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Articles by La Ode Nafiu
Total Records ( 2 ) for La Ode Nafiu
  Muhammad Amrullah Pagala , Takdir Saili , La Ode Nafiu , Nasir Sandiah , La Ode Baa , Achmad Selamet Aku , Deki Zulkarnaen and Widhi Kurniawan
  Background: The Mx gene plays a crucial role in the antiviral responses of chicken. The Mx gene codes for Mx protein, which possesses antiviral traits. The non-synonymous G/A single nucleotide polymorphism (SNP) at position 2032 of chicken Mx cDNA results in a change at amino acid 631 of the Mx protein. This mutation affects the antiviral activity of the Mx molecule. Objective: The aim of the study was to evaluate the genetic polymorphism of the Mx gene in the native chickens of South East Sulawesi, Indonesia, using PCR-RFLP. Materials and Methods: The Mx gene was genotyped in 25 Tolaki chickens from each of Konawe Regency and South Konawe Regency and in 21 Kampong chickens from Kendari City. Tolaki chicken is traditionally used as a medium for medicinal treatments in the Tolaki culture. PCR was used to amplify genomic DNA for the Mx gene (299 bp). The amplifier was cut using the Hpy81 enzyme. Results: The genotyping of the Mx gene of native chicken produced three genotypes, AA, AG and GG and two alleles, A (299 bp) and G (200 bp and 99 bp); the frequency of A was higher than that of G. The value of x2 showed that Mx|Hpy81 was in Hardy-Weinberg equilibrium. The values of He and PIC were 0.47-0.49 and 0.36-0.37, respectively. Conclusion: These results indicated that Mx|Hpy81 gene was polymorphic in all strains of chicken that were genotyped. The Mx|Hpy81 gene demonstrated a high potential for use as a genetic marker for resistance to Avian influenza and Newcastle disease infection in Indonesian native chickens.
  La Ode Nafiu , Takdir Saili , Ali Bain , Muhidin , Muhammad Rusdin and Rusli Badaruddin
  Background and Objectives: The main constraint on the development of buffalo in Southeast Sulawesi is the poor quality of the feed in the pasture, so growth tends to be slow and there is high mortality. The study aimed to investigate the response of selected heifer buffalo to feed improvement. Materials and Methods: The research was performed using a Completely Randomized Block Design (CRBD). There were 4 treatments based on the type of feed: T1 (natural grass), T2 (natural grass+concentrate (1 kg sago hampas+1.5 kg rice bran), T3 (elephant grass) and T4 (elephant grass+concentrate). Results: Treatments T2 and T4 produced average daily gains (0.502 and 0.574 kg head1 day1) respectively, significantly higher (p<0.05) than that with feeding on T1 or T3, which produced only 0.338 and 0.427 kg head1 day1, respectively. The addition of concentrate also decreased the value of feed conversion, i.e., those for T4 and T2 were only 8.637 and 10.160 kg head1 day1, respectively, significantly lower (p<0.05) than the T1 and T3 values of 11.463 and 14.928 kg head1 day1, respectively. Conclusion: The selected heifer buffalo showed a positive response to the feed improvement in the form of high daily gain and low feed conversion.
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