|
|
| |
| Articles
by
M. Abdollahi |
Total Records (
3 ) for
M. Abdollahi |
|
 |
| |
|
| |
M. Ghazi-Khansari
,
M. Karami
,
M. Rezayat
,
M. Abdollahi
,
B. Minaie
and
O. Sabzevari
|
| |
The purpose of this study was to evaluate protective effects of vitamin E, selenium and propranolol on TCDD induced changes of biochemical parameters using the rat liver perfusion system. Various concentrations of TCDD (0.3, 3, 20, 30 μg L-1) were added to the perfusion fluid and biochemical changes in perfusion fluid of isolated rat liver were examined within 2 h. The results showed that TCDD significantly increases the aminotransferase activities in a dose-dependent manner. It was determined that TCDD at 20 μg L-1 caused a maximum increase in biochemical parameters within 2 h (p<0.0001). The result also showed that propranolol (20 mg L-1), sodium selenite (345 mg L-1) and vitamin E (α-tocopherol, 700 mg L-1) significantly decreases TCDD hepatotoxicity. Aminotransferase enzyme activity as well as glutathione and protein content significantly changed in treatment groups as compared to the TCDD group (p<0.001). Reduction in hepatotoxicity may be attributed to prevention of lipid peroxidation, although other mechanisms may also be involved. |
|
| |
|
| |
M. Mohajer
,
P. Sarkhail
,
N. Hajarolasvadi
,
M.J. Zamani
,
R. Khorasani
,
A. Shafiee
,
G. Amin
and
M. Abdollahi
|
| |
The purpose of this investigation was to study the anti-inflammatory and
analgesic properties of total extract and four fractions (ether, ethyl acetate,
n-butanol and water) from Phlomis lanceolata (Lamiaceae) in mice.
The plant material was extracted with methanol. In order to estimate the
polarity of the active compounds, the total extract was dissolved in water and
the water soluble portion was successively partitioned between ether, ethyl
acetate and n-buthanol. The total extract and four fractions were
analyzed by Thin Layer Chromatography (TLC) by use of specific reagents. Dose of
100 mg kg 1 of each extracts were used in carrageenan-induced paw
edema, formalin and writhing nociception tests in mice. All compounds reduced
paw edema in comparison to the control group at 1, 3, 5 and 7 h post carrageenan
injection. The total, ether and aqueous extracts were similar to indomethacin
while the ethyl acetate extract was weaker than indomethacin in reduction of paw
edema. All extracts induced antinociception in both phases of formalin test. The
total and ether extracts were as potent as indomethacin in both phases of
formalin test. The ethyl acetate extract was weaker than indomethacin in the
second phase of formalin-test while the n-butanol and aqueous extracts
showed more antinociception than indomethacin in the second phase of formalin
test. All extracts as well as indomethacin induced antinociception in writhing
test in comparison to control. The total and aqueous extracts induced the same
antinociception as indomethacin while ether, ethyl acetate and n-butanol
showed weaker antinociception than indomethacin. Positive results for iridoids
and phenolic compounds were indicated by phytochemical analysis of total
extract. Phenolic compounds were found in four fractions whereas only n-butanol
and aqueous fractions showed positive results for iridoid glycosides. The higher
antinociceptive effects of n-butanol and aqueous extracts in the
inflammatory phase of formalin test among different extracts tested, might back
to the presence of iridoid glycosides, phenolic glycosides or other glycosides.
These data suggest that different extracts of P. lanceolata produce
different antinociceptive activities that could be due to the effect of one or a
combination of the bioactive components in each extract. |
|
|
| |
|
| |
M. Skokrzadeh
,
F.H. Shirazi
,
M. Abdollahi
,
A.G. Ebadi
and
H. Asgarirad
|
| |
One of the well-known cellular defenses after exposure to cytotoxic agents is the glutathione (GSH) related mechanisms. Resistant to cisplatin (DP) chemotherapy has been strongly correlated to GSH-mediated mechanisms in many articles. In this study, we have evaluated the effects of cisplatin on the cellular total GSH level in different tumor and normal cell lines. Five different cell lines of human hepatic carcinoma (HePG2), human lung adenocarcinoma (A549), human ovarian carcinoma (SKOV3), dog kidney (LLCPK1), Chinese Hamster Ovary (CHO) and Human gingival fibroblast (GHF1) cell lines were exposed to their respected IC50 concentrations of cisplatin for two hours. Cisplatin cytotoxicity was measured using clonogenic assay and the total cellular GSH level was analyzed using a photometrical assay. The results showed that cisplatin had different degrees of cytotoxicities on different cell lines as shown by IC50 values; 0.87± 0.07 for HepG2, 3.27±0.35 for A549, 0.99±0.08 for SKOV3, 5.50±0.35 for LLCPK1, 5.50±0.21 for CHO and 1.60±0.21 for GHF1 cell lines. GSH level after exposure to cisplatin (GSH-DP) were also different for different cell lines compare to their controls (GSH-C); 85.33±8 for HepG2, 637.00±81 for A549, 2691.00±416 for SKOV3, 1388.30±261 for LLCPK1, 412.60±32 for CHO and 783.24±30 for GHF1 cell. It is shown that compare to the matched controls, the cellular GSH level increased in LLCPK1, A549, SKOV3 and GHF1 cell lines, but decreased in CHO and HepG2 cell lines. The highest significant variation of GSH in cancer cell line was belonging to SKOV3 and in normal cell lines to LLCPK1, after treated with cisplatin. It is concluded that the total GSH variation after exposure to cisplatin is different for different cell lines. We were not able to correlate between the level of resistance to cisplatin (based on the IC50 levels) and GSH level or variations in this study. It might indicate that in spite of many publications so far, the GSH is neither the unique, nor the most important mechanism of resistance to cisplatin in these cell lines. Internal and Eternal GSH level in Studied cell lines will be changed in several ways when contaminated with different concentration of vitamins (for examples, Vit C, Vit E and Vit C+E) and observed that variation was more prominent in cancer cell line. |
|
|
|
|
| |
|
