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Articles by Li Wang
Total Records ( 11 ) for Li Wang
  Xuewen Huang , Li Wang , Lijian Yang and Alexandra N. Kravchenko
  Crop yields are highly variable spatially and temporally as a result of complex interactions among topography, weather conditions, and management practices. The objective of this study was to analyze the effects of management practices on the relationship between crop yields and precipitation and crop yields and topography using 10 yr of yield data from a long-term corn (Zea mays L.)-soybean [Glycine max (L.) Merr.]-wheat (Triticum aestivum L.) rotation experiment in southwest Michigan. The four agronomic treatments studied were chisel plowed with conventional chemical inputs (CT), no-till with conventional chemical inputs (NT), chisel plowed with low chemical input and a winter leguminous cover crop (CTL), and organic-based chisel plowed with a winter leguminous cover crop (CTO). A nonparametric (spline) regression was used to characterize the relationship between the maximal yields, as characteristics of yield potential, and a wetness index (WI), as an integrative characteristic of topographical features related to water flow, and to compare the yield differences between the treatments across a range of the WI values. Variability of yields in NT and CTO systems was better explained by precipitation in early spring and during pollination and grain fill than that in CT and CTL. No-till and CTL tended to produce higher maximal yields than CT at the summit/steep-sloped areas (lower WI), while at intermediate and high WI levels the differences between them were inconsistent. The CTL often produced higher maximal yields than CTO at low and intermediate WI values, while the difference between them was mostly not significant at high WI levels (depression areas). The nonparametric spline regression algorithm used in the study was robust and efficient in comparing the yield differences between treatments across a range of WI values.
  Yuexiang Yang , Yingcheng Xu and Li Wang
  This study deals with the Multi Depot Heterogeneous Vehicle Routing Problem with Time Windows (MDHVRPTW) which is complex and still not resolved well. The objective is to determine the best fleet composition as well as the set of routes that minimize the total cost with known demands. To solve the problem, mathematical model of the MDHVRPTW is constructed and an improved variable neighborhood search algorithm is proposed. In the algorithm, the hybrid operators of insert and exchange are used to achieve the shaking process and the later optimization process is presented to improve the solution space, the best-improvement strategy is adopted which make the algorithm can achieve a better balance in the solution quality and running time. The idea of simulated annealing is introduced to take control of the acceptance of new solutions. The developed algorithm was tested in benchmark instances. The results obtained are quite competitive with those found in the literature and new improved solutions are reported. And finally the proposed model and algorithm is applied to the large water project in China to solve the allocation of vehicles and routes, it demonstrates that the systematic method is effective and feasible.
  Bingjie Li , Li Wang , Xiaodan Xu , Zhiliang Wang , Bosen Li and Jiwei Liu
  Aiming at the issue that air is uniformity controlled in a special pipeline flow, With the concentration on the control of a pipeline flow, a model of viscous incompressible fluid turbulence is presented. according to comparing numerical simulation with the measured data verify the mathematical model built with the actual agreement. A new way was presented to transforming distributed parameter models into lumped parameter control system. Based on the control system model above, optimal control of air velocity distribution is researched by application of state feedback linearization control algorithm. The effectiveness of the algorithm is verified by simulation. The results showed that the control algorithm can better control the controlled object. This method has shed some light on the design of pipeline flow control system in the actual system.
  Haiqing Yu , Chun Zhang , Zhiqiang Mei , Li Wang , Juan Li , Chenggang Duan , Xiaoyan Liu , Shu Gong , Lin Gan , Zhonghua Tao , Fuli Yao , Ye Yang , Chunyan Duan , Youping Liu , Chunyan Zhang , Chuanning Chen , Xiaoli Zheng , Hongxian Zhao , Jiyan Cheng , Xiaojun Tang , Yong Zhang , Yingxi Zhang and Junjiang Fu
  As a traditional medicinal herb in China, Penthorum chinense Push from Gulin county is regarded as genuine regional drug with better clinical effects. To discriminate the geographical origin of six P. chinense samples cultivated in three provinces, Randomly Amplified Polymorphic DNA (RAPD) analysis was carried out with an improved method to increase the resolution and production using 10 mer-random primers. Similarity index was ranged from 0.61 to 0.97, which demonstrated that samples from different localities displayed similar band patterns. However, based on the analysis of selected 13 primers, primers SBS-I9, SBS-I20 and SBS-Q9 produced distinguishable bands among Sichuan, Yunnan and Hubei P. chinense. T-test of mean S.I. values of six accessions demonstrated the significant differences between Luzhou or Sichuan samples and others. Cluster analysis indicated that cultivars with close geographic distributions were clustered together consequently. This suggested that there are RAPD site variations and local specialized genotypes among the six samples. The results indicated that RAPD analysis is effective in distinguishing the geographical origin of genuine regional drug P. chinense grown in Luhzou, Sichuan. The approach is a valuable tool to authenticate other morphologically similar herbal medicinal materials.
  Li Wang , Hua-Neng Xu , Huiyuan Yao and Hui Zhang
  The water, ethanol, and methanol extracts of Vaccinium Bracteatum Thunb. leaves (VBTL) were investigated for their potential antioxidant activity. The total phenolic content was determined, and the antioxidant activity of the extracts was assessed by 1, 1–diphenyl–2–picrylhydrazyl (DPPH) radical scavenging analysis. Five phenolic compounds including quercetin, kaempferol, luteolin, apigenin, and chrysin, were identified in the ethanol extract by high pressure liquid chromatography (HPLC).
  Fei Liu , Babangida Lawal Yusuf , Jianli Zhong , Lei Feng , Yong He and Li Wang
  Visible and near infrared spectroscopy was investigated to identify the varieties of rice vinegars based on back propagation neural network and least squares-support vector machine. Five varieties of rice vinegars were prepared. Partial least squares discriminant analysis was implemented for calibration and extraction of partial least squares factors. The factors were used as inputs of back propagation neural network and least squares-support vector machine. Finally, back propagation neural network and least squares-support vector machine models were achieved. The threshold value of prediction was set as ±0.1. An excellent precision and recognition ratio of 100% was achieved by both methods. Simultaneously, certain effective wavelengths were proposed by x-loading weights and regression coefficients. The performance of effective wavelengths was validated and an acceptable result was achieved. The results indicated that visible and near infrared spectroscopy could be used as a rapid and high precision method for the identification of different varieties of rice vinegars.
  Ruiling Qin , Yaocheng Chu , Junfeng Lian , Zhanyun Xu , Chunhong Li , Li Wang and Guohui Li
  The objective of the present study was to evaluate the effects of Lycium barbarum Polysaccharide (LBP) on immune responses in vaccinated chickens. A total of 600 Hy-Line Brown chickens aged 15 days old were randomly divided into four groups with three replicates per group and fifty chickens per replicate and all the chickens were injected with Newcastle Disease (ND) vaccine. Three experimental groups of chickens were injected with LBP 20, 10 and 5 mg kg-1 (LBPH, LBPM and LBPL) and the control group were injected with equal dose normal saline (0.09% NaCl), respectively per day for 7 days. On the 7, 14, 21, 28, 35 and 42 days after vaccination, ten chickens were sampled randomly from each group and the serum was seperated for the determination of NDV-HI antibody titers by Micro Method. On day 10, 20, 30, 40 and 50 after vaccination, blood samples from 5 chickens per group were collected to separate lymphocyte and determine the peripheral blood T lymphocyte proliferation with Methyl Thiazolyl Tetrazolium (MTT) Method. The content of CD4+ and CD8+ T cells were tested by using flow cytometry with Double Color Staining Method. The IL-2 levels were determined by ELISA Method and the whole body, bursa of fabricius and thymus were weighted for calculation of immune organ index. The results showed that LBP (10 and 20 mg kg-1) could significantly raised the ratio of CD4+ and CD8+T lymphocyte (p<0.01) and the production of IL-2. It also significantly enhanced the ND antibody titers, promoted the proliferation of peripheral blood T lymphocyte and increased immune organ indexes (p<0.01). These results indicated that LBP had significant immunoregulation functions of ND vaccine in chickens.
  Li Wang and Yang Xiang
  This study is focus on the tangential contact characteristics of a single hemispherical asperity when the asperity deformed in the stages of normal elastic-plastic deformation by the finite element method. The normal elastic-plastic contact characteristics of a single asperity contacted with a rigid flat has been studied by some researchers, but the tangential contact characteristics of them is still missing in the detail. The single asperity finite element model is a 3D finite element solid model in the study of the tangential contact characteristics, it is different the plane finite element model in the study of the normal contact characteristics. The local mesh refinement method is applied to mesh the asperity model, within the elastic deformation of the asperity, the normal contact load and area in the finite element analysis have been compared with the Hertz contact theory, the difference is less than 5%, the asperity 3D finite element model has been verified. For tangential contact analysis, a normal displacement is applied at the pilot node of the rigid flat, the asperity will deformation from the elastic to plastic range. Then hold the deformation state, a tangential force is again applied to the pilot node , the rigid flat will move on the surface of the asperity in the range of the micro-displacement state. Under the different normal displacement, the results shows that linear relationship can be obtained between tangential force and tangential micro-displacement, in the range of about , tangent modulus has not obviously effect on the tangential contact stiffness; In the range of about , tangential contact stiffness increasing with the tangent modulus increasing.
  Zi-Liang Wang , Xiao-Peng Xu , Bai-Liang He , Shao-Ping Weng , Jia Xiao , Li Wang , Ting Lin , Xi Liu , Qing Wang , Xiao-Qiang Yu and Jian-Guo He
  Infectious spleen and kidney necrosis virus (ISKNV) causes a pandemic and serious disease in fish. Infection by ISKNV causes epidermal lesions, in which petechial hemorrhages and abdominal edema are prominent features. ISKNV ORF48R contains a domain similar to that of the platelet-derived growth factor and vascular endothelial growth factor (VEGF) families of proteins. ISKNV ORF48R showed higher similarity to the VEGFs encoded by Megalocytivirus and Parapoxvirus than to those encoded in fish and mammals. We used zebrafish as a model and constructed a recombinant plasmid containing the DNA sequence of ISKNV ORF48R to study ISKNV infection. The plasmid was microinjected into zebrafish embryos at the one-cell stage. Overexpression of the ISKNV ORF48R gene results in pericardial edema and dilation at the tail region of zebrafish embryos, suggesting that ISKNV ORF48R induces vascular permeability. ISKNV ORF48R is also able to stimulate a striking expression of flk1 in the zebrafish dorsal aorta and the axial vein. Furthermore, ISKNV ORF48R, while cooperating with zebrafish VEGF121, can stimulate more striking expression of flk1 than can either ISKNV ORF48R or zebrafish VEGF121 alone. However, decreased expression of FLK-1 by gene knockdown results in the disappearance of pericardial edema and dilation at the tail region of zebrafish embryos induced by overexpression of ISKNV ORF48R in the early stages of embryonic development.
  Li Wang , Sharmistha Pal and Said Sif
  The proper epigenetic modification of chromatin by protein arginine methyltransferases (PRMTs) is crucial for normal cell growth and health. The human SWI/SNF-associated PRMT5 is involved in the transcriptional repression of target genes by directly methylating H3R8 and H4R3. To further understand the impact of PRMT5-mediated histone methylation on cancer, we analyzed its expression in normal and transformed human B lymphocytes. Our findings reveal that PRMT5 protein levels are enhanced in various human lymphoid cancer cells, including transformed chronic lymphocytic leukemia (B-CLL) cell lines. PRMT5 overexpression is caused by the altered expression of the PRMT5-specific microRNAs 19a, 25, 32, 92, 92b, and 96 and results in the increased global symmetric methylation of H3R8 and H4R3. An evaluation of both epigenetic marks at PRMT5 target genes such as RB1 (p105), RBL1 (p107), and RBL2 (p130) showed that promoters H3R8 and H4R3 are hypermethylated, which in turn triggers pocket protein transcriptional repression. Furthermore, reducing PRMT5 expression in WaC3CD5 B-CLL cells abolishes H3R8 and H4R3 hypermethylation, restores RBL2 expression, and inhibits cancer cell proliferation. These results indicate that PRMT5 overexpression epigenetically alters the transcription of key tumor suppressor genes and suggest a causal role of the elevated symmetric methylation of H3R8 and H4R3 at the RBL2 promoter in transformed B-lymphocyte pathology.
  Jiandong Mao , Dengxin Hua , Yufeng Wang , Fei Gao and Li Wang
  An ultraviolet rotational Raman lidar (RRL) system at an eye-safe wavelength of 354.7 nm is demonstrated for profiling the vertical temperature of the atmospheric layer. Two sets of narrow-band interference filters, which central wavelengths are located at 353.9 and 352.5 nm, respectively, are employed to block the elastic Mie- and Rayleigh-scattering signals and to separate two required rotational Raman signals for temperature retrieval. Experiments were carried out for verifying the feasibility of the prototype of the ultraviolet RRL from May to November in 2008, and the system calibrations were performed with the radiosonde data which were obtain from the local meteorological bureau, and good agreements were got in the experiment results. The observation results show that a statistical temperature error of less then 1 K was obtained up to the heights of 2.5 km for nighttime and 2.0 km for daytime with 300 mJ laser energy and not, vert, similar8 min observation time.
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