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Articles by M. Habibur Rahman
Total Records ( 3 ) for M. Habibur Rahman
  M. Habibur Rahman , Md. Salim Uddin , Nural Islam , Farjana Nikkon and M. Fida Hasan
  Amylase was purified from the juice of healthy and diseased sugarcane, Ishurdi (Isd)-20 by successive chromatographies on DEAE-Sephadex A-50 and Sephadex G-150 to a homogeneous state as confirmed by polyacrylamide disc gel electrophoresis (PAGE). The molecular weights of the enzyme from healthy and diseased sugarcane juices were estimated as 55.8 and 56.3 kDa, respectively by gel filtration. The purified amylases were of -type and not a glycoprotein in nature. The purified amylases showed the following characteristics, respectively; Km values- 0.27% and 0.23%; optimum pH 6.4 and 6.3; and optimum temperature 39 and 40°C for starch as substrate. Ca2+, Mg2+, and Mn2+ increased amylase activities remarkably. The activities of amylases were increased slightly by Zn2+. On the other hand Cd2+ and Fe3+ increased moderately. Na+ had almost no effect on the amylase activity. Ag+ and K+ produced a little inhibitory effect but Cu2+ inhibited amylase activities moderately. EDTA and acetic acid almost completely ceased amylase activities. Both amylases were found to contain 70% activity in presence of 8M urea.
  Farjana Nikkon , Zahangir Alam Saud , M. Habibur Rahman and Md. Ekramul Haque
  The research work was conducted to investigate the in vitro antimicrobial activity of the compound Aglycon of Deoxy-Niazimicine (N-benzyl,S-ethyl thioformate) isolated from Moringa oleifera Lam. in comparison with crude chloroform extract. Antibacterial and antifungal activities of the compound-1 and crude extract were observed against fourteen pathogenic bacteria and six pathogenic fungi. Compound-1 isolated from the chloroform (CHCl3) fraction of ethanol extract showed comparatively more in vitro antibacterial activity against Shigella boydii, Shigella dysenteriae and Staphylococcus aureus than the crude chloroform (CHCl3) extract and produced zone of inhibition in between 09 and 13 mm. The MIC values of the compound-1 and crude extract were also determined against B. megaterium, St. aureus, Sh. dysenteriae and Sh. boydii. The values were found to be between 32 to 128 μg ml-1. The compound-1 also showed moderate antifungal activity against Candida albicans and Aspergillus flavus than the crude chloroform extract although the crude extract showed moderate activity against Trichoderma species. Thus compound-1 has antibacterial and antifungal activity and suggested its therapeutic use as an antimicrobial agent.
  S.M.M. Mahbubur Rahman , Palash Kumar Sen , M. Fida Hasan , M.A. Samad Miah and M. Habibur Rahman
  Sugarcane invertase was purified from mature sugarcane stem tissue to an electrophoretically homogenous state, by successive ion-exchange chromatography on DEAE-Cellulose and CM-Cellulose column chromatography. The molecular weight of purified invertase enzyme was determined 218 kDa on SDS-Polyacrylamide slab gel electrophoresis. When the enzyme was characterized, it was found that the sugarcane invertase is glycoprotein in nature and it contained 7.29% sugar. The height enzyme activity was found at pH 7.2 and 60°C temperature. The Km value of the enzyme was 8.0 mM. NaCl, MnCl2 increased invertase activity remarkably and KCl increased slightly while MgCl2, CaCl2 increased moderately. HgCl2 has completely and FeCl2 showed strong inhibitory effects on enzyme activity while EDTA and acetic acid have remarkable inhibitory effects on invertase activity. CuCl2, ZnCl2 and CdCl2 decreased invertase activity moderately. AgNO3, AlCl3, Glucose and Tris showed less inhibitory effects on invertase activity.
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