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Articles by Latife BEYAZ
Total Records ( 2 ) for Latife BEYAZ
  Latife BEYAZ , Ayhan ATASEVER , Feyzullah BEYAZ and Anıl ICA
  The expression of the calcium-binding protein S100 and its subunits α and β in the intracellular protozoon Eimeria tenella was investigated. For this purpose, anti-S100 whole molecule polyclonal antibody and anti-S100 α and anti-S100 β monoclonal antibodies were used on paraffin sections collected from the cecum, ileum, colon, rectum, and bursa of Fabricius of chicks experimentally infected with E. tenella. One hundred seventy-five 2- to 3-day-old specific pathogen-free chicks were used. The chicks were divided into experiment and control groups, to include 94 and 81 chicks, respectively. All the chicks in the experimental group received 10,000 spored oocytes in 0.5 ml inoculum in a single dose. The duration of the experiment was limited to 18 days. All 175 chicks were sacrificed by cervical dislocation between hours 1 and 432 following inoculation. The streptavidin-biotin complex method was used to detect the immunoreactivity of the antibodies used. The results revealed a positive immune reaction for the S100 whole molecule and for α and β subunits in the schizonts, microgametes, macrogametes, and oocytes of the E. tenella in the cecum, ileum, colon, rectum, and bursa of Fabricius. These results show that both α and β subunits of the S100 protein existed in all the endogenous phases of E. tenella. These proteins are likely to mediate the parasitic development of E. tenella within host cells.
  Latife Beyaz , Ayhan Atasever , Fuat Aydin , K. Semih Gumussoy and Secil Abay
  This study aimed to experimentally induce Salmonella enterica subsp. enterica serovar Enteritidis (Salmonella Gallinarum) infection in turkey poults in order to detect S. Gallinarum using immunohistochemical, bacteriological, and histological procedures. The study included 90 white turkey poults that were divided into 3 groups (1-day-old, 3-week-old, and 2-month-old), each of which was orally inoculated with an inoculum of 0.3 mL of S. Gallinarum strain 9 broth culture that contained approximately 2 x 104, 1 x 106, and 1 x 109 cfu/mL, respectively. The duration of the experiment was 18 days. Clinical, pathological, and bacteriological findings were evaluated daily until 18 days post inoculation (pi). The most evident clinical symptoms in all 3 groups were diarrhea and somnolence, especially in 1-day-old group. S. Gallinarum strain 9 was isolated from organs and cloacal swabs up to 16 days pi. Inflammation and typical granulomatous nodules in the internal organs were observed in all 3 groups. Strong immunoreactivity was determined in the lungs, bursa of Fabricius, caecum, ileum, and cloaca of all infected poults up to 18 days pi. In conclusion, S. Gallinarum infection caused heterophilic granulomas, especially in the duodenum of turkey poults, and immunohistochemical analysis can be considered as an adjunct to bacteriological methods in the diagnosis of S. Gallinarum infection.
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