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Articles by Lin Guo
Total Records ( 2 ) for Lin Guo
  Yu-Peng Ren , Xiao-Yu Wang , Qi-Gui Yan , Di-Shi Chen , Yu Cheng , Li Wan , Lei Ma , Qi Zhang , Lin Guo , Zhi-Yong Xie and Wan-Zhu Guo
  This study examined the recombinant Attenuated Salmonella Choleraesuis C500 containing M and N gene of PEDV whether can induce a systemic immune responses in mice. After identified by enzyme analysis and nucleotide sequencing test, the constructed recombinant plasmid, designated as pIRES-M-N was transfected into Vero cells. The transient expression protein was detected by indirect Immunofluorescence Assay (IFA) and Western-blotting. Result showed that pIRES-M-N can be expressed correctly in Vero cells. Then, the recombinant plasmid electroporated into S. choleraesuis C500 and the recombinant bacteria was proved to be stabile in vitro. The immunogenicities of this recombinant attenuated salmonella constructs were firstly investigated in a mouse mode. Specific anti-PEDV IgG antibodies and IFN-γ, IL-4 of cytokine were detected. The result showed that the recombinant Attenuated Salmonella could induce higher persistence specific hummune responses in mouse by contrast with naked DNA group after 42 days (p<0.05). It also, stimulated a balanced Th1/Th2 immune response. That provided the clinical basic data and theoretical basis for success of the attenuated salmonella vaccine development.
  Wang Hu , Haiyan Zhao , Liankun Shan , Li Song , Hui Cao , Zhou Yang , Zihui Cheng , Chunzhu Yan , Sijin Li , Huai Yang and Lin Guo
  A magnetite (Fe3O4) nanoparticle/chiral nematic liquid crystal (N*-LC) composite was prepared and filled into a planar treated cell. The Fe3O4 nanoparticles had been modified by oleic acid so that they could be better dispersed in the composite. When a magnetic field was scanned on the outer surface of the cell locally, Fe3O4 nanoparticles moved towards the inner surface of the cell correspondingly, and the black expected information was displayed. When the magnet was applied to the opposite outer surface, the information was erased. After polymer network walls were prepared in the composite, the resolution of the information displayed increased. Then, through the formation of hydrogen bonds between the nanoparticles and chiral pyridine compound (CPC) doped in the composite, the pitch length of the N*-LC could be adjusted by altering the intensity of the applied magnetic field. The composite doped with CPC could potentially be used as a material for a type of reflective colour paper with magnetically controllable characteristics.
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