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Articles by M Schuster
Total Records ( 3 ) for M Schuster
  T deVos , R Tetzner , F Model , G Weiss , M Schuster , J Distler , K. V Steiger , R Grutzmann , C Pilarsky , J. K Habermann , P. R Fleshner , B. M Oubre , R Day , A. Z Sledziewski and C. Lofton Day
 

Background: The presence of aberrantly methylated SEPT9 DNA in plasma is highly correlated with the occurrence of colorectal cancer. We report the development of a new SEPT9 biomarker assay and its validation in case–control studies. The development of such a minimally invasive blood-based test may help to reduce the current gap in screening coverage.

Methods: A new SEPT9 DNA methylation assay was developed for plasma. The assay comprised plasma DNA extraction, bisulfite conversion of DNA, purification of bisulfite-converted DNA, quantification of converted DNA by real-time PCR, and measurement of SEPT9 methylation by real-time PCR. Performance of the SEPT9 assay was established in a study of 97 cases with verified colorectal cancer and 172 healthy controls as verified by colonoscopy. Performance based on predetermined algorithms was validated in an independent blinded study with 90 cases and 155 controls.

Results: The SEPT9 assay workflow yielded 1.9 µg/L (CI 1.3–3.0) circulating plasma DNA following bisulfite conversion, a recovery of 45%–50% of genomic DNA, similar to yields in previous studies. The SEPT9 assay successfully identified 72% of cancers at a specificity of 93% in the training study and 68% of cancers at a specificity of 89% in the testing study.

Conclusions: Circulating methylated SEPT9 DNA, as measured in the new mSEPT9 assay, is a valuable biomarker for minimally invasive detection of colorectal cancer. The new assay is amenable to automation and standardized use in the clinical laboratory.

  M Schuster , M Pints and M. Fiege
  Background

Prehospital emergency care is provided in many European countries by specialised emergency physicians. However, little is known about the impact of experience and educational level of emergency physicians on providing prehospital care.

Methods

During a 6-month period all deployments of an emergency physician-staffed ambulance in a metropolitan area were studied according to possible predictors of prehospital mission times.

Results

In the univariate comparison the junior emergency physicians had 6.3-minute longer prehospital mission times than senior emergency physicians. This difference was evident in several National Advisory Committee for Aeronautics (NACA) score subgroups. However, in multivariate analysis, patient conditions, like NACA score, Glascow coma scale or prehospital diagnosis had by far the more significant impact on mission times.

Conclusion

The effect of education on treatment process and outcome in prehospital emergency care should merit further research, especially to ensure that junior emergency physicians are properly trained before they work in prehospital emergency medicine.

  K. D Pruitt , J Harrow , R. A Harte , C Wallin , M Diekhans , D. R Maglott , S Searle , C. M Farrell , J. E Loveland , B. J Ruef , E Hart , M. M Suner , M. J Landrum , B Aken , S Ayling , R Baertsch , J Fernandez Banet , J. L Cherry , V Curwen , M DiCuccio , M Kellis , J Lee , M. F Lin , M Schuster , A Shkeda , C Amid , G Brown , O Dukhanina , A Frankish , J Hart , B. L Maidak , J Mudge , M. R Murphy , T Murphy , J Rajan , B Rajput , L. D Riddick , C Snow , C Steward , D Webb , J. A Weber , L Wilming , W Wu , E Birney , D Haussler , T Hubbard , J Ostell , R Durbin and D. Lipman
 

Effective use of the human and mouse genomes requires reliable identification of genes and their products. Although multiple public resources provide annotation, different methods are used that can result in similar but not identical representation of genes, transcripts, and proteins. The collaborative consensus coding sequence (CCDS) project tracks identical protein annotations on the reference mouse and human genomes with a stable identifier (CCDS ID), and ensures that they are consistently represented on the NCBI, Ensembl, and UCSC Genome Browsers. Importantly, the project coordinates on manually reviewing inconsistent protein annotations between sites, as well as annotations for which new evidence suggests a revision is needed, to progressively converge on a complete protein-coding set for the human and mouse reference genomes, while maintaining a high standard of reliability and biological accuracy. To date, the project has identified 20,159 human and 17,707 mouse consensus coding regions from 17,052 human and 16,893 mouse genes. Three evaluation methods indicate that the entries in the CCDS set are highly likely to represent real proteins, more so than annotations from contributing groups not included in CCDS. The CCDS database thus centralizes the function of identifying well-supported, identically-annotated, protein-coding regions.

 
 
 
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