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Ten multidrug resistant Salmonella typhi were isolated from the blood and stool samples of clinically suspected patients suffering from typhoid fever. To see whether the drug resistance phenomenon was plasmid mediated, one of these resistant isolates was used for the plasmid analysis and a single 20 kb plasmid was found. This 20 kb plasmid was transferred to a sensitive E. coli LE 392 and after the plasmid transfer experiment E. coli LE 392 acquired resistance against those previously used antibiotics. Plasmid analysis of the transformed E. coli LE 392 further exhibited that it harbored a single 20 kb plasmid corresponding to that of the wild S. typhi which further confirmed that the 20 kb plasmid was carrying the gene(s) responsible for the multidrug resistance in Salmonella typhi.
The shoots of Rauvolfia serpentina from the field growing plants were used as explants and cultured on MS medium supplemented with 2.5 mg/L BA + 0.1 mg/L NAA to afford multiple shoots. It was evident that MS + 0.5 mg/L BA + 0.2 mg/L NAA was the most suitable combination for induction of callus and development of shoot buds formation but it failed to support elongation of shoot. So, the cultures with tiny shoots was transferred to 2.0 mg/L BA with 0.05mg/L NAA, where they showed excellent elongation. Regenerated shoots rooted well 0.2 mg/L IBA+ 0.2mg/L NAA combination. After having acclimatization the regenerated plantlets were transferred to the open field where 95% of the plant-lets survived and showed excellent growth.
