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Articles by Li Wen
Total Records ( 3 ) for Li Wen
  Xu Chang-Biao and Li Wen
  In LTE-Advanced systems, as for the problems of the deficiency of higher system blocking probability and lower customer satisfaction in static CoMP frequency management Scheme, a dynamic CoMP frequency management scheme is proposed, this scheme include two parts which establishes a transitional frequency band and Paired In-Out Scheme (PIOS). System-level simulation results show that, the transitional frequency band can reduce the system blocking probability effectively; And the Paired In-Out Scheme can not only further reduce the blocking probability, but also improve the system throughput and the level of Quality of Experience, effectively.
  Mohamed Abdalla Elsiddig Mohamed , Cao Yangchun , Bello Musa Bodinga , Zheng Lixin , Yang Zekun , Liu Lihui , Junhu Yao , Rab Nawaz Soomro , Imtiaz Hussain Raja Abbasi and Li Wen
  Background and Objective: Numerous in vitro studies have showed that exogenous fibrolytic enzyme can enhance fiber degradation of roughage. The aim of this study was to evaluate the effect of an Exogenous Fibrolytic Enzymes (EFE) product applied to a total mixed ration on ruminal fermentation, gas production and nutrient digestion in vitro whole digestive tract. Materials and Methods: The total mixed ration contained 55% concentrates, 22.5% corn silage and 22.5% alfalfa hay on a dry matter basis. The EFE multi enzyme feed additive powder produced from Ruminococcus flavefaciens was used in this study. Four levels of the EFE (0.0, 0.4, 0.8 and 1.2 g) were used E0, E2, E4 and E6, respectively, these levels of EFE were added directly into 20 g of diet in nylon bags and incubated in the RUSITEC for 48 h in vitro fermentation. Statistical analyses of continuous data were performed by use of SAS with repeated measures or Tukey’s test.Results: Results of the rumen experiment showed no significant difference (p>0.05) in pH-values, dry matter, organic matter digestibility, crude protein digestibility, gas production, CH4 and total volatile fatty acids production among all treatments in RUSITEC by using different EFE levels. The digestibility of neutral detergent fibre, acid detergent fiber and NH3-N concentrations were increased (p<0.05) with increasing EFE levels. The in vitro abomasum digestibility and total volatile fatty acids were unchanged (p>0.05). NH3-N concentrations were also increased (p<0.05) with increasing EFE levels. Moreover, in the in vitro abomasum experiment, the neutral detergent fibre digestibility and acid detergent fiber digestibility was increased (p<0.05) with increasing EFE levels. Conclusion: The results indicate that using high doses of EFE (VTR®) containing Ruminococcus flavefaciens in TMR (45:55) could improve the neutral detergent fibre digestibility and acid detergent fiber digestibility in rumen and small intestine.
  CHEN Ge-fei , ZHANG Yun-long , LIN Sen-zhu , LI Wen and MENG Qing
  Extraction of high molecular weight genomic DNA (HMW-gDNA) from spider organs and construction of an electroelution system for obtaining HMW-gDNA efficiently are described. We improved the CTAB method to isolate genomic DNA from spider pectoral muscle tissues, constructed a novel electroelution system with the use of 2-mL centrifuge tube and dialysis membrane, and utilized this self-constructed electroelution system to recover target HMW-gDNA from agarose gels. The HMW-gDNA extracted using the improved CTAB method could be over 48.5 kb in size. By trapping the HMW-gDNA with dialysis membrane, the electroelution system could recover target HMW-gDNA from agarose gels rapidly and without affecting the integrity of HMW-gDNA. The recovery rate was over 75% with the OD260/OD280 ranging from 1.8 to 2.0. The results demonstrate that the improved CTAB method can be used to extract spider HMW-gDNA, and the electroelution system is a general method for recovering target HMW-gDNA from agarose gels with low cost, simplicity, high efficiency and good practicability.
 
 
 
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