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Articles by Lin Zhang
Total Records ( 16 ) for Lin Zhang
  De-Fang Zeng and Lin Zhang
  In the study, a novel soybean seed-coating agent was prepared which used carboxymethylchitosan as the main component, complemented with trace elements, trace fertilizers, penetrating agent, etc, including high ratio of performance-to-price and environmental friendliness. It is efficient and effective, resulting in better seed and crop protection. The influence of coating film on germination energy, germination percentage, seedling growth, antifeedant rate, safety performance, and field growth of the soybean plants was studied in a series of tests and are described in this paper. The effect of increasing yield of this coating agent was tested in experiments conducted in both laboratory and field. Result of the tests showed that the crop yield of soybean seeds coated by the novel seed-coating agent was increased by 17.95%, and at 25.75% less cost, as compared with a traditional soybean seed-coating agent. The antifeedant test of the novel seed-coating agent showed that it had an excellent antifeedant effect and a unique efficacy in pest control as determined by the analysis of feeding area of the leaves and selective antifeedant rate. The acute toxicity test on Sprague-Dawley (SD) rats confirmed that it was safe for the public and meets environmental requirements during usage and disposal so it may show obvious economic and environmental benefits.
  Lin Zhang , Jianming Liu , Hongzhou Li and Zhiyong Peng
  Ad hoc On-demand Distance Vector (AODV), Dynamic Source Routing (DSR) and Temporally Ordered Routing Algorithm (TORA) are three on-demand routing protocols for ad hoc networks. This study described the principles of the three protocols. The theoretical models of nodes distribution are then introduced. Finally, the effectiveness of routing protocols in wireless ad hoc networks is discussed based on the model of natural average distribution from a practical point of view. In this experiment, a comparison of performance tests of AODV, DSR and TORA is made by OPNET. From simulation results, it can be found that the performances of the three routing protocols are different and different protocols have different superiority in certain environment. These experimental results are provided as a basis for hardware implementation of wireless ad hoc networks.
  Lin Zhang , Zhengbang Liu , Kaili Rao and Ruchuan Wang
  In order to ensure the reliability and security of working environments under the open network environment dynamically,a fine-grain real-time and dynamic trust monitoring model is proposed which is based on behavior attribute factor, penalty factor of bad frequency, penalty factor of reputation, penalty factor of hazard-warning frequency, union-recommendation trust, etc. Through monitoring the behavior change in the context of service nodes, the calculating method about the behavior risk value is discussed which is based on the degree of damage about bad behaviors. This can provide convenience for making new judgment.When work is completed, the updating method about trust encouragement or punishment is given. Experiment results show that the new real-time trust monitoring model is accurate and effective.
  Na Li , Jia-Bo Wang , Yan-Ling Zhao , Lin Zhang , Xi-Bo Ma , Xiao-Fei Li , Jie Song , Xin Yang , Xiao-He Xiao , Jie Tian and Ting-Guo Kang
  Background: Hepatocytes damage is sometimes closely related to oxidative stress and reactive oxygen species which are the major contributors to lipopolysaccharide-induced liver injury. Emodin, the active natural product in rhubarb of hydroxyanthraquinone skeleton, has been reported of protective activity to liver tissue, whose mechanism is generally thought of antioxidation based on chemical reaction or indirect evidence. There is no visualized evidence proved the reactive oxygen species scavenging effect of emodin in vivo. Materials and Methods: The dynamic reactive oxygen species luminescent signal in mice injured by bacillus calmette guerin and lipopolysaccharide was monitored by using the optical molecular imaging approach. Results: The elevations of serum alanine aminotransferase and aspartate transaminase activities in bacillus calmette guerin/lipopolysaccharide-injured mice were reversed by emodin, indicating the protection of emodin to hepatocytes. And emodin significantly and dose-dependently attenuated the reactive oxygen species luminescent signal elicited by bacillus calmette guerin/lipopolysaccharide, indicating visually the in vivo reactive oxygen species scavenging effect of emodin. In addition, emodin significantly and dose-dependently elevated the activity of superoxide dismutase, content of reduced glutathione and total antioxidant capacity and meanwhile decreased the contents of hydrogen peroxide, lipid peroxides and malondialdehyde in livers of bacillus calmette guerin/lipopolysaccharide-injured mice. It could be attributed to the anti-oxidative effect of emodin which helps to maintain the reactive oxygen species balance in vivo. Conclusion: Emodin can protect liver against bacillus calmette guerin/lipopolysaccharide-induced injury and the mechanism includes reactive oxygen species scavenging effect and anti-lipid peroxidation at least.
  Xiao-Hong Wu , Ye Liu , Lin Zhang , Fei Li , Fei Wang , Li Cao , Xiao-Jun Yang and Jun-Hu Yao
  This experiment was conducted to investigate the effects of natural Vitamin E (Nat E Ac) level and duration of supplementation on growth performance, carcass traits, meat quality and α-tocopherol content as well as oxidation stability of plasma and breast meat (refrigerated at 4°C) of broilers. Cobb broilers (n = 315, 21 days old, female) were randomly assigned to 7 treatments (1 control and 6 experiment groups) with 5 replicates and 9 broilers per replicate. Control group was fed with basal diet (Nat E Ac:30 IU kg-1). For the experimental groups, a 2x3 factorial design was used with 2 Nat E Ac levels (Nat E Ac:60 and 120 mg kg-1) and 3 durations (7, 14 and 21 days prior to slaughter at 42 day). The broilers were in the finisher phase (22-42 days) during the treatment. Results showed that broilers fed with higher Nat E Ac levels or fed for a longer duration had significantly (p<0.05) increased percentage of breast and thigh meat, pH24h in breast, glutathione peroxidase in liver and α-tocopherol in plasma, liver and breast meat. Moreover, the broilers also exhibited notably (p<0.05) decreased drip loss and pH in breast meat as well as decreasing content of Malondialdehyde (MDA) in the plasma and breast meat refrigerated at 4°C for 0, 2, 4, 6 and 8 days. There was a significant (p<0.05) interaction between Nat E Ac level and feeding duration that affected the level of MDA in breast meat refrigerated at 4°C for 0, 2, 6 and 8 days. Nat E Ac level, feeding duration and the interaction of these two factors did not markedly (p>0.05) influence growth performance, breast meat color, percentage of eviscerated carcass and abdominal fat, total superoxide dismutase and glutathione peroxidase activities and antioxidation capacity in plasma. Higher Nat E Ac level or prolonged duration of feeding increased the percentages of breast and thigh meat, enhanced the water-holding capacity, the pH24h as well as α-tocopherol retention of breast meat and oxidative stability during refrigerated storage of breast meat thus enhancing the oxidative stability during refrigerated storage and improving breast meat quality.
  Yuanzhi Wang , Ke Zhang , Yali Zhang , Hui Wang , Fei Guo , Lin Zhang , Hui Zhang , Lijuan CaoBuyun , Cui , Chengyao Li , Li Yuan , Wanjiang Zhang , Ze Xu and Chuangfu Chen
  An outbreak of brucellosis occurred in students on field practice at sheep farm in 2005 at Shihezi, Xinjiang Province, the North-West of China. Five of 7 (71.4%) students were seropositive, showing titers ≥1:160 IU mL-1 in STAT and diagnosed as acute brucellosis with physical examination. To characterize Brucella isolates from the outbreak, the research including face to face investigation, Brucella isolation, multiple locus VNTR-16 analysis (MLVA-16) and genome sequencing were carried out. The investigation showed 42.5% (1,293/3,042) of ewes are sero-positive with RBPT and almost half of ewes aborted. Although, no bacteria were isolated from student blood samples, five individual colonies were isolated from aborted sheep fetuses and were identified as B. melitensis biovar 3 by conventional microbiological tests. MLVA-16 typing indicated that the isolates were clustered in the East Mediterranean with genotype 42. They were similar to wild strains from Guangdong in 2008 and Inner Mongolia in 1994 and 1995. They were most close to strain bru0261 from Pakistan student studying in Germany. Genome sequence and phylogenomic tree showed that pathogen in this study was close to Chinese wild and vaccine strains such as B. melitensis M28, M5-90. Researchers first report pathogens isolated in 1980 and 2005 are genotype 42 containing novel MLVA-16 patterns (1-5-3-13-2-2-3-2-4-20-8-8-4-3-7-7) compared to that both in China and other countries.
  Lin Zhang , Xijun Ou , Guoping Zhao and Xiaoming Ding
  Highly Efficient In Vitro Site-Specific Recombination System Based on Streptomyces Phage &phi/BT1 IntegraseThe Streptomyces phage φBT1 encodes a site-specific integrase of the large serine recombinase subfamily. In this report, the enzymatic activity of the φBT1 integrase was characterized in vitro. We showed that this integrase has efficient integration activity with substrate DNAs containing attB and attP sites, independent of DNA supercoiling or cofactors. Both intra- and intermolecular recombinations proceed with rapid kinetics. The recombination is highly specific, and no reactions are observed between pairs of sites including attB and attL, attB and attR, attP and attL, or attP and attR or between two identical att sequences; however, a low but significant frequency of excision recombination between attL and attR is observed in the presence of the φBT1 integrase alone. In addition, for efficient integration, the minimal sizes of attB and attP are 36 bp and 48 bp, respectively. This site-specific recombination system is efficient and simple to use; thus, it could have applications for the manipulation of DNA in vitro.
  Zhong- Lu You , Xiao Han , Peng Zhou and Lin Zhang
  A new Schiff-base ligand 2-bromo-4-chloro-6-(cyclopropyliminomethyl)phenol and its zinc(II) complex have been synthesized and characterized by elemental analyses, infrared spectroscopy, 1H NMR, 13C NMR, and single crystal X-ray determinations. The ligand and the complex crystallized in the space groups Pnma and P21/c, respectively. In the complex, the Zn atom is four-coordinate tetrahedral coordination with two imine N and two phenolate O atoms from two Schiff-base ligands. The bond lengths related to the donor atoms in the complex are different from those in the ligand. The coordination of the ligand to the zinc also was supported by IR spectra.
  Xiao-Zhi He , Bao-Yan Zhang , Wen-Wen Ma , Lin Zhang and Qiang- Mu
  In this work the new-style nematic monomer M1, chiral crosslinking reagent MC and a series of new side-chain cholesteric liquid crystalline elastomers derived from M1 and MC were prepared. The effect of the content of the chiral crosslinking unit on phase behaviour of the elastomers has been discussed. Polymer P1 showed nematic phase, P2-P7 showed cholesteric phase, P3 formed Grandjean texture in the heating cycle and turned out a blue Grandjean texture in the cooling cycle, P2-P3 with less than 6 mol% of chiral crosslinking agent gave rise to selective reflection. The elastomers containing less than 15 mol% of the crosslinking units displayed elasticity, reversible phase transition and high thermal stability. Experimental results demonstrated that the glass transition temperatures reduced first and then increased, and the isotropisation temperatures and the mesophase temperature ranges decreased with increasing content of crosslinking unit.
  Sean P. Garrison , John R. Jeffers , Chunying Yang , Jonas A. Nilsson , Mark A. Hall , Jerold E. Rehg , Wen Yue , Jian Yu , Lin Zhang , Mihaela Onciu , Jeffery T. Sample , John L. Cleveland and Gerard P. Zambetti
  The p53 tumor suppressor pathway limits oncogenesis by inducing cell cycle arrest or apoptosis. A key p53 target gene is PUMA, which encodes a BH3-only proapoptotic protein. Here we demonstrate that Puma deletion in the Eµ-Myc mouse model of Burkitt lymphoma accelerates lymphomagenesis and that ~75% of Eµ-Myc lymphomas naturally select against Puma protein expression. Furthermore, approximately 40% of primary human Burkitt lymphomas fail to express detectable levels of PUMA and in some tumors this is associated with DNA methylation. Burkitt lymphoma cell lines phenocopy the primary tumors with respect to DNA methylation and diminished PUMA expression, which can be reactivated following inhibition of DNA methyltransferases. These findings establish that PUMA is silenced in human malignancies, and they suggest PUMA as a target for the development of novel chemotherapeutics.
  Lin Zhang , Cheang Sao Khoo , Sundar Rao Koyyalamudi , Nuria de Pedro and Narsimha Reddy
  Background and Objective: Medicinal herbs offer an important traditional way to prevent and cure several diseases such as cardiovascular disease, chronic inflammation and cancer as they contain bioactive compounds including those with antioxidant, immunomodulatory and anticancer activities. The purpose of this study was to determine biological activities of organics from hot water extracts of medicinal herbs and to obtain the correlation of activities with polyphenol contents. Materials and Methods: In this study, 16 herbs were selected based on their traditional medicinal uses and obtained their hot water extracts. Ethanol soluble organic molecules were separated from these extracts and their antioxidant, immunomodulatory and anticancer activities were assessed. Antioxidant activities were evaluated using DPPH, ABTS•+ scavenging methods and ferric ion reducing assay. Total phenolic and total flavonoid contents of these extracts were estimated based on the Folin-Ciocalteu and aluminium chloride colorimetric methods. The immunomodulatory properties of the herbs were determined on the basis of their ability to inhibit NO and TNF-α production in LPS induced RAW 264.7 macrophages. Cell viabilities were determined using MTT assay. The anticancer activities were measured against five human cancer cell lines. All data was analysed using one-way ANOVA and Duncan’s multiple range methods. Results: Organic molecules extracted from Alpinae officinarum (A. officinarum), Artemisia annua, Cynanchum paniculatum, Lobelia chinensis (L. chinensis), Spatholobus suberectus (S. suberectus), Xanthium sibiricum and Amauroderma rugosum (A. rugosum) have exhibited significant antioxidant activities and considerably inhibited the production of NO and TNF-α. Seven herbal extracts out of sixteen herbs studied showed highly significant anticancer activity against MCF7. The extract from Rabdosia rubescens displayed significant anticancer activities against three cancer cell lines. Observed biological activities of the extracts showed good correlation with their flavonoid contents. Conclusion: Extracts from Akebia quinata, A. officinarum, Artemisia scoparia, L. chinensis, S. suberectus and A. rugosum exhibited significant biological activities with large quantities of polyphenols. These herbs are potential candidates for the isolation of novel anticancer agents.
  Lin Zhang , Cheang Soo Khoo , Sundar Rao Koyyalamudi , Nuria de Pedro and Narsimha Reddy
  Background and Objective: It is evident from the scientific literature that plant and mushroom polysaccharides possess significant antioxidant, immunomodulatory and anticancer activities. Sixteen traditional Chinese anticancer herbs were evaluated for the immunomodulatory and anticancer potential of their polysaccharides. This study also aimed to correlate the bioactivities of these polysaccharides with their monosaccharide composition and to identify the best herbs for further detailed studies. Methods: Polysaccharides were extracted from the selected traditional Chinese medicinal (TCM) herbs and their biological activities examined. The antioxidant activities were examined using DPPH scavenging, ABTS∙+ scavenging and iron chelating assays. The immunomodulatory properties of the polysaccharides were determined by evaluating their capacity to activate mouse macrophages (RAW 264.7) to produce the cytokines, namely, interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). The anticancer activities of the polysaccharides were determined against five human cancer cell lines. Cell viabilities were evaluated by the MTT test to assess the toxicities of these polysaccharides. The total sugar content and the monosaccharide composition of the polysaccharides were determined with a view to correlate their bioactivities with chemical constituents. All data were analyzed using ANOVA and Duncan’s multiple range methods. Results: The polysaccharides isolated from Artemisia annua L., Lobelia chinensis Lour, Amauroderma rugosum (Blume and T. nees), Artemisia scoparia Waldst. and Kit, Artemisia vulgaris L., Curcuma aromatic Salisb, Rheum palmatum L. and Cyperusrotundus Blanco showed significant anticancer, immunomodulatory and antioxidant activities with low toxicity. The results suggested that the polysaccharides extracted from A. annua, L. chinensis, A. rugosum and S. suberectus have strong potential for immunotherapy and hence suitable candidates for cancer treatment. Conclusion: Polysaccharides from several herbs were found to exhibit significant antioxidant, immunostimulatory and anticancer activities. The results demonstrated that, A. annua, L. chinensis and A. rugosum and S. suberectus are highly suitable herbs for the discovery of anticancer polysaccharides.
  Lin Zhang , Narsimha Reddy , Cheang Soo Khoo , Sundar Rao Koyyalamudi and Christopher E. Jones
  Background and Objective: Lobelia chinensis Lour is an important anticancer herb used in traditional Chinese medicine. Many botanical polysaccharides are known to exhibit immunomodulatory and anticancer activities. This research aimed to analyze the L. chinensis polysaccharides (LCPs) for their biological activities relevant to their anticancer function. Materials and Methods: Water-soluble LCPs were extracted and purified using size-exclusion chromatography to obtain two dominant polysaccharides, LCP-1 and LCP-2 having molecular masses of 1899 kDa and 5.3 kDa, respectively. The antioxidant potentials of the isolated polysaccharides were evaluated by measuring radical scavenging activities against DPPH (2,2-diphenyl-1-picrylhydrazyl radical), ABTS∙+(2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid radical) and OH(hydroxyl radical). Immunostimulatory activities of LCP-1 and LCP-2 were measured using mouse macrophages. Structure of the most active fraction (LCP-2) was determined using FT-IR and NMR spectroscopic techniques. Results: Two isolated polysaccharide fractions displayed significant antioxidant activities and stimulated the production of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), although LCP-2 is more effective. Detailed structural characterization by FT-IR and NMR was undertaken for the most active fraction (LCP-2) and confirmed that LCP-2 is(2,1)-β-fructan. Conclusion: The results suggested that the polysaccharides isolated from Lobelia chinensis Lour are potential candidates for immune-chemotherapy and suitable for the treatment of cancer.
  Ka Chen , Yizeng Tu , Yongjun Zhang , Harry C. Blair , Lin Zhang and Chuanyue Wu
  Resistance to apoptosis is a hallmark of cancer cells. We report here that PINCH-1, a cytoplasmic component of cell-extracellular matrix adhesions, is required for protection of multiple types of cancer cells from apoptosis. Furthermore, using HT-1080 fibrosarcoma cells as a model system, we have investigated the signaling pathway through which PINCH-1 contributes to apoptosis resistance. Loss of PINCH-1 markedly increases the level of Bim and promotes Bim translocation to mitochondria, resulting in activation of the intrinsic apoptosis pathway. Depletion of Bim completely blocked apoptosis induced by the loss of PINCH-1. Thus, PINCH-1 contributes to apoptosis resistance through suppression of Bim. Mechanistically, PINCH-1 suppresses Bim not only transcriptionally but also post-transcriptionally. PINCH-1 promotes activating phosphorylation of Src family kinase and ERK1/2. Consistent with this, ERK1/2-mediated Ser69 phosphorylation of Bim, a key signal for turnover of Bim, is suppressed by the removal of PINCH-1. Our results demonstrate a strong dependence of multiple types of apoptosis-resistant cancer cells on PINCH-1 and provide new insights into the molecular mechanism by which cancer cells are protected from apoptosis.

  Wenjing Luo , Jinyi Liu , Jingxia Li , Dongyun Zhang , Mingchao Liu , James K. Addo , Shivaputra Patil , Lin Zhang , Jian Yu , John K. Buolamwini , Jingyuan Chen and Chuanshu Huang
  p53, one of the most commonly mutated genes in human cancers, is thought to be associated with cancer development. Hence, screening and identifying natural or synthetic compounds with anti-cancer activity via p53-independent pathway is one of the most challenging tasks for scientists in this field. Compound JKA97 (methoxy-1-styryl-9H-pyrid-[3,4-b]-indole) is a small molecule synthetic anti-cancer agent, with unknown mechanism(s). In this study we have demonstrated that the anti-cancer activity of JKA97 is associated with apoptotic induction via p53-independent mechanisms. We found that co-incubation of human colon cancer HCT116 cells with JKA97 inhibited HCT116 cell anchorage-independent growth in vitro and tumorigenicity in nude mice and also induced a cell apoptotic response, both in the cell culture model and in a tumorigenesis nude mouse model. Further studies showed that JKA97-induced apoptosis was dramatically impaired in Bax knock-out (Bax-/-) HCT116 cells, whereas the knock-out of p53 or PUMA did not show any inhibitory effects. The p53-independent apoptotic induction by JKA97 was confirmed in other colon cancer and hepatocarcinoma cell lines. In addition, our results showed an induction of Bax translocation and cytochrome c release from the mitochondria to the cytosol in HCT116 cells, demonstrating that the compound induces apoptosis through a Bax-initiated mitochondria-dependent pathway. These studies provide a molecular basis for the therapeutic application of JKA97 against human cancers with p53 mutations.
  Dawei Jiang , Wenqian Chen , Jianmin Xiao , Ruiwu Wang , Huihui Kong , Peter P. Jones , Lin Zhang , Bradley Fruen and S. R. Wayne Chen
  Naturally occurring mutations in the skeletal muscle Ca2+ release channel/ryanodine receptor RyR1 are linked to malignant hyperthermia (MH), a life-threatening complication of general anesthesia. Although it has long been recognized that MH results from uncontrolled or spontaneous Ca2+ release from the sarcoplasmic reticulum, how MH RyR1 mutations render the sarcoplasmic reticulum susceptible to volatile anesthetic-induced spontaneous Ca2+ release is unclear. Here we investigated the impact of the porcine MH mutation, R615C, the human equivalent of which also causes MH, on the intrinsic properties of the RyR1 channel and the propensity for spontaneous Ca2+ release during store Ca2+ overload, a process we refer to as store overload-induced Ca2+ release (SOICR). Single channel analyses revealed that the R615C mutation markedly enhanced the luminal Ca2+ activation of RyR1. Moreover, HEK293 cells expressing the R615C mutant displayed a reduced threshold for SOICR compared with cells expressing wild type RyR1. Furthermore, the MH-triggering agent, halothane, potentiated the response of RyR1 to luminal Ca2+ and SOICR. Conversely, dantrolene, an effective treatment for MH, suppressed SOICR in HEK293 cells expressing the R615C mutant, but not in cells expressing an RyR2 mutant. These data suggest that the R615C mutation confers MH susceptibility by reducing the threshold for luminal Ca2+ activation and SOICR, whereas volatile anesthetics trigger MH by further reducing the threshold, and dantrolene suppresses MH by increasing the SOICR threshold. Together, our data support a view in which altered luminal Ca2+ regulation of RyR1 represents a primary causal mechanism of MH.
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