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Articles by M. Aminafshar
Total Records ( 2 ) for M. Aminafshar
  E. Sharifi Seidani , C. Amirinia , A. Lavaf , C. Farasati and M. Aminafshar
  In order to investigate genetic variation of 3 ecotypes of Sanjabi sheep (Zardi, Kajal, Kolul), an experiment was planned, using 10 microsatellite markers (INRA023, SPS113, HSC, D5S2, McM527, MAF65, INRA005, INRA063, oarFCB304 and oarFCB11). Genomic DNA was extracted from 150 blood samples, using modified Salting out method. All loci were amplified by PCR except INRA023. Loci in all of the ecotypes were highly polymorphic. Significant departures from HWE were detected for all loci in all population, except for oarFCB11 in Kolul ecotype (p<0.05). INRA063 was the most polymorphic marker according to its effective number of alleles (equal to 6.58 in Zardi), expected heterozygosity (equal to 0.86 in Zardi), Shannon information index (equal to 2.01 in Zardi) and Polymorphism information content (equal to 0.83 in Zardi). However, MAF65 showed the lowest effective number of alleles (equal to 2.91 in Kajal), expected heterozygosity (equal to 0.66 in Kajal), Shannon information index (equal to 1.18 in Kajal) and Polymorphism information content (equal to 0.59 in Kajal). According to the observations, the Zardi ecotype was more polymorphic and showed highest genetic variation than the others as well as Kolul was lowest. The phylogenetic clusters presented that Zardi and Kolul ecotypes have minimum Genetic Distance to each other. Results showed that, high level of genetic diversity was observed in all ecotypes of Sanjabi breed and this breed was not at risk for conservation concept.
  M. Aminafshar , C. Amirinia and R. Vaez Torshizi
  Genetic Structure of Guilan buffalo population, in the South and South western area of Caspian Sea in Iran, was characterized, using 14 microsatellite markers (CSSM019, CSSM029, CSSM033, CSSM038, CSSM041, CSSM043, CSSM047, CSRM060, CSSM061, CSSM062, CSSM070, BMC1013, BRN and ETH003). The average of observed and effective number of alleles across all loci was found to be 4.14 and 3.17, respectively. CSSM062 was the most polymorphic markers according to its effective number of alleles (4.69), expected heterozygosity (0.79) and Polymorphism information content (0.75). However, CSSM029 showed the lowest effective number of alleles (1.20), expected heterozygosity (0.50) and Polymorphism information content (0.37). Results showed that these markers were suitable in population genetics researches. The mean of observed and expected heterozygosity, Polymorphism information content and Shannon information index were equal to 0.90, 0.67, 0.61 and 1.22, respectively across all loci in the population. It was concluded that a high degree of genetic diversity exist in the Guilan buffalo populations.
 
 
 
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