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Articles by Latifa Askarne
Total Records ( 2 ) for Latifa Askarne
  Idriss Talibi , Latifa Askarne , Hassan Boubaker , El Hassane Boudyach and Abdellah Ait Ben Aoumar
  The aim of this study was to find an alternative to the chemical fungicide currently used in the control of postharvest citrus sour rot. Here we screened thirty-two salt compounds, considered as common food additives, for their activity against Geotrichum candidum, causal agent of citrus sour rot. The lowest Minimum Inhibitory Concentrations (MICs) values were obtained by ammonium carbonate and EDTA at a concentration of 0.1% (w/v) and boric acid, sodium carbonate and sodium metabisulfite at 0.25% (w/v). Over all, the medium-pH in the range of 4.0 to 12.0 did not influence the mycelial growth of the pathogen. The ten best salt compounds were tested for their ability to reduce the arthrospores germination of the fungus. The effect of salts varied significantly (p<0.05) between tested compounds and depended on their concentrations. The arthrospore germination was completely inhibited by EDTA, boric acid, sodium metabisulfite, sodium carbonate, sodium sulfate and sodium thiosulfate, both at 100 and 75 mM. The most active salts in in vitro studies were tested in vivo against sour rot on citrus fruit. Incidence of sour rot was lowered to 25.93 and 38.89%, when mandarin fruit where treated by sodium salicylate, boric acid and EDTA, compared with 100% in the control. However, only the application of boric acid at 3% (w/v) reduced disease severity by more than 70%. These results suggest that sodium salicylate, boric acid and EDTA may be useful and effective compounds for control of citrus sour rot. Such healthy products therefore represent a sustainable alternative to the use of guazatine mainly in organic production.
  Fayza Tahiri Alaoui , Latifa Askarne , Hassan Boubaker , El Hassane Boudyach and Abdellah Ait Ben Aoumar
  Background and Objective: Tomato is the major fruit crop produced and exported in Morocco. This commodity is faced to many threats. The most important tomato diseases caused commercially significant losses, in Morocco and worldwide, is gray mold caused by Botrytis cinerea. This study was aimed to find out an alternative to synthetic fungicides used in the control of the polyphagous devastating fungus ‘Botrytis cinerea’ using common food additives. Materials and Methods: Thirty seven organic acids and salts considered as common food additives were tested in vitro against this pathogen using the agar dilution method. Compounds with the best antifungal activity, selected after one-way analysis of variance, were tested in vivo on artificially inoculated tomato fruit. Results: At 0.02 M, EDTA, copper sulfate and sodium metabisulfite completely inhibited the mycelial growth and sporulation of B. cinerea. The lowest Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) were recorded in sodium metabisulfite treatment. The conidia germination was inhibited by ammonium molybdate and sodium metabisulfite treatments at only 10 mM. The nine most active chemicals in the in vitro trials were tested in vivo on tomato fruit. The incidence and the severity of gray mold were significantly reduced by EDTA, potassium carbonate, sodium bicarbonate, sodium carbonate, sodium metabisulfite and sodium salicylate compared to 100% (incidence and the severity) in the control. Conclusion: The results of the current study suggest that these salts are potentially useful as postharvest GRAS compounds to control B. cinerea on tomato fruit.
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