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by
Li Qiu |
Total Records (
2 ) for
Li Qiu |
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Li Qiu
,
Ton Dietz
and
Fu Rong
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With the emergence of the mass collaboration paradigm along
with the rapid development of Internet Communication Technologies (ICTs), the
wide acceptance of mass collaboration is inevitable with its irresistible advantages
in transmitting, sharing and integrating knowledge among participants. In this
context, initiatives in creating practical and sustainable knowledge networks
are highly desired. As an explorative initiative supported by the International
Geographical Union (IGU), www.oursus.org with its Chinese and English-version
websites, a Global Sustainable Development Cities Information Network (IGU-GSCIN),
was put forward in 2010 adopting a so-called rainbow knowledge category approach,
task modules for different role participants and the most popular ICTs, such
as social networks and location-based systems. Taking an action research approach,
we believe that it is quite possible for this kind of website to develop as
a multi-dimensional knowledge network for global sustainable cities which includes
products with sustainable features provided by enterprises, cases and problem
observation by NGOs and experts, experience exchange by individuals, campaigns
by volunteers, etc. |
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Li Qiu
,
Chao Sun
,
Hu Yang
,
Chunli Li
,
Zhang Feng
,
Yahui Zhao
,
Xiaoye Liu
,
Rui Wang
,
Min Duan
,
Xinglong Wang
and
Zengqi Yang
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This study aims to establish a rapid and sensitive multiplex PCR Method for Salmonella detection and the differentiation of virulent strains. Four sets of primers that target hisJ, spvR and fliC were designed and the PCR reaction conditions were then optimized. Based on the detection of reference Salmonella strains and other reference bacterial genera, the PCR Method was able to detect hisJ, spvR, fliC-c and fliC-i in Salmonella. The minimum detectable DNA concentration of the method is 100 pg. The results using the reference strains were consistent with previous research. In addition, this method can also be used in identifying S. typhimurium and S. arizona. Researchers successfully developed a convenient and useful multiplex PCR Method for detecting Salmonella and monitoring virulent strains. |
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