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Articles by Li Bi-Chun
Total Records ( 4 ) for Li Bi-Chun
  Wu Tian-Wen , Xu Gui-Jiang , Pan Yu-Lai , Xie Xi-Ping , Li Bi-Chun and Wu Xin-Sheng
  The genetic diversity and phylogenetic relationship of 7 China rabbit populations were investigated with 15 microsatellite. The results showed that: The value of the average expected heterozygosity (He range from 0.820±0.012-0.675±0.031) Polymorphic Information Content (PIC range from 0.796±0.016-0.625±0.033) and the mean effective number of alleles (Ne = 6.625±0.498) of the seven rabbit populations were high, which indicated that polymorphisms and genetic diversity of genes were abundant. The range of FST for the whole population was from 0.041 (6L3F8) -0.195 (Sat8). The mean FST was 0.099±0.010; the average of total inbreeding coeffient (FIT) was -0.004±0.052; the mean inbreeding coeffient among populations (FIS) was -0.114±0.050. The dendrogram by Unweighed Pair-Group Method with Arithmetic averaging (UPGMA) based on Nei’s genetic distance and Reynolds' genetic distance was similar. Seven populations were clustered into 4 groups. The Germany Angora Rabbit, American Rex Rabbit and Wan-line Angora Rabbit belonged to the first group; the New Zealand White Rabbit and Zika Rabbit were included in the second group; the Fujian Yellow Rabbit and Fujian Black Rabbit were clustered separately. The results suggested that the 15 microsatellite loci were effective markers for analysis of genetic relationships among rabbit populations.
  Tian Zhi-Quan , Wu Xiao-Wei , Sun Min , Yang Hai-Yan , Chang Guo-Bing , Ren Li-Wei and Li Bi-Chun
  The aim of this study was to examine Single Nucleotide Polymorphism (SNP) of resistant sequence in Mx gene in chicken with a mismatched Polymerase Chain Reaction-restriction Fragment Length Polymorphism (PCR-RFLP) method and thus, the differences of frequency on sequence A2032G in Mx gene in different breeds were detected. The results showed that three genotypes were found on A2032G in 15 breeds of chicken, which were AA, AG, GG and AA genotype was not found in Luyuan, Langshan chicken and Zhongyuan Game chicken, as GG genotype did in Chahua chicken and Red Jungle Fowl. The average frequency of resistance gene (A) and sensitive gene (G) was 0.350 and 0.650, respectively. However, the frequency of A alleles had a wide rangeability from 0.034-0.984 and the results of χ2-test indicated that Mx gene in all populations was in Hardy-Weinberg equilibrium.
  LI Bi-Chun , Tian Zhi-Quan , Chen Hao , Sun Min , Yang Hai-Yan , Ren Li-Wei , Qin Yu-Rong , Xu Feng and Chen Guo-Hong
  The effect of Dimethylsulphoxide (DMSO), Glycerol (GLY) and Propanedo (PROH) cryoprotectants, each at (10, 20 and 30%) concentration on the preservation of mouse and cattle skin tissue, was investigated. And then, the toxicity test was performed by exposing the skin tissue to DMSO, GLY, PROH, EG without freezing. Tissues were checked by capacity explants. The results indicated that mouse skin tissue frozen in 10% GLY and cattle ear tissue frozen in 20% GLY medium with dry-ice yielded significantly (p<0.05) higher percents (33.3 and 71.4%) of fibroblast than tissue frozen in either concentration of GLY or cryoprotectants. In the toxicity test, mouse skin tissues exposed to GLY and equilibrated at 4°C resulted in higher percent of average explants (41.1%) than that was exposed to PRON and DMSO (average explants 38.3 and 36.5%, respectively), while cattle tissues showed higher toxicity, when exposed to PROH and EG and equilibrated at 4°C (average explants 39.9 and 44.2%, respectively). The present study recommended that 10% GLY with dry-ice freezing was the effective cryoprotectant for the mouse skin tissues, while 20% GLY with dry-ice freezing was effective for cattle skin tissues.
  Wu Tian-Wen , Li-Ting , Feng-Kai , Mao-Liu-Liu , Li Bi-Chun and Wu Xin-Sheng
  Researchers had scanned the complete CDS of rabbit KAP3.1 and KAP6.1 gene for the 1st time by PCR-SSCP and detected that their homology with mouse, chimpanzee, human, sheep and goat were >80%, suggesting that the two genes among different species kept high conservation in evolution. In this study, three genotypes and two alleles were found at KAP3.1-1 locus having one silent mutation (C91T) and there were two alleles and three genotypes in 5 un-translated region of KAP6.1 gene with the A 755 G mutation of the enhancer region. Herein, the distribution of these genotypes in all populations was consistent with the Hardy-Weinberg equilibrium and showed intermediate or low diversity. The result suggested a theoretical reference to the breeding of rabbit wool quality whether KAP3.1 and KAP6.1 gene could be a molecular marker or not.
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