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Articles by Lakshman Santra
Total Records ( 2 ) for Lakshman Santra
  Lakshman Santra , Saurabh Gupta , Ajay K. Singh , Amit R. Sahu , Ravi Kumar Gandham , Soumen Naskar , S.K. Maity , Jyotirmoy Ghosh and Sujoy K. Dhara
  The conventional method of Bone Marrow Stromal Cell (BMSC) isolation from live subjects are complex due to involvement of lot of expert personnel and pre and post operational medication and cares. Moreover, the concerning ethical issues also pose lots of restrictions for isolation of BMSC’s making stem cells research restricted to certain elite laboratories only. This study aims to compare between the regular aspiration (invasive) method and an alternative, straight forward and non-invasive method of BMSC harvest. The BMSCs were harvested by both invasive and non-invasive methods and cultured in MSC (Mesenchymal Stem Cell) medium. The cells were undergone visual assessment of growth dynamics as well as identification and characterization of MSC cells, based on microscopic examination. Both of these tested methods successfully yielded significant amount of BMSC that were found to be identical in morphology, growth dynamics and in vitro cultural properties. Unlike the invasive method that requires a live animal, the non-invasive method relies on post-slaughtered bone and therefore obviates the requirement of skill personnel and setup. Eventually, the used bone from already dead animal no longer becomes the issue of conflict with animal ethics and welfare. The ease in cell harvest and lack of ethical barrier would definitely make BMSC harvest convenient and therefore, anticipated to be well accepted in resource poor laboratories around the globe.
  Lakshman Santra , Saurabh Gupta , Ajay Kumar Singh , Manish Mahawar , Ravikumar Gandham and Sujoy K. Dhara
  The RNA interference is an established phenomenon of eukaryotic gene silencing. Recent advancement of genetic engineering allows employ this powerful tool for silencing multiple genes in several domestic animals and thus adds an additional dimension to animal welfare and livestock product improvement. This study reviews the current state of and prospects for the development of transgenic livestock using the mighty tool RNAi. The mini review accesses and summarizes information available in listed references. It starts with brief introduction to basis of RNA interference followed by outlining the current application of RNAi mediated transgenesis in livestock sector and identifies potential ways of developing and improving genetically modified livestock produces. This mini review explains importance of RNAi as a tool for improving animal produce. Here we present highlights of different recent studies that describe gene silencing by RNAi as an intervention tool for improvement of quality of various animal produces, acceleration of postnatal body growth, protection of livestock animals from certain fatal diseases etc. Diverse approaches are described to identify potential ways of developing genetically modified livestock. Further, this study emphasizes on recent advances in the field of gene targeting mentioning briefly on diverse molecular scissors and their potential involvement in executing conditional knock out or shRNA mediated knockdown strategies. Overall this mini review provides an update of the current status of harnessing RNAi for intended livestock produce and also points to the trend of future transgenic animal husbandry towards making the concept of ‘an animal for all purpose’ to come into reality.
 
 
 
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