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Articles by M. Asok Kumar
Total Records ( 2 ) for M. Asok Kumar
  P. Thomas , T.R. Arun , K. Karthik , P.V. Berin , M. Asok Kumar , Neetu Singh , J. Usharani , M. Palanivelu , S.K. Gupta , K. Dhama and K.N. Viswas
  Necrotic enteritis, caused by Clostridium perfringens, is an important bacterial disease of poultry. A suspected case of necrotic enteritis was presented for necropsy from an Indian Kadaknath Fowl flock showing diarrhea and progressive debility. Gross examination revealed necrotic to ulcerative lesions in intestine. The organism was isolated from the intestinal contents, tissue and liver under anerobic conditions. The cultural characteristics and Gram staining were suggestive of C. perfringens. The sequencing of 16s rRNA gene confirmed the isolate as C. perfringens and which was well differentiated from other clostridia associated with avian intestinal tract. This study demonstrates that 16s rRNA gene sequencing can provide rapid and confirmatory identification of C. perfringens. Further, Multiplex Polymerase Chain Reaction (mPCR) was performed for toxinotyping and isolate was found to be positive for α toxin (cpa) and β2 toxin (cpb2), a feature of C. perfringens type A isolates. As some recent studies have highlighted the involvement of NetB toxin in pathogenesis, therefore, PCR was carried out to find the presence of this toxin, the isolate was found to be negative for netB gene. This study emphasizes the molecular characterization and toxinotyping as a rapid tool for detection of C. perfringens from suspected necrotic enteritis cases. Very few reports regarding molecular characterization are available from India, hence it adds to the available data on this important poultry pathogen. Further investigations are required to understand the exact role of NetB toxin in pathogenesis as various studies including the current one reports NetB negative strains involved in necrotic enteritis.
  Kuldeep Dhama , Shambhu Dayal Singh , Rajamani Barathidasan , P.A. Desingu , Sandip Chakraborty , Ruchi Tiwari and M. Asok Kumar
  Growth in poultry sector is being challenged due to increased incidence and re-emergence of diseases caused due to evolution of several viral pathogens and use of live vaccines. Piles of economic losses are encountered due to these diseases. Avian Infectious Bronchitis (IB), caused by Corona virus, is OIE-listed disease and characterized by respiratory, renal and urogenital involvements, causing high mortality. Economic losses are encountered due to loss of productive performance of both egg and meat-type chickens. Variant viruses evolve due to spontaneous mutations and recombinations, causing disease in vaccinated flocks of all ages. Serotyping and genotyping are the common methods of classification of IBV strains. The virus has 4 clusters, grouped into 7 serotypes and the most important strains are Massachusetts, Connecticut, Arkansas, Gray, Holte and Florida along with numerous others, distributed round the globe. Several conventional and molecular diagnostic methods have been described for the diagnosis of IB in chickens. 'All-in/all-out' operations of rearing along with good biosafety measures forms the basis of prevention, whereas vaccination forms the backbone of IB control programme. Both live and inactivated (oil emulsified) conventional vaccines are available. The new generation vaccines (recombinant and vector-based) developed against locally prevailing IBV strains may be more helpful and avoid the reversion of virulence in live vaccine viruses. The present review deals with all these perspectives of this important emerging poultry pathogen.
 
 
 
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