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Articles by Ling Zhu
Total Records ( 3 ) for Ling Zhu
  Zhicai Zuo , Shan Liao , Xinqiao Qi , Yue Yi , Zhiwen Xu , Ling Zhu , Wanzhu Guo and Hengmin Cui
  BCL-10 is an important functional and an apoptotic regulator gene inducing the organismic innate immunity. It has not been reported that the BCL-10 protein of porcine in normal level could motivate immune factor to produce. Bioinformatics analysis was performed to predict the characteristics of BCL-10, the results indicated that BCL-10 gene encoded a polypeptide, molecular mass of 45 Ku and comprising 233 amino acids. The prediction of secondary structure showed that BCL-10 had 9 alpha-helix, 1 beta-sheet, 12 turn and 14 random coil. The protein had twenty transmembrane regions, an CARD structure field which located in 15-100 amino acids and no signal peptide, the hydrophobic index was between -2.500 and 1.400. The phylogenetic tree showed that BCL-10 was similar to human (the homology index was >95%) and had a distant relationship to mouse. To identify the activity of BCL-10, the protein with a His-tag was expressed in E. coli Rosetta and purified. The purified protein showed an obvious effect of cell proliferation. Cloning and analysising of BCL-10 gene which laid foundation to further research and exploitation.
  Desheng Li , Lei Chen , Chengdong Wang , Hongbo Dai , Mengliang Liu , Ling Zhu , Xiaoyu Wang , Zhiwen Xu , Wanzhu Guo and Heng Min Cui
  The nasal bacterial flora of seven giant pandas was identified by 16S ribosomal RNA (rRNA) gene sequencing analysis. The most widely occurring species were Staphylococcus and Escherichia coli. Several species not known to be associated with giant pandas were present in the study including Pantoea agglomerans, Corynebacterium auriscanis, Kurthia gibsonii and Corynebacterium glutamicum.
  Yingzhi Wang , Lei Chen , Desheng Li , Ling Zhu , Wanzhu Guo and Zhiwen Xu
  A Gram-positive coccus isolated from a Giant Panda was identified by 16S ribosomal RNA (rRNA) gene sequencing analysis. Phylogenetic analysis showed that the organism was a member of the family Rothia. The 16S rRNA gene sequence of the organism showed 100% similarity with R. nasimurium strain CCUG 35957. Animal experiments were conducted to study the pathogenicity of R. nasimurium SCGP. This is the first reported isolation of R. nasimurium from the upper respiratory tracta of Giant Panda.
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