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| Articles
by
Lorena Rodriguez |
Total Records (
2 ) for
Lorena Rodriguez |
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J. Cantizani
,
J. Ortiz
,
A. S. Ravipati
,
Lorena Rodriguez
,
B. Cautain
,
L. Zhang
,
N. Reddy
,
C. E. Nath
,
F. Vicente
,
N. de Pedro
and
S. R. Koyyalamudi
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Background: The aim of this study is screening Chinese medicinal plants
for inhibitors of Glycogen synthase kinase-3 (GSK-3). GSK-3 is a proline/serine
protein kinase ubiquitously expressed and involved in many cellular signalling
pathways. GSK-3 has emerged as one of the most attractive therapeutic targets
for the development of selective inhibitors as promising new drugs for numerous
pathologies, including neurodegenerative diseases and type II diabetes. Thus,
the use of GSK-3 inhibitors is one of the most promising therapeutic strategies
for the future treatment of these potentially life threatening diseases. Materials
and Methods: In the aim of discovery of potential inhibitors, 42 traditional
Chinese medicinal plants were screened against GSK-3β which were selected
based on their folklore use. The selected plant materials were extracted with
ethanol and water. In vitro assay was carried out to evaluate the inhibition
of human GSK-3β. The Methylthiazolyldiphenyl-tetrazolium bromide (MTT)
assay was conducted with immortalized Hepatocyte cell line (Fa2N4) to evaluate
the cytotoxicity of the plant material. Results: Many new ethanol and
aqueous extracts showed significant inhibitory activity against GSK-3β
with moderate or no cytotoxicity. Water extracts of Prunella vulgaris, Rabdosia
rubescens and Sarcandra glabre have exhibited highest inhibition
against GSK-3β. This in turn was supported by the fact that a good correlation
exists between GSK-3β inhibitory activity and antioxidant content of the
extracts. Conclusion: Considering the potent activity of P. vulgaris,
R. rubescens and S. glabre, further isolation and characterization
of individual bioactive compounds is recommended for the discovery of potent
natural inhibitors of GSK-3β. |
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Nuria De Pedro
,
Bastien Cautain
,
Juan Cantizani
,
Lorena Rodriguez
,
Francisca Vicente
,
RatnaTualsi
and
Sundar Rao Koyyalamudi
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Background: The mitochondrion is an essential organelles for producing most of the energy in the cell and also involved in a number of cellular activities in the cell. It plays an important role in the aging process. Mitochondrial dysfunctions are supposed to be responsible for many neurodegenerative diseases as Alzheimer’s Disease (AD), Parkinson’s Disease (PD) and Amyotrophic Lateral Sclerosis (ALS). A growing body of evidence suggests that defects in mitochondrial metabolism and particularly of electron transport chain may play a role in neurodegenerative diseases. Secondary effects as proapoptotic factors and Reactive Oxygen Species (ROS) can be an early stage of several mitochondrial disorders. Objective: To evaluate the mitochondrial membrane potential, ROS generation, GSK-3β and CK-1δ activities of seven natural products (isoquercetin, rutin, avicularin, hesperetin, astragalin, luteolin and diosmin) by using well established assays in vitro assays. Materials and Methods: In this study, neuroprotective effect of seven natural products on rotenone-induced toxicity in a SH-SY5Y neuroblastoma cells model for PD. Results: In this study, seven natural products were assessed the mitochondrial membrane potential, ROS generation, GSK-3β and CK-1δ activities using well established assays. The most effective compound to prevent mitochondrial membrane depolarization and decrease the ROS levels was luteolin followed by isoquercetin and astragalin. Isoquercetin, astragalin, hesperetin and rutin have good activity against GSK-3β and no activity against CK-1δ. Conclusion: This study results suggest that isoquercetin and astragalin exerts its neuroprotective effect against rotenone due to its mitochondrial membrane potential, ROS generation, GSK-3β and CK-1δ activities. |
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