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Articles by Lei Chen
Total Records ( 13 ) for Lei Chen
  Zhiwei Niu , Qiaohui Fan , Wenhua Wang , Junzheng Xu , Lei Chen and Wangsuo Wu
  Attapulgite was investigated to remove UO22+ from aqueous solutions because of its strong sorption capacity. Herein, the attapulgite sample was characterized by Fourier transform infrared spectra (FTIR), X-ray diffraction (XRD) and acid–base titration in detail. Sorption of UO22+ on attapulgite was strongly dependent on pH values and ionic strength. The presence of humic acid enhanced the sorption of UO22+ on attapulgite obviously because of the strong complexation of humic acid (HA) with UO22+ on attapulgite surface. Sorption of UO22+ on attapulgite was mainly dominated by ion-exchange or outer-sphere complexation at low pH values, and by inner-sphere complexation at high pH values. The results indicated that attapulgite was a suitable material for the preconcentration and solidification of UO22+ from large volume of solutions because of its negative surface charge and large surface areas.
  Lei Chen
  Resource scheduling is a extremely complex problem in grid computing. The performance of Grid strongly depends on the efficiency of resource scheduling. But, resource scheduling needs more optimal resource scheduling algorithm, otherwise it increases the cost and completion time and decreases the resource utilization efficiency. To improve performance of grid computing and resource utilization efficiency, it is urgent to design an optimal resource scheduling algorithm. This paper proposes Grid resource scheduling algorithm based on ant colony optimization. The proposed algorithm can achieve optimized resource allocation policy based on the users’ demand and improve the system’s performance. The new resource scheduling algorithm is implemented and its advantages are investigated in the Gridsim simulator. The simulation results show that the application of resource scheduling algorithm based on ant colony optimization can effectively reduce total task completion time, balance the load of system well and improve the efficiency of resource scheduling.
  Desheng Li , Lei Chen , Chengdong Wang , Hongbo Dai , Mengliang Liu , Ling Zhu , Xiaoyu Wang , Zhiwen Xu , Wanzhu Guo and Heng Min Cui
  The nasal bacterial flora of seven giant pandas was identified by 16S ribosomal RNA (rRNA) gene sequencing analysis. The most widely occurring species were Staphylococcus and Escherichia coli. Several species not known to be associated with giant pandas were present in the study including Pantoea agglomerans, Corynebacterium auriscanis, Kurthia gibsonii and Corynebacterium glutamicum.
  Feng-Chao Yan , Yong-Xi Dou and Lei Chen
  In this study, the Relative Synonymous Codon Usage (RSCU) values, Effective Number of Codon (ENC) values, nucleotide contents and dinucleotide were used to consider codon usage pattern of each structure protein gene and genome among the members of Morbillivirus genus. The results showed that the overall extent of codon usage bias in Morbilliviruses is low (mean ENC = 54.84>35). The good correlation between the (C+G) 12% and (G+C) 3% suggested that the mutational pressure rather than natural selection is the main factor that determines the codon usage bias and base component in Morbilliviruses. It is observed that synonymous codon usage pattern in Morbilliviruses can be divided into two groups. Under-represented CpG is a characteristic of all the members in Morbilliviruses. It may be a strategy to adapt to the host. These analyses not only provided an insight into the variation of codon usage pattern among the genomes of Morbilliviruses but also were helpful in understanding the processes of the evolution of Morbilliviruses.
  Lei Chen , Yongxi Dou and Xuepeng Cai
  Bibliometric methods were used to analyze publications from the Web of Science on Peste des Petits Ruminants (PPR) in the past 18 years. Results showed that although PPR is now a widespread and serious disease in many developing countries, the total number of articles on PPR is only 342. The research indicates that more basic studies should be done on the PPR and differentiating infected from vaccinated animals vaccines which would be important to prevent the disease. Moreover, collaborations among authors, institutions and countries as well as good financial support are the keys to control or even eradicate PPR.
  Zuo Zhicai , Hongbo Dai , Lei Chen , Yuancheng Zhou , Wanzhu Guo and Zhiwen Xu
  Haemophilus parasuis mainly affect piglets during suckling period and the infected pigs showed clinical symptoms at 5-6 weeks old after weaning. To develop a specific PCR for early detection of Haemophilus parasuis, early treatment would effectively prevent phenotypic expression of the disease. A pair of specific primers were designed to amplify a 414 bp specific DNA fragment based on H. parasuis Outer Membrane Protein (OMP) gene published in GenBank also the PCR conditions were optimized. The established PCR test could detect a minimum of 425 cfu mL-1 (0.425 cfu/PCR reaction) pure cultures of H. parasuis and only H. parasuis could amplify a 414 bp specific DNA fragment. No amplification was observed while tested with the closely affiliated species and the mainly contaminating flora. From 2 days post infection (dpi) to 5 dpi, all of the piglets challenged with H. parasuis showed a fever above 41°C and all blood samples from those feverish piglets were positive for H. parasuis. Through blood PCR detection, 37 positive samples were picked out in 84 sucking pigs which were natural infected with H. parasuis, the result was verified by dissecting five positive piglets and isolating bacteria from lung tissue. The developed PCR was successfully applied for early diagnosis of H. parasuis infection, early treatment will reduce the incidence and mortality of disease.
  Xiaolin Wang , Lei Chen , Junjie Yang , Fei Hua , Yunsheng Yu , Yanqiu Hu , Wenxue Ye , Haoyue Huang , Yihuan Chen , Hong Nin , Qingwei Ding and Zhenya Shen
  To evaluate the impact of Histidine-Tryptophan-Ketoglutarate solution (HTK solution, Koehler Chemie, Germany) for coronary perfusion in cardiopulmonary bypass on the risk of post-operative Acute Renal Failure (ARF) requiring Renal Replacement Therapy (RRT). The data of patients aged over 18 were retrospectively analyzed for the Cardiopulmonary Bypasses (CPB) performed in our Heart Center from April 2012 to October 2013. According to Simplified Renal Index (SRI) Scoring System, the patients were divided into two groups: the experiment group (using HTK solution and the control group (using cold blood cardioplegia solution). The actual occurrence rate of RRT for the postoperative ARF was analyzed for both groups. Furthermore, the pre and post-operative Glomerular Filtration Rate (GFR) were also compared between the two groups. According to SRI Scoring System, 512 patients were selected: 239 cases in the experiment group and 273 cases in the control group. The probability of RRT for post-operative ARF was 0.42% in the experiment group and was 4.03% in the control group (p<0.05). Comparing the experiment group and the control group with a score of ≥3 (48 cases in the experiment group and 44 cases in the control group), the occurrence rate in the control group (22.73%) was significant higher than that in the experiment group (2.08%). The pre and post-operative GFR levels in patients with a score of ≥3 were continuously monitored. No obvious statistical difference (p>0.05) was observed pre-operation (HTK: 72.6±32.93; cold blood: 72.73±29.31), on the 1st post-operative day (HTK: 62.38±26.76; cold blood: 58.89±23.22) and at the discharge day (HTK: 86.40±19.99; cold blood: 81.51±33.95). However, the GFR level of HTK solution group was significantly higher than that of cold blood group on the second (HTK: 73.13±26.01; cold blood: 61.10±23.40; p<0.05) and the 3rd post-operative days (HTK: 84.99±25.07; cold blood: 69.62±32.50; p<0.05). The SRI Scoring System was able to distinguish the low or high risk patients before cardiac surgery. For patients with an SRI score of ≥3 using the HTK solution for coronary perfusion could effectively reduce the risk of RRT for post-operative ARF and protect renal function.
  Yingzhi Wang , Lei Chen , Desheng Li , Ling Zhu , Wanzhu Guo and Zhiwen Xu
  A Gram-positive coccus isolated from a Giant Panda was identified by 16S ribosomal RNA (rRNA) gene sequencing analysis. Phylogenetic analysis showed that the organism was a member of the family Rothia. The 16S rRNA gene sequence of the organism showed 100% similarity with R. nasimurium strain CCUG 35957. Animal experiments were conducted to study the pathogenicity of R. nasimurium SCGP. This is the first reported isolation of R. nasimurium from the upper respiratory tracta of Giant Panda.
  Xiaobing Jiang , Liang Zhang , Siqing Chen , Ketian Wang , Zhuping Chen , Lei Chen , Jinyong Wang and Li Zhu
  Ultrasound measure is an important tool to evaluate carcass merit in live swine and the accuracy of ultrasonic measurement directly related to the breeding process. Here, researchers studied the effects of machine, posture and technician factors on the accuracy of ultrasonic measurement in live swine. The results indicated that the accuracy of A-mode ultrasonic machine measurement was lower than B-mode ultrasonic machine measurement and the accuracy of B-mode ultrasonic machine with equipped 3.5 MHz linear array probe performed the highest accuracy. Measured through the ultrasonic machine back fat thickness obtained from both the posture of bowing back (p<0.01) and standing posture with mouth contacting with the ground (p<0.05) were statistically significant different from the real carcass backfat thickness. Obtained from the posture of pigs were standing naturally both backfat thickness and logissimus muscle area were most approximated to the true value. During the operation of ultrasonic machine, the value of backfat thickness recognised by unexperienced technicians was statistically significantly different (p<0.01) from the true value. Thus in the process of measure both back fat thickness and logissimus muscle area through ultrasonic machine, the types of machine, the posture of pig standing and the skill level of technicians were validated to influence the obtaining of true value. In conclusion, the results indicated that the accuracy of ultrasonic measurement can be improved by suitable machines and probes, obtain picture while pig are standing naturally and skilled technicians.
  Lei Chen
  The panel data of the Chinese 31 provinces, autonomous regions and municipalities directly under the central government in 1995-2011 are used to build dynamic panel data model to investigate the influence of the Chinese local government's public investment on regional economic growth in this studay. And the research shows that since 1995 the Chinese local government’s public investment has promoted the regional economic growth but the effects of different public investment projects in different regions show big differences. Some improvement opinions are put forward in the end of the studay.
  Dan Hu , Lei Chen , Zhi-Quan Pan , Huan Liu , Xiang-Gao Meng and You Song
  A new trinuclear complex, lcub[Cu(L)]33-CO3)rcub(ClO4)4 (L = N-(2-thiophenoethyl)-N,N-bis (3-aminopropyl)amine), was synthesized and characterized by single-crystal X-ray analysis. The complex contained three identical mononuclear copper(II) units connected by the μ3-carbonate formed from atmospheric carbon dioxide. The electronic and magnetic properties were studied by cyclic voltammetry and the measurement of magnetic susceptibility, respectively. The μ3-bridging model revealed weak ferromagnetic coupling of Cu(II), with the J value of -11.28 cm-1 and the Zeeman splitting g value of 2.06, which were determined by means of magnetic measurements in the 2-300 K range.
  Lei Chen , Jin-Jiang Wang , Guo-Gang Zhang , Hong-Tao Song and Lu-Ping Qin
  A new cerebroside (1) was isolated from the aerial parts of Gynura divaricata DC., along with five known compounds: kaempferol (2), kaempferol 3-O-glucoside (3), kaempferol 3-O-rhamnosyl(1 → 6)glucoside (4), β-sitosteryl glucoside-6'-O-heptadecoicate (5) and 2-(1', 2', 3', 4'-tetrahydroxybutyl)-6-(2'', 3'', 4''-trihydroxybutyl)-pyrazine (6), which are isolated for the first time from this species. The structure of 1 was determined to be 1-O-β-D-glucopyranosyl-(2S, 3S, 4R, 10Z)-2-[(2'R)-2'-hydroxylignocenoyl-amino]-10-octadecene-1,3,4-triol on the basis of chemical and spectroscopic evidence.
  Shuo Chen , Jelica Gluhak-Heinrich , Marcos Martinez , Tong Li , Yimin Wu , Yimin Wu , Hui- Hsiu Chuang , Lei Chen , Juan Dong , Isabel Gay and Mary MacDougall
  Dentin sialophosphoprotein (DSPP), an important odontoblast differentiation marker, is necessary for tooth development and mineralization. Bone morphogenetic protein 2 (BMP2) plays a vital role in odontoblast function via diverse signal transduction systems. We hypothesize that BMP2 regulates DSPP gene transcription and thus odontoblast differentiation. Here we report that expression of BMP2 and DSPP is detected during mouse odontogenesis by in situ hybridization assay, and BMP2 up-regulates DSPP mRNA and protein expression as well as DSPP-luciferase promoter activity in mouse preodontoblasts. By sequentially deleting fragments of the mouse DSPP promoter, we show that a BMP2-response element is located between nucleotides –97 and –72. By using antibody and oligonucleotide competition assays in electrophoretic mobility shift analysis and chromatin immunoprecipitation experiments, we show that the heterotrimeric transcription factor Y (NF-Y) complex physically interacts with the inverted CCAAT box within the BMP2-response element. BMP2 induces NF-Y accumulation into the nucleus increasing its recruitment to the mouse DSPP promoter in vivo. Furthermore, forced overexpression of NF-Y enhances promoter activity and increases endogenous DSPP protein levels. In contrast, mutations in the NF-Y-binding motif reduce BMP2-induced DSPP transcription. Moreover, inhibiting BMP2 signaling by Noggin, a BMP2 antagonist, results in significant inhibition of DSPP gene expression in preodontoblasts. Taken together, these results indicate that BMP2 mediates DSPP gene expression and odontoblast differentiation via NF-Y signaling during tooth development.
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