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Articles by Laila Ahmed Rashed
Total Records ( 2 ) for Laila Ahmed Rashed
  Reham Abdel-aal Awad Morsy , Salwa Mahmoud Sarhan , Laila Ahmed Rashed , Mohamed Gomaa Attia-Zouair , Mohamed Mahmoud Ahmed , Mona Mahmoud El-Batran and Nadia Lashin Soliman
  Background and Objective: Many lines of evidence suggested that the oxidative stress is important in pathogenesis of diseases. Nicotine inhibited the proliferation and osteoblastic differentiation of mesenchymal stem cells (MSCs) and decrease alkaline phosphatase (ALP) activity. The biological antioxidants, such as vitamin E act in synergy to form an integrated network of antioxidant defense, which is considered to be the first line of defense against any increase in the production of reactive oxygen species (ROS). The objective of the study was to investigate the effect of vitamin E on the proliferation as well as differentiation potentiality of rat’s dental follicle stem cells (DFSCs) treated with nicotine. Materials and Methods: Rat DFSCs were isolated, cultured and divided into four groups. The 1st group was the control, the 2nd group was cultured with 5 mM of nicotine, the 3rd group was cultured with 2mM of vitamin E (1h) prior to nicotine treatment and the 4th group was cultured with 2 mM of vitamin E (1 h) after nicotine treatment. Methyl thiazol tetrazolium (MTT) assay was used to assess the cell viability among the groups used. Wright staining solution was used to assess the effects of nicotine on cell morphology. The flow cytometric analysis was used to identify the stemness of isolated rat’s DFSCs by CD34 and CD29. Alizarin red-sulfate (AR-S) staining and quantitative real time-polymerase chain reaction (qRT-PCR) utilizing alkaline phosphatase (ALP) expression and activity were used to determine the osteoblastic differentiation. Results: The results of this study revealed that increase the proliferation rate and the ALP expression and activity in group I followed by group III and IV then group II. The vacuolization and loss of cell membrane of DFSCs appeared to be more extensive in group II compared to group III and group IV. Conclusion: These findings indicated that vitamin E could relief the state of oxidative process induced by nicotine. Vitamin E also can increase the capability of DFSCs to proliferate and differentiate into osteogenic lineage detected by AR-S stain and increase the ALP level.
  Rania Mohamed Abdelmohsen Elmahy , Gihan Samir Mohamed and Laila Ahmed Rashed
  Background and Objective: The purpose of the study was to investigate the influence of laser applied on acupuncture points in endothelial dysfunction on hypertensive obese patients. Materials and Methods: Sixty patients from both genders aged between 40-50 years old were assigned into two groups equal in number. Group A: Received a program of aerobic exercises and laser on acupuncture points 16 J per point and group B: Received a program of aerobic exercises and sham laser (placebo) on acupuncture points. The program was 2 times per week for 24 weeks. Urinary Nitric Oxide (NO) and blood superoxide dismutase (SOD) were measured at the beginning and at the end of the study. Results: The results of study group revealed that there was a significant increase in nitric oxide (81.82%) and superoxide dismutase (78.72%) levels, which means reduction in oxidative stress and improvement in endothelial function. Conclusion: It could be concluded that for hypertensive patients using laser puncture two times per week improved their nitric oxide and superoxide dismutase levels which is important antioxidants in the body. Laser applied on acupuncture points improve general health.
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