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Articles by Ling Zhao
Total Records ( 9 ) for Ling Zhao
  Xun Wang , Haifeng Liu , Ling Zhao , Han Dong , Yuhao Wang , Di Sun , Yuyin Liu , Ruqi Yang and Xuewei Li
  To study the effects of Crude Polysaccharides from Catathelasma ventricosum (CVCP) on proliferation and differentiation of 3T3-L1 preadipocyte. 3T3-L1 cells were treated with culture media contained different concentrations of CVCP (0, 150, 300 and 600 μg mL-1) for 24 h and subsequently cell proliferation was assayed by MTT Method. The extent of the differentiation was determined by fluescence spectrophotometry and Nile Red staining on days 8 after treatment with culture media supplemented with different concentration CVCP (0, 150, 300 and 600 μg mL-1). As the results, the different concentration CVCP (150, 300 and 600 μg mL-1) promoted proliferation of 3T3-L1 preadipocytes when compared with the control (p<0.05). And it stimulated cell differentiation into adipocyte at low concentration (150 μg mL-1) while it inhibited cell differentiation into adipocyte at higher concentration (300 and 600 μg mL-1). But the difference was not significant (p>0.05). Besides, lipid content in 3T3-L1 cells treated with 150 μg mL-1 was significantly higher than treated with 600 μg mL-1 (p<0.05). It can be concluded from the results that the CVCP promoted proliferation of the 3T3-L1 cells 24 h after treatment. Meanwhile, it was involved to some extent in regulation of adipogenesis and lipogenesis.
  Ling Zhao , Kaiyu Wang , Dexiang Wang , Chang Liu and Wei Zhang
  Microwave-assisted extraction was investigated for extraction of polysaccharides from Catathelasma ventricosum using Response Surface Methodology (RSM). The effects of operating conditions (extraction time, microwave power and ratio of water to raw material) on the extraction yield of polysaccharides were studied. A Box-Behnken Design was used for experimental design and analysis of the results to obtain the optimal processing parameters. Among the three extraction parameters, the microwave power was the most significant factor to affect the yield of polysaccharides from Catathelasma ventricosum. In addition, microwave power and extraction time had significant interaction effects. A mathematical model with high determination coefficient was gained and could be employed to optimize polysaccharides extraction and the optimal conditions were extraction time 5 min, microwave power 420 W, ratio of water to raw material 25:1. Under these conditions, the experimental yield of polysaccharide was 7.83±0.19% which was close to the predicted value.
  Xun Wang , Haifeng Liu , Ling Zhao , Han Dong , Zhengxin Yan , Hengmin Cui and Xuewei Li
  Constitutive Androstane Receptor (CAR) is one of members of the nuclear receptors superfamily which is involved in induction of drug metabolism and lipogenic pathway. In this study, the reseachers investigated whether the mRNA expression pattern of CAR in porcine preadipocyte is changed during the process of induced differentiation. Preadipocytes were aseptically taken from 3 days old piglet under general anesthesia and grown to near confluence. Postconfluent cells (Day 0) were further cultured in differentiation medium for 3 days. From day 3 onward, the cells were cultured in maintain-medium based on differentiation medium without roglitazone and IBMX until day 15. The total RNA was isolated on seven time points (i.e., days 0, 2, 4, 6, 8, 10 and 15). The mRNA expression changes of the CAR before and after porcine primary preadipocytes differentiation that acorss seven time points (i.e., days 0, 2, 4, 6, 8, 10 and 15) were measured using the quantitative RT-PCR method. In addition, the researchers also examined lipid accumulation and the mRNA expression of adpocyte differentiation markers (Such as PPARγ and RXRα) which confirmed the differentiation of preadipocytes to adipocytes. The results showed that the mRNA expression of CAR was up-regulated during porcine primary preadipocyte differentiation. There is the reason that the CAR may be involved in the process of preadipocyte differentiation and is a potential drug target for the treatment of obesity and diabetes.
  Ling Zhao , Kaiyu Wang , Ruqi Yang , Xun Wang , Defang Chen and Fei Shi
  To deeply explore the pharmacological activities of the Chinese truffle Tuber sinense (CT) in this study, three kinds of the crude polysaccharides from Chinese Truffle Tuber sinense (CTCP) were extracted by the method of water extracting-alcohol precipitating which include Water-soluble Polysaccharide (W-CTCP) and Alkali-soluble Polysaccharides (A-CTCPI, A-CTCPII) and then the polysaccharide content of the CTCP were determined. The antioxidant activities of the CTCPs were evaluated by reducing power, superoxide radical and hydroxyl radical free radical-scavenging assay. As the results, the polysaccharide (W-CTCP, A-CTCPI and A-CTCPII) content was 46.76, 74.05 and 57.68%, respectively. The polysaccharides all exhibited antioxidant activities in vitro and W-CTCP showed the strongest activity. It can be concluded that the polysaccharides extracted from CT should be explored as a potential natural antioxidant agent for use in functional foods or medicine.
  Ling Zhao , Kaiyu Wang , Xun Wang , Dan Huang , Juchun Lin , Defang Chen , Gang Ye and Cheng Lv
  In this study, Response Surface Methodology (RSM) was employed to optimize the extraction conditions (extraction temperature, ratio of water to raw material and extraction time) of polysaccharides from the fruiting bodies of Chinese truffle Tuber sinense. A Box-Behnken design was used for experimental design and analysis of the results to obtain the optimal processing parameters. The optimal conditions were extraction temperature 94.75°C, ratio of water to raw material 16.41:1, extraction time 2.67 h. According to analysis, extraction temperature was the most significant factor to affect the yield of polysaccharides from Chinese truffle Tuber sinense. Pharmacological experiments indicated that CTP had potent immunomodulatory effects in immunosuppression mice model. At the dose of 50, 100 and 200 mg kg-1 body weight, a significant increase (p<0.01) in phagocytosis of macrophage was observed.
  Xun Wang , Hengmin Cui , Haifeng Liu , Ling Zhao , Xi Peng , Hengyong Xu , Yan Wang , Yuhao Wang , Ling Niang and Qian Rong
  To study the effect of the crude polysaccharides from Poria cocos (PCCP) on mRNA expression of adipogenic factors during 3T3-L1 preadipocyte differentiation. 3T3-L1 preadipocytes were induced to differentiate with MDI (3-isobutyl-1-Methylxanthine, Dexamethasone and Insulin) induction media contained PCCP (50 μg mL-1) and cultured for 8 days. The extent of the differentiation was assessed by Nile Red staining and GPDH activity. In addition, researchers used real-time quantitative PCR to analyzed the expression of genes related to adipocyte differentiation. The results showed that treatment of differentiating 3T3-L1 preadipocytes with PCCP (50 μg mL-1) resulted in an increase in the expression of the adipocyte genes such as Fatty Acid System Synthase (FAS), adipocyte fatty acid binding Protein (aP2), insulin-responsive Glucose Transporter (GLUT4) and transcriptional factor genes such as CCAAT/Enhancer Binding Protein α (C/EBPα), peoxisome Proliferator-Activated Receptor gamma (PPARγ) and Sterol Regulatory Element-Binding Protein 1c (SREBP-1c) to some degree. Overall, the data suggested that there was the tendency of increasing the expression of genes related to adipocyte differentiation in PCCP treated cells.
  Xun Wang , Haifeng Liu , Ling Zhao , Han Dong , Chunyou Ning , Xuewei Li and Hengmin Cui
  To study the effects of crude polysaccharides from Poria cocos (PCCP) on proliferation and differentiation of 3T3-L1 preadipocyte. 3T3-L1 cells were treated with culture medium contained different concentrations of PCCP (0, 50, 100 and 200 μg mL-1) for 48 h and subsequently cell proliferation was assayed by MTT Method. The extent of the differentiation was determined by fluescence spectrophotometry and Oil Red O staining on days 8 after treatment with culture medium supplemented with different concentration PCCP (0, 50, 100 and 200 μg mL-1). As the results, the different concentration PCCP (50, 100 and 200 μg mL-1) inhibited to some extent proliferation of 3T3-L1 preadipocytes when compared with the control. But the difference is not significant (p>0.05). And PCCP stimulated cell differentiation into adipocyte at lower concentration (50 and 100 μg mL-1) (p<0.05) while it inhibited cell differentiation into adipocyte at high concentration (200 μg mL-1) (p>0.05). It can be concluded from the results that the PCCP was involved in regulation of adipogenesis and lipogenesis.
  Ling Zhao , Kaiyu Wang , Qingjiao Liao , Rui Zhang and Wei Zhang
  In order to maximize the yield of polysaccharides of Catathelasma ventricosum, response surface methodology was applied to optimize the extraction conditions (extraction temperature, ratio of water to raw material and extraction time) of polysaccharides from the fruiting bodies of Catathelasma ventricosum. A box-behnken design was used for experimental design and analysis of the results to obtain the optimal processing parameters. Among the three extraction parameters, the extraction temperature was the most significant factor to affect the yield of polysaccharides from Catathelasma ventricosum. A mathematical model with high determination coefficient was gained and could be employed to optimize polysaccharides extraction and the optimal conditions were extraction temperature 100°C, ratio of water to raw material 22.78:1, extraction time 3.66 h. Under these conditions, the experimental yield of polysaccharide was 6.47±0.14% which was close to the predicted value.
  Yi Tang , Zhongyu Liu , Ling Zhao , Thomas L. Clemens and Xu Cao
  β-Catenin functions both as an adherens junction adhesion protein and as an essential mediator of the canonical Wnt signaling pathway. Wnts stabilize β-catenin and promote its accumulation in the nucleus, where it regulates transcription of the target genes. Here we show that Smad7 promotes cell-cell adhesion by stabilizing β-catenin and consequently increases the β-catenin-E-cadherin complex level at the plasma membrane. A Smad7-Axin interaction disassociates GSK-3β and β-catenin from Axin, as well as inhibits the recruitment of Smurf2, an E3 ligase, to β-catenin, thus protecting β-catenin from phosphorylation and degradation. Smad7 increases the stabilized β-catenin to form a complex with E-cadherin and stabilizes the E-cadherin-β-catenin complex. Thereby, rather than being translocated to the nucleus for regulating the target gene transcription, Smad7-stabilized-β-catenin is shunted to the E-cadherin complex to modulate cell-cell adhesion.
 
 
 
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