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Articles by M. Ekramul Haque
Total Records ( 7 ) for M. Ekramul Haque
  M. Saiful Islam , M. Badrul Alam , Ronok Zahan , G.C. Sarker , N.S. Chowdhury , M. Maruf Khan , Mahedi Hasan , M. Saifur Rahman , M.A. Mosaddik , Mele Jesmin and M. Ekramul Haque
  The present study was designed to investigate the antioxidant and anti neoplastic activities of Ocimum sanctum Linn. (OS) leaves. Antioxidant potential of the OS extract was evaluated in vitro by DPPH (1, 1diphenyl-2-picrylhydrazyl) and NO (Nitric Oxide) scavenging assay and reducing power assay method. OS extract showed prominent scavenging activity in all the methods with IC50 value of 16.39±0.31 and 16.20±0.33 μg mL-1 for DPPH and NO assay method, respectively. In reducing power assay, OS extract also showed significant (p<0.001) activity. In addition, total phenolic and flavonoid content and total antioxidant capacity were also determined. The anti neoplastic effect of the leaves of OS against Ehrlich Ascites Carcinoma (EAC) in mice at the doses of 50 mg kg-1 body weight intraperitoneally. Significant (p<0.001) increases of survival times 33±1.81 days for crude extract of the (50 mg kg-1) treated tumor bearing mice were confirmed with respect to the control group (20±0.12 days). Hematological studies reveal that the Heamoglobin (Hb) content was decreased in EAC treated mice whereas restoration to near normal levels was observed in extract treated animals. There was a significant (p<0.001) decrease in RBC count and increase in WBC counts in extract/fraction treated animals when compared to EAC treated animals. From the result it was showed that the extract has significant antioxidant as well as anti neoplastic activity.
  Ronok Zahan , M. Badrul Alam , M. Saiful Islam , Gopal C. Sarker , Nargis S. Chowdhury , Salman B. Hosain , M.A. Mosaddik , Mele Jesmin and M. Ekramul Haque
  The research study was conducted to determine the antitumor effect of the flower of Alangium salvifolium (crude extract and diethylether fractions) against Ehrlich Ascites Carcinoma (EAC) in mice at the doses of 10 mg kg-1 body weight intraperitoneally. Extract/fractions was administered for nine consecutive days. Twenty-four hours of last dose and 18 h of fasting, the mice were sacrificed and antitumor effect was assessed by evaluating tumor volume, viable and nonviable tumor cell count, tumor weight and hematological parameters of EAC bearing host. Significant (p<0.001) increases of survival times 30±0.96 and 25±0.40 days for crude extract and diethylether fraction of the A. salvifolium (10 mg kg-1) treated tumor bearing mice, respectively were confirmed with respect to the control group (20±0.13 days). The extract/fraction also decreased the body weight of the EAC tumor bearing mice. Hematological studies reveal that the heamoglobin (Hb) content was decreased in EAC treated mice whereas restoration to near normal levels was observed in extract treated animals. There was a significant (p<0.001) decrease in RBC count and increase in WBC counts in extract/fraction treated animals when compared to EAC treated animals. From the result it was showed that the extract has significant anticancer activity and that is comparable to that of Bleomycin.
  M. Habibur Rahman , M. Badrul Alam , N.S. Chowdhury , M.K. Jha , M. Hasan , M.M. Khan , M.S. Rahman and M. Ekramul Haque
  In the present study crude methanolic extract of Stephania japonica leaf was investigated for possible antioxidant, analgesic and toxic activity. The extract showed antioxidant activity in DPPH radical scavenging activity, nitric oxide scavenging activity and reducing power assays. In both DPPH radical and NO scavenging assay, the extract exhibited moderate antioxidant activity and the IC50 values in DPPH radical scavenging and NO scavenging assays were found to be 105.55±1.06 and 129.12±0.15 μg mL-1, respectively while the IC50 values of ascorbic acid were 12.30±0.11 and 18.64±0.22 μg mL-1, respectively. Reducing power activity of the extract increased in a dose dependent manner. Analgesic activity of the crude extract was evaluated using acetic acid-induced writhing model of pain in mice. The crude extract at 200 and 400 mg kg-1 b.wt. doses displayed significant (p<0.001) reduction in acetic acid induced writhing in mice with a maximum effect of 75.89% reduction at 400 mg kg-1 b.wt. which is comparable to the standard, diclofenac sodium (86.52%). The extract was also investigated for toxic potentiality using Brine Shrimp lethality bioassay. In this bioassay the extract showed significant toxicity to Brine Shrimp nauplii with the LC50 value of 25.19±0.98 μg mL-1. The study clearly indicates that the extract possesses good analgesic and cytotoxic activity along with moderate antioxidant potential.
  M. Badrul Alam , M. Sarowar Hossain , N. Sultana Chowdhury , M. Asadujjaman , Ronok Zahan , M. Monirul Islam , M. Ehsanul Haque Mazumder , M. Ekramul Haque and Anwarul Islam
  The present study was designed to investigate the antioxidant, anti-inflammatory and antipyretic potential of the methanolic extract along with its organic soluble fractions of the fruits of Trichosanthes dioica Roxb. Antioxidant potential of the extract/fractions was evaluated by DPPH (1,1diphenyl-2-picrylhydrazyl) and NO (nitric oxide) scavenging assay method. Ethyl acetate fractions (EtOAc) showed highest scavenging activity in all the methods with IC50 value of 12.32±0.16 and 5.38±0.07 μg mL-1 for DPPH and NO assay method, respectively. In reducing power assay, EtOAc fraction also showed significant (p<0.001) activity. Further, the extract/fractions were studied for their anti-inflammatory (carrageenan induced paw edema in rats) and antipyretic (Brewer’s yeast induced pyrexia) activities at a dose level of 100, 200 and 400 mg kg-1 body weight for MeOH extract. Methanolic extract showed a dose dependent and significant (p<0.005, p<0.05) anti-inflammatory and antipyretic effect. Dichloromethane fraction (CH2Cl2) and Ethyl acetate fractions exhibited similar activity using a dose of 200 mg kg-1 b.wt. in these models. The pharmacological activities of the (CH2Cl2) fraction were lesser than the MeOH extract and other fractions. In addition, total phenolic and flavonoid content and total antioxidant capacity were also determined. Altogether, these results suggest that the MeOH extract and its organic soluble fractions EtOAc could be used as a potential antioxidant, anti-inflammatory and antipyretic agent.
  M. Badrul Alam , M. Sarowar Hossain , Nargis Sultana Chowdhury , M. Ehsanul Haque Mazumder , M. Ekramul Haque and Anwarul Islam
  In course of investigation on natural antioxidants, the present study was aimed to report the antioxidant activities, both in vitro and in vivo, of the crude methanolic extracts of the whole plant of Oxalis corniculata Linn along with its various organic fractions. The different assay methods, including total antioxidant activity, scavenging free radical, authentic peroxynitrite, nitric oxide and reducing power assessment were used to evaluate the antioxidant potential of the crude extract and its organic fractions. The ethylacetate (EtOAc) fraction, showed strong activity in all the model systems tested and in peroxynitrite model this fraction (IC50 value of 2.29±0.18 μg mL-1) exerted three-fold stronger activity than standard penicillamine (IC50 value of 6.20±0.32 μg mL-1). The reducing power of the extract was found to be concentration dependent. The administration of the extract/fractions at a dose of 250 and 500 mg kg-1 body weight to the male Wistar rats increased the percentage inhibition of reduced glutathione, superoxide dismutase and catalase significantly. Whereas, lipid peroxidation level in hepatotoxic rats markedly decreased at a dose of 500 mg kg-1 body weight after 7 days. The total phenol and flavonoid content were also measured in the crude extract along with its organic fractions. The Brine shrimp lethality bioassay was used to determine the toxicity of the extracts and Vincristin sulphate was used as positive control. The dichloromethane (CH2Cl2) fraction showed highest activity (LC50 value of 29.02±1.16 μg mL-1) and other showed activity in the order of: EtOAc fraction >n-BuOH fraction> MeOH extract > aqueous fraction. Taken together, these results suggest that O. corniculata extract has strong antioxidant properties and further validate the traditional use of this plant.
  Farjana Nikkon , Kazi Abdus Salam , Tanzima Yeasmin , Ashik Mosaddik , Proma Khondkar and M. Ekramul Haque
  Two triterpenes, β - amyrin and 12- oleanene 3β, 21β - diol, were isolated as a mixture from the chloroform soluble fraction of an ethanol extract of Duranta repens Linn (Verbenaceae) stem. The structures of the two compounds were confirmed by analysis of their IR, 1H - NMR, 13C - NMR and LC - MS spectral data. The mixture of β - amyrin and 12 - oleanene 3β, 21β - diol (compound 1) was highly effective against the larvae of the mosquito, Culex quinquefasciatus Say (Diptera: Culicidae), as a mosquitocide.
  Ishrat Jahan , M. Abdullah A. Mamun , M. Atowar Hossen , J.A.M. Samir Sakir , M. Shamimuzzaman , M. Jashim Uddin and M. Ekramul Haque
  Nymphaea nouchali leaves are used in folk medicine for the treatment of various disorders. In order to evaluate the actions of this plant, studies were performed on antioxidant, analgesic and anti-inflammatory activities. The Methanolic extract of Nymphaea Nouchali Flowers (MNNF) was evaluated for anti-inflammatory activity using carrageenan induced hind paw edema model whereas hot plate, writhing and formalin tests was carried out for analgesic activity. Total phenolic and flavonoids content, scavenging of 1, 1-Diphenyl-2- Picrylhydrazyl (DPPH) radical, peroxynitrate (ONOO-) as well as inhibition of total ROS generation were used to evaluate antioxidant potential of MNNF. The extract MNNF at the dose of 100 and 200 mg kg-1, produced a significant (p<0.05) increase in pain threshold in hot plate method whereas significantly (p<0.05) reduced the writhing caused by acetic acid and the number of licks induced by formalin in a dose dependent manner. The same ranges of doses of MNNF caused significant (p<0.05) inhibition of carrageenan induced paw edema after 4 h in a dose dependent manner. In DPPH, ONOO- and total ROS scavenging method, MNNF showed good antioxidant potentiality with the IC50 value of 10.33±1.02, 20.16±0.61 and 31.72±0.48 μg mL-1, respectively. The findings of the study suggested that Nymphaea nouchali has strong analgesic, anti-inflammatory and antioxidant effects, conforming the traditional use of this plant for inflammatory pain alleviation to its antioxidant potentiality.
 
 
 
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