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Articles by Leyla ACIK
Total Records ( 3 ) for Leyla ACIK
  Ilyas Bozlagan , Abdurrahman Ayvaz , Fatma Ozturk , Leyla Acik , Mikail Akbulut and Semih Yilmaz
  Sixty Bacillus spp. isolates were obtained from different agricultural fields in Kayseri province using selective media. Total DNA was isolated and analyzed by PCR using cry1 general primers. Of the 60 tested isolates, 17 carried the cry1 gene. B. thuringiensis subsp. kurstaki and all other isolates exhibited approximately 19-kb plasmid bands. In addition to the 270-bp band, the standard strain and isolate I47 yielded 180-bp PCR products. The isolates and standard strain were toxic to Ephestia kuehniella Zeller and Plodia interpunctella Hubner larvae. Insecticidal activity of isolates I36, I37, and I40 against Ephestia kuehniella was higher than that of the standard strain when 106 spore-crystals mL-1 was used. LC99 values for isolates I36, I37, and I40 were 6.46, 6.28, and 6.24 for E. kuehniella, respectively, and these isolates were also effective against Plodia interpunctella larvae. LC99 values for isolates I36 and I40 were 6.69 and 5.07 for P. interpunctella, respectively. The results clearly show that isolates I36, I37, and I40 were effective at lower doses, as compared to the others.
  Neslihan KAVALCIOGLU , Leyla ACIK and Munevver PINAR
  Random amplified polymorphic DNA (RAPD) is one of the easiest and most commonly used molecular techniques for genetic variability analysis. In the present study, the genetic diversity of analysis of Bellis perennis L. collected in 4 Turkish geographical locations was investigated by RAPD-PCR analysis. In addition to RAPD, pollen analysis of the 4 plant populations was also conducted. Out of 40 RAPD primers tested, 12 exhibited distinct banding patterns showing 45% to 79% polymorphism. Plants from these 4 localities were almost the same morphologically. Considerable differences were observed in the RAPD profile and pollen shape of plants from the Rize/Derekoy location. The results of this study clearly show that there are significant variations in the field collected populations of B. perennis from different geographic and climatic locations in Turkey. Environmental conditions may help us to determine the genomic structures of species.
  Emine ARSLAN , Ayten CELEBI , Leyla ACIK and Uckun Sait UCAN
  The objective of this work was to study the protein patterns, plasmid profiles, and antibiotic susceptibility of Staphylococcus intermedius and Staphylococcus aureus isolates originating from mastitic mammary glands of dairy cattle in different parts of Konya province. A total of 114 Staphylococcus species were isolated and identified by conventional bacteriological methods from bovine mastitis. Of the total isolates 77 were identified as S. aureus and 37 as S. intermedius. Intra- and inter-species diversities in the coagulase-positive staphylococci were investigated by analysis of whole-cell protein profiles using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Plasmid profiling also demonstrated that 75 S. aureus isolates and 36 S. intermedius isolates contained plasmid. In addition, 88.3% of S. aureus and 59.4% of S. intermedius isolates were resistant to penicillin. Sixty-six of the 77 S. aureus isolates were also resistant to amoxicillin+clavulanic acid (85.7%). The corresponding number for S. intermedius was 17 (45.9%). Only 1 S. aureus isolate was resistant to danofloxacin. One of each of the Staphylococcus isolates was resistant to methicillin. Results from the study showed that the susceptibility of S. intermedius isolates to antibiotics used widely in mastitis therapy is a matter of concern.
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