We concluded (from the results of two tests) that: Pb absorption has grown up parallel to its concentration in growth solution and has inhibited root growth and biomass significantly. Pb taking up in pH 4 was higher than pH 6 and EDTA enhanced Pb accumulation in shoot. But water content of shoot decreased at the concentrations more than 0.5 mM EDTA in growth solution. Maximum level of EDTA accumulated in shoot of plants was at the concentration of 1.5 mM EDTA in the culture, but the highest level of Pb and the least water loss of shoot was at the equimolar Pb and EDTA (0.5 mM Pb with 0.5 mM ETDA). It may concluded that EDTA is taken up by plants, accumulated in shoots in the form of Pb-EDTA or protonated form and enhanced Pb accumulation in shoots of seedlings. So it can be used for phytoextraction of Pb and other metals not only by accumulators but also by tolerant plants such as Zea mays. But if EDTA is used for supplying plant micronutrients, its concentration should be minimized and its damage to plants should be clearly considered.
  M.A. Bakhshayeshi , M. Khayami , R. Heidari and R. Jamei
  The anthocyanin pigments was extracted from the four Malus varieties such as M. domestica cv. red starking,M. domestica cv. red delicious, M. domestica cv. jonathan and M. domestica cv. abbasi mashhad using the soaking in Ethanol (1% acidified).The extracted anthocyanin pigments then were exposed to number of environmental factors which could destabilize the anthocyanin molecules. These environmental factors were included three different pH (1, .5 and 4), four various temperatures (5, 0, 0 and 40°C) and presence or absence of light.The results showed that increasing in pH, temperature or exposure to light is able to destruction the anthocyanin pigments. Another factor affecting the tolerance of anthocyanin towards the environmental condition is the role of different varieties. Among the various Malus varieties anthocyanin pigment in M. domestica cv. red starking, showed the greatest resistance to destruction by environmental conditions, other varieties followed: M. domestica cv. red delicious M. domestica cv. jonathan and M. domestica cv. abbasi mashhad consecutively.
  E. Nikkhah , M. Khayami , R. Heidari and I. Bernousi
  Anthocyanins are natural pigments widely distributed in nature. Anthocyanin color molecules are a subclass of flavonoids. They are responsible for the reds, purples and blues in many flowers, fruits and vegetables. Fruits and berries are the most sample sources of anthocyanins in nature. Berries and fruits are an important part of the Finnish diet. In many researches the positive effect of fruit and berry intake on human health has been reported. Anthocyanins are considered to contribute to the healthiness of fruits and berries for their antioxidant, anti-carcinogenic, anti-inflammatory and anti-angiogenic properties for example. Anthocyanins can also improve the nutritional value of processed foods by preventing oxidation of lipids and proteins in the food products. However, the stability of anthocyanins becomes most significant also in this case, as in the case of color quality. In this study the anthocyanin pigment was extracted from the three different berries (Morus nigra L., Morus alba var. nigra and Fragaria L.). Using the soaking and wetting in ethanol (1% acidified). The extracted anthocyanin pigments then were exposed to three different concentration of SO2 (25, 50, 100 ppm). Three groups of anthocyanin solutions keeping in darkness and refrigerator for 63 days and per 3 week, the quantity of anthocyanin absorbance recorded in 520 nm.
  N. Fekri , M. Khayami , R. Heidari and R. Jamee
  Mucilage, a complex carbohydrate with a great capacity to absorb water, should be considered a potential source of industrial hydrocolloid.To examine moisture, dry weight, yield, ash and protein content, mucilaginous material were extracted from seeds by mixing the seeds with distilled water (1:20 w/v), stirring the seed-water mixtures for 3 h at 75C, separating the mucilage extract from the seeds by filtration through a muslin and were precipitated with 3 times its volumes of 96% ethanol. The precipitates were separated by centrifugation (6500 rpm, 15 min). The data were analyzed by one-way ANOVA and Tukey test and compared in one significant levels of p< 0.05. The linseed mucilage had 4.57% moisture, 95.43% dry weight, 8.6% yield, 5.8% ash and 12.3% protein. The basil mucilage had 4.86% moisture, 95.14% dry weight, 2.07% yield, 0.84% ash and 10.9% protein. The dragon head mucilage had 4.93% moisture, 95.06% dry weight, 1.88% yield, 0.28% ash and 45.7% protein. The quince mucilage had 4.38% moisture, 95.62% dry weight, 10.9% yield, 8.24% ash and 20.9% protein. Results showed significant differences in yield mucilage content between flaxseed and quince with together and with basil and dragon head. Significant differences in ash content were observed between flaxseed and quince with basil and dragon head. The dragon head had significant difference in protein content with flaxseed, basil and quince.
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