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Articles by Lorena Rodriguez
Total Records ( 2 ) for Lorena Rodriguez
  J. Cantizani , J. Ortiz , A. S. Ravipati , Lorena Rodriguez , B. Cautain , L. Zhang , N. Reddy , C. E. Nath , F. Vicente , N. de Pedro and S. R. Koyyalamudi
  Background: The aim of this study is screening Chinese medicinal plants for inhibitors of Glycogen synthase kinase-3 (GSK-3). GSK-3 is a proline/serine protein kinase ubiquitously expressed and involved in many cellular signalling pathways. GSK-3 has emerged as one of the most attractive therapeutic targets for the development of selective inhibitors as promising new drugs for numerous pathologies, including neurodegenerative diseases and type II diabetes. Thus, the use of GSK-3 inhibitors is one of the most promising therapeutic strategies for the future treatment of these potentially life threatening diseases. Materials and Methods: In the aim of discovery of potential inhibitors, 42 traditional Chinese medicinal plants were screened against GSK-3β which were selected based on their folklore use. The selected plant materials were extracted with ethanol and water. In vitro assay was carried out to evaluate the inhibition of human GSK-3β. The Methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay was conducted with immortalized Hepatocyte cell line (Fa2N4) to evaluate the cytotoxicity of the plant material. Results: Many new ethanol and aqueous extracts showed significant inhibitory activity against GSK-3β with moderate or no cytotoxicity. Water extracts of Prunella vulgaris, Rabdosia rubescens and Sarcandra glabre have exhibited highest inhibition against GSK-3β. This in turn was supported by the fact that a good correlation exists between GSK-3β inhibitory activity and antioxidant content of the extracts. Conclusion: Considering the potent activity of P. vulgaris, R. rubescens and S. glabre, further isolation and characterization of individual bioactive compounds is recommended for the discovery of potent natural inhibitors of GSK-3β.
  Nuria De Pedro , Bastien Cautain , Juan Cantizani , Lorena Rodriguez , Francisca Vicente , RatnaTualsi and Sundar Rao Koyyalamudi
  Background: The mitochondrion is an essential organelles for producing most of the energy in the cell and also involved in a number of cellular activities in the cell. It plays an important role in the aging process. Mitochondrial dysfunctions are supposed to be responsible for many neurodegenerative diseases as Alzheimer’s Disease (AD), Parkinson’s Disease (PD) and Amyotrophic Lateral Sclerosis (ALS). A growing body of evidence suggests that defects in mitochondrial metabolism and particularly of electron transport chain may play a role in neurodegenerative diseases. Secondary effects as proapoptotic factors and Reactive Oxygen Species (ROS) can be an early stage of several mitochondrial disorders. Objective: To evaluate the mitochondrial membrane potential, ROS generation, GSK-3β and CK-1δ activities of seven natural products (isoquercetin, rutin, avicularin, hesperetin, astragalin, luteolin and diosmin) by using well established assays in vitro assays. Materials and Methods: In this study, neuroprotective effect of seven natural products on rotenone-induced toxicity in a SH-SY5Y neuroblastoma cells model for PD. Results: In this study, seven natural products were assessed the mitochondrial membrane potential, ROS generation, GSK-3β and CK-1δ activities using well established assays. The most effective compound to prevent mitochondrial membrane depolarization and decrease the ROS levels was luteolin followed by isoquercetin and astragalin. Isoquercetin, astragalin, hesperetin and rutin have good activity against GSK-3β and no activity against CK-1δ. Conclusion: This study results suggest that isoquercetin and astragalin exerts its neuroprotective effect against rotenone due to its mitochondrial membrane potential, ROS generation, GSK-3β and CK-1δ activities.
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