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Articles by M. Ayub Ali
Total Records ( 6 ) for M. Ayub Ali
  G. Patra , T.K. Rajkhowa , Lalhruaitluangi Sailo , W. Ramdas Singh , Gunjan Das , Kh. Victoria Chanu and M. Ayub Ali
  A biochemical study was made to elucidate the biochemical composition of the inner wall of oocyst of Eimeria necatrix and then compared with cellular component of the oocyst. In addition to total protein, glucose and cholesterol, several electrolyte components have been estimated in the present study. The investigation also revealed high activity of Alanine and Asparate aminotransferases in the inner wall of the oocyst of Eimeria necatrix. It seems that the biochemical composition and the enzymes in the inner wall of the oocyst of Eimeria necatrix are of significant value for better metabolic processes in Eimeria spp. as well as for better understanding of possible drug-parasite interaction.
  Gautam Patra , M. Ayub Ali , Kh. Victoria Chanu , L. Jonathan , L.K. Joy , M. Prava , R. Ravindran , G. Das and L. Inaotombi Devi
  The present investigation was undertaken to evaluate histo-pathological, haemotological and biochemical changes in broiler chicken naturally infected with Eimeria tenella. A Polymerase Chain Reaction (PCR) based assay was also done for the detection, identification and differentiation of pathogenic species of Eimeria in poultry. Post mortem examination revealed petechial haemorrhages, oedema, necrosis and sloughing of caecal epithelium. Histopathological evidence showed leakage of blood, oedema and necrosis. Haemotological studies revealed that coccidial infection caused significant decrease in the value of haemoglobin and Packed Cell Volume (PCV). The value of Mean Corpuscular Volume (MCV) and Mean Corpuscular Haemoglobin Concentration (MCHC) revealed that caecal coccidiosis resulted in macrocytic hypochromic anaemia. Biochemical serum analysis of coccidial infected chicken showed a significant increase in level of glucose, Alanine Aminotransferase (ALT) and Aspartate Aminotransferase (AST) while there was a significant decrease in the level of alkaline phosphatase (SAP) and cholesterol. In PCR using specific primers for E. tenella only the ITS 1 regions of ribosomal DNA (rDNA) could be amplified.
  Irungbam Karuna Devi , Y. Geeta Devi , Kh. Victoria Chanu , M. Ayub Ali , Jully Gogoi Tewari , M. Prava , David Malsawmkima and Gautam Patra
  The present work describes isolation, cell wall preparation, morphological as well as molecular detection of oocyst of Eimeria tenella. Based on transmission electron microscopy, the oocyst of this species showed the outer and inner wall without veil forming bodies; cross section of sporozoites with different organelles and macrogamete structure at ultrastructural level. Polymerase Chain Reaction (PCR) based on Internal Transcribed Spacer 1 (ITSI) regions of ribosomal DNA (rDNA) demonstrated 270 bp specific for E. tenella. Results of this study provide the basis for further ultrastructural studies of the organism. The present study also suggested that morphological characteristics along with molecular identification are prerequisite for validation and identification of Eimeria species.
  Gautam Patra , W.M. Lyngdoh , M. Ayub Ali , M. Prava , Kh. Victoria Chanu , T.C. Tolenkhomba , Gunjan Das , H. Prasad , L. Inaotombi Devi and Irungbam Karuna Devi
  A study was carried out in broiler chicken for comparative anthelmintic efficacy of neem and pineapple leave powder with piperazin hydrate for treatment of experimental Ascaridia galli infection. The affected birds had lower levels of Hb, PCV, Ca and P. The profile changed after treatment with neem and pineapple leave powder which was comparable to that observed after treatment with piperazine hydrate. Neem and pineapple leave powder treatment caused cent percent evacuation of the worms on 28th and 56th day post treatment respectively.
  M. Ayub Ali , L. Inaotombi Devi , W.M. Lyngdoh , Gunjan Das , H. Prasad , Kh. Victoria Chanu , M. Prava , T.C. Tolenkhomba , Y. Damodar Singh and M.C. Lallinchhunga
  A study was carried out to evaluate the change in serum biochemical profile of broiler chickens experimentally infected with Ascaridia galli on treatment with powdered leaves of neem and pineapple and with piperazin hydrate. The control and birds treated with piperazine, neem and pineapple show changes in the level of glucose, cholesterol, total protein, albumin, magnesium, potassium and SGPT. The piperazine treated groups can resist better the decreased in the glucose level compare to the groups treated with neem and pineapple. The serum cholesterol level of the control and treated birds shows increased in level while the total protein and albumin contents decreased in control and treated groups. However the decreased in the neem treated group was higher for total protein while the pipeapple treated group shows higher decrease in the level of albumin. The serum level of the potassium and SGPT level also changes in control as well as treated groups.
  Gautam Patra , M. Ayub Ali , Kh. Victoria Chanu , Jonathan Lalsiamthara , J.L. Kataria , Snigdha Hazarika , David Malsawmkima , R. Ravindran and L. Inaotombi Devi
  An outbreak of coccidiosis in laboratory rats has been described in this study. Clinically, out of 50 rats, 30 were died after showing symptoms of anorexia, emaciation and diarrhea. Post mortem examination showed the large and small intestine distended with necrotic and sloughed off mucosal epithelial cells with intestinal content. The examination of the gut contents revealed numerous Eimeria oocysts. After sporulation, the oocysts were identified as those of Eimeria nieschulzi. The infection due to Eimeria nieschulzi is confirmed by Polymerase Chain Reaction (PCR) after amplifying partial (~1.6 kb) nuclear 18S rDNA from DNA of E. nieschulzi. The histopathological examination showed severe damage of the lamina propria of the intestinal mucosa with numerous coccidian developmental stages in the epithelium of small intestine. Biochemical analysis of serum from infested rats presented a significant increase in serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST), glucose, total proteins, globulin and inorganic phosphorous while a decrease in alkaline phosphatase and blood urea nitrogen with insignificant changes in potassium, sodium, magnesium and chloride.
 
 
 
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