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Articles by Leila Jahangiri
Total Records ( 2 ) for Leila Jahangiri
  Leila Jahangiri , Seyed Adel Moallem , Tahereh Foroutan , Ahmad Hosseini and Fatemeh Nazemian
  Mesothelial progenitor cells have been reported to reside in either the monolayer of mesothelium, submesothelium or within the peritoneal cavity as free floating cells. A putative plasticity has been suggested for these cells as an epithelial to mesenchymal transition and transformation into myofibroblasts and smooth muscle have been suggested. In order to investigate the plasticity and nature of mesothelial cells, cell populations from peritoneal dialysis fluid of early stage non-peritonitis patients were first screened for dominant marker determination by RT-PCR and immunofluorescence. Then, cell colonies were isolated by culture and FACS using HBME-1 and CD34 markers. Efficacy of cell colony isolation by the mentioned methods was validated by flow cytometry. Later, specific media for the differentiation of the mesothelial colonies were defined. The culture of mesothelial cell colonies in knockout serum cultures containing specific growth factors showed a surprising but a relatively low yield of differentiation capacity along extra mesodermal lineage directed to neurons. This was evident by morphological characteristics of neurons and expression of neuronal specific cell markers consisting of the immature neuron markers Tubulin III and Nestin and also the structural neuronal marker Neurofilament 200 as revealed by Western blot. This study could completely violate the previously assumed plasticity of mesothelial progenitor cells and lead us to the definition of a new source of adult stem cells.
  Seyed Adel Moallem and Leila Jahangiri
  Mesothelial progenitor cells have been reported to reside in either the monolayer of mesothelium, submesothelium or within the peritoneal cavity as free floating cells. As a putative plasticity has been reported for the mesothelial progenitor cells and considering the potential implications of the establishment of a novel resource of stem /progenitor cells in gene and cell therapeutics and tissue engineering, we conducted an in vivo tracking of transplanted mesothelial cells. In order to induce immunodeficiency, the recipient mice were treated with 32 mg kg-1 of daily Cyclosporine. On days 14, 30 and 60 post transplantation, brain, heart, skeletal muscle and lung tissues were screened by a modified FISH method directed to the Y chromosome of donor cells. Fluorescence harboring cells were analyzed by flow cytometry and fluorescent microscopy. The data confirmed by PCR, demonstrated the existence morphology alteration of the donor cells in various organs of the recipient mice, notably in the skeletal muscle and lung and less in the heart and brain. Immunostaining of recovered cells from the nervous recipient tissues suggests differentiation of mesothelial cells in the new microenvironment.
 
 
 
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