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Articles by M. Javed Zaki
Total Records ( 9 ) for M. Javed Zaki
  Amer-Zareen , M. Javed Zaki and Nazir Javed
  Aqueous ginger extract was tested against root knot nematode Meloidogyne javanica. In vitro studies, higher concentrations of extract (100% concentration) suppressed root knot egg hatching and caused juveniles mortality. Pasteuria endospore attachment was greater in 25% concentration extracts compared to those at 100 and 50% concentrations. Plant growth improved and disease severity was reduced when combined applications of the plant extract and P. penetrans were applied. In treatments receiving 25% extract and P. penetrans, 72% females were parasitized by the bacterial antagonist and 9 spores of bacteria per juvenile were found attached with nematode cuticle.
  Shabbar Hussain , Amer-Zareen , M. Javed Zaki and M. Abid
  All test cultivars of chickpea respondedto root knot nematode M. javanica. Plant tops in nematode inoculated pots were significantly hampered (at p<0.01) compared to nematode free control. Maximum suppression in plant height by nematode was observed in cv. Nes 95 0174 followed by cv. 91 A039, whereas maximum suppression in shoot weight was observed in cv. Nes 95 0012 & Nes 95 0174 in response to nematode infection. Lower degree of gall formation per root system was observed in cv. Nes 95 004. Whereas cv. 90 122, 93 127 & Nes 950174 limited nematodedensitiesin soil and root compared to rest of cultivars. According to the host response index (0- >10), all test cultivars were moderately resistant. Reproduction factor ( r) was higher in cv. Nes 96 003 (r =1.88) compared to rest ofcultivars. In biocontrol experiment CF of A. nigerw as effectively increased plant height, fresh shoot weight and root length followed by A. flavus and A. temarii compared to other treatments and control. Maximum reduction in nematodesoil density was achieved by A.flavus (>17%)followed by A. temarii (134%) compared to other treated pots. Gall formation per root system was significantly hampered (at p<0.001) where A. niger was applied followed by A. nidulans> A. flavus> A. terreus> A. fumigatus compared to control.
  Amer-Zareen , Nargis Jamil Khan and M. Javed Zaki
  In a greenhouse study P. lilacinus, T. harzianum and T. flavus were used as seed treatment and soil drench. Seed treatment by P. lilacinus reduced gall formation, egg mass production, nematode soil and root densities as compared to control and other treatments. P. lilacinus improved plant growth followed by T. flavus and T. harzianum comparing with control. In another experiment, where soil was treated with conidial suspension, maximum plant height and shoot weight was achieved by P. lilacinus > T. harzianum compared to control, whereas T. flavus was found less effective in the enhancement of plant tops. Maximum suppression in gall formation (at p <0.01) and egg mass production (at p<0.001) was obtained in okra plants treated with P. lilacinus whereas T. flavus and T. harzianum were almost equally effective. Conidial suspension of microbial antagonists used as soil amendment also reduced nematode root invasion (at p<0.001) as well as soil densities
  Amer-Zareen , M. Javed Zaki and Nargis Jamil Khan
  Seven species of Aspergillus were screened for their biocontrol potential against Meloidogyne javanica root-knot nematodes on tomato under greenhouse conditions. Culture filtrates (CF) of Aspergillus niger, A. terreus and A. tamarii improved plant height, root length and fresh shoot weight when used as soil drench or bare root dipped seedlings. Besides, maximum reduction in gall formation and egg mass production was obtained by CF of A. niger, A. fumigatus and A. terreus in both soil drench and bare root dip seedlings compared to control. A. niger was found most successful in minimizing root and soil densities of root-knot nematodes in soil drench treatments compared to other test CF and control. Bare root dipped seedlings by A. nidulans CF reduced the root-knot development in tomato roots and in rhizosphere.
  Imran A. Siddiqui , Amer-Zareen , M. Javed Zaki and S. Shahid Shaukat
  The efficacy of Trichoderma viride, T. harzianum, T. hamatum, T. koningii and T. pseudokoningii was tested for the control of Meloidogyne javanica, root knot nematode in okra and mungbean. Culture filtrates of Trichoderma spp., significantly reduced egg hatching and showed nematicidal activity by killing second stage juveniles of M. javanica. Soil application with conidial suspension of T. harzianum significantly reduced nematode population densities and root knot development in okra and mungbean. Apart from suppressing root knot nematode, T. harzianum also elevated plant height and fresh shoot weight of both okra and munhbean.
  Imran Ali Siddiqui , Amer-Zareen , S. Shahid Shaukat and M. Javed Zaki
  Efficacy of different species of rhizobia including, Rhizobium trifolii, R. phaseoli, R. meliloti, Bradyrhizobium japonicum and Bradyrhizobium sp., were tested in the control of Meloidogyne javanica, the root-knot nematode on Vigna mungo under laboratory, greenhouse and field conditions. Cell-free culture filtrates of the rhizobium species significantly reduced egg hatching and caused mortality of M. javanica larvae In vitro. In pot experiments and under field conditions, Bradyrhizobium sp., gave better biocontrol along with enhanced plant growth and nodulation.
  Imran Ali Siddiqui , Syed Ehteshamul-Haque , M. Javed Zaki and Abdul Ghaffar
  Pseudomonas aeruginosa, a plant growth-promoting rhizobacterium and Memnoniella echinata, a fungal antagonist used separately or in combination in soil amended with neem cake and/or chemical fertilizers significantly suppressed root infecting fungi viz., Macrophomina phaseolina, Fusarium solani and Rhizoctonia solani and the root-knot nematode Meloidogyne javanica in mungbean. Soil amendment increased biocontrol and growth promoting potential of P. aeruginosa and M. echinata.
  Amer Zareen , M. Javed Zaki , S. Shahid Shaukat and S. R. Gowen
  Pasteuria spore attachment to root knot juveniles reduced significantly (p<0.001), as dilution factor of bacterial suspension was increased. Isolate UK 1 provided maximum spore attachment to Meloidogyne juveniles in all three dilutions of bacterial spore suspension (S, S/2 and S/4) compared to other test isolates and control. Infected juveniles by spores of all bacterial isolates (UK 1, PK 1 and PK 2) significantly (p<0.05) suppressed invasion to tomato roots. There was significant difference (p<0.001) in root invasion by nematodes exposed to the different concentrations of test bacterial isolates. Temperatures i.e. 15 and 35°C exhibited reduction in bacterial spore attachment to the root knot juveniles. Whereas 25 and 30°C favored bacterial infection of nematodes.
  Amer Zareen , Nazir Javed and M. Javed Zaki
  In a mirco-plot field experiment four isolates of Pasteuria (one from UK and three local isolates viz., PK 1, PK2 and PK3) and a mixed inoculum of all four isolates (blend) were tested against Meloidogyne javanica on tomato to understand the pathogenic variability of different bacterial isolates. Yield of tomato and plant growth as plant height, fresh shoot and root length was improved significantly by blend inoculum of bacterial antagonists compared to control. Nematode development in roots and soil was suppressed by blend applications followed by individual Pasteuria applications i.e. UK > PK 1> PK2 and >PK3 isolates compared to untreated control plots.
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