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Articles by L.X. Wang
Total Records ( 3 ) for L.X. Wang
  L.W. Zhai , L.X. Wang , W.L. Zhou and C.D. Wang
  This study investigated the polymorphism of MYPN gene, which codes the Myopalladin (Myop) protein, being chosen as a candidate gene for meat quality. Three SNPs were detected in the 3 UTR of MYPN. Association analysis of the MYPN genotype with meat quality of longissmus muscles was conducted in several western meat producing breeds, including Yorkshires (Y), Landraces (L) and LxY pigs (LY). This study showed that some of the meat quality traits exhibited significant difference in different breeds and different genotypes. The meat pH value, color (Opto-star value) and tenderness (shear force) of Yorkshires were significantly higher than those of Landrace and LxY pigs; on the other hand, LxY pigs had significantly higher conductivity and intramuscular fat content and water-hold capacity was significantly higher for Landrace than Yorkshires and LxY pigs. All the three detected SNPs were shown to be associated with meat color and tenderness, Whereas none showed association with conductivity. The pH value, tenderness and intramuscular fat content were associated to two SNPs, respectively, water-hold capacity was associated to only one SNP. This study suggests that MYPN gene can be investigated as a candidate gene of meat quality in the farther reseach.
  X.B. Zheng , X.M. Cen , L.X. Wang , G.Y. Xin , X.H. Fu , P. Liu , G.H. Li , M. Zhang , S.S. Lu and K.H. Lu
  Nanog is one of important transcription factors to maintain characteristics of pluripotent stem cells. The present study was to clone Nanog of Capra hircus to express His-Nanog protein in E. coli BL 21 cells and further to purify it. The total RNA was extracted from primordial genital ridge tissues of a fetal lam and by means of RT-PCR, Nanog gene was amplified which was subcloned to pET32a to construct its prokaryotic expression vector. Confirmed by restrictive endonuclease digestion and DNA sequencing, the recombinant plasmid was transformed into E. coli BL21(DE3) and His-Nanog fusion protein was expressed by the induction of IPTG and identified with SDS-PAGE analysis. Under denaturing condition, the His-Nanog protein was purified by using Ni-NTA resin and verified by Western blotting assay. The results showed that The Open Reading Frame (ORF) of Nanog gene in Capra hircus is composed of 903 nucleutide acids, coding 320 amino acids; SDS-PAGE assay showed that His-Nanog fusion protein was efficiently expressed in form of inclusion bodies in E. coli BL21 (DE3) inclusion bodies were solubilized in 6 mol L-1 GuHCl, His-Nanog fusion protein with higher purity was purified by using Ni-NTA resin; Western blotting assay showed that the purified His-Nanog could bind to anti-His tag antibody specifically indicating the expected immunogenicity. This recombinant protein could be used directly to prepare polyclonal or monoclonal anti-Nanog antibody which will lead to study Nanog’s function or characteristics of pluripoten stem cells (such as iPS cells) in Capra hircus.
  C.X. Chen , S.C. Li , S.Q. Wang , H.N. Liu , Q.M. Deng , A.P. Zheng , J. Zhu , L.X. Wang and P. Li
  The concept of a “core parent” was proposed by breeders to describe elite lines with both good field performance and high potential for breeding superior new lines. Guichao 2 is one of the most important rice core parents in China. In the present study, Simple Sequence Repeat marker (SSR) based genome-wide screening was performed for Guichao 2, its parents and derivatives to study the genetic diversity and structure of them. A total of 348 polymorphic markers and 833 polymorphic alleles were detected. The Polymorphic Information Content (PIC) value ranged from 0.15 to 0.95 and averaged 0.39. Genetic similarity among the ten varieties varied from 0.751 to 0.926 with an average of 0.842, indicating that the genetic diversity is not abundant. At the whole genome level, Chaoyangzao 18 (30.4%) contributed many more genetic components to Guichao 2 than Guiyangai 49 (16.3%) did. The amount of genetic material transmitted by Guichao 2 to its derivatives varied from 49.7% (Qingliuai) to 59.5% (Fengqingai), with an average of 54.7%. In addition, 78 genomic regions of Guichao 2 were identified as stably inherited by these derivatives, with contribution ratios ranging from 14.29 to 85.71%, with an average of 47.25%. Ten of these regions with the same contribution ratio of 85.1% (RM3412-RM140, RM1339-RM1068, RM6997-RM6172, RM3524-RM3042, RM1388-RM1136, RM1353-RM1243, RM5508-RM3753, RM3395-RM3662, RM3662-RM44 and RM6643-RM2915) were found to be significantly important in the derivative cultivars.
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