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Articles by M. Daliri
Total Records ( 2 ) for M. Daliri
  B. Mahmoudi , M.Sh. Babayev , M. Daliri , M. Nejadgashti and S. Esmaeelkhanian
  In this study the genetic variation among 6 Iranian native goats population was investigated using 10 microsatellite markers (LSCV38, LSCV41, LSCV36, TGLA122 MAF64, oarFCB304, oarJMP23, oarAE133, BM121 and BM4621) every 10 loci were amplified successfully but the data resulting from the LSCV38 locus was ignored because of a large number of Null allele during the analysis steps. The rest nine loci in all populations were 100% polymorphic. Lori (LOR), Raeeni (RAN), Najdi (NAJ) and Korki Jonoob Khorasan (KAJ) populations did not present no deviation of Hardy-Weinberg Equilibrium (HWE). Morkhoz (MOR) and Tali (TAL) population presented no deviation from HWE in locus-population components (p<0.005) but considering all populations did not observe deviation from HWE (p<0.005). MOR and NAJ population had the highest and the lowest genetic variation within population 0.847 and 0.725, respectively. Unbiased average Heterozygosity (He) was expected by Nei. TAL and NAJ population had the lowest genetic distance (0.2728) and the highest genetic distances considering above criteria were for LOR and TAL population (0.7448). Phylogeny tree based on UPGMA method with DS criterion has been put Iranian goat population in 2 great clusters. Assignment test, considering the analysis nine loci, assigning individual 2 population verify high confidence.
  M. Dashtizad , H. Wahid , O. Abas Mazni , Y. Rosnina , M. Daliri and H. Hajarian
  Development of efficient culture system to support embryonic development would be valuable when percentage of produced embryos reaching to the blastocyst stage is important. However, the rate of bovine embryo production in vitro is still lower than expected. Present study was performed to investigate the effect of ghrelin on nuclear maturation and subsequent bovine embryo development in vitro. Cumulus-oocyte-complexes were collected from slaughterhouse ovaries and randomly allocated in each treatment groups. Five different concentrations of ghrelin (0, 5, 50, 500 and 1000 ng mL-1) were added to the in vitro maturation medium (Hepes-buffered medium 199+fetal calf serum+gonadotrophins+insulin+antibiotics). The proportion of oocytes developed to metaphase II stage was significantly increased at 5 and 50 ng mL-1 ghrelin (86.32±3.38 and 89.77±2.92%, respectively). The result also indicated that adding high concentration of ghrelin adversely affect (p<0.05) the nuclear maturation rates of bovine oocytes. However, the subsequent embryo development was not significantly affected by addition of ghrelin to the IVM medium. This study showed that inclusion of 5-50 ng mL-1 ghrelin in maturation medium may have beneficial effects on nuclear maturation of bovine oocytes in vitro.
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