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Articles by L.S. Bilbis
Total Records ( 6 ) for L.S. Bilbis
  S.A. Habibullah , L.S. Bilbis , M.J. Ladan , O.P. Ajagbonna and Y. Saidu
  Reducing cholesterol content of table egg may be important in curbing the risks of atherosclerosis. In this study, forty, (32 week old) Shika Brown laying hens were randomly distributed into five groups to investigate the effect of aqueous extract of Hibiscus sabdariffa calyces on serum lipid profile and egg yolk cholesterol. Groups 2 - 5 received 1, 2, 4 or 8 g L-1 solution of the extract. Group 1 (control) received tap water. All the animals were fed ad libitum with layers mash for twelve weeks. Blood samples and eggs were collected after every three weeks and sera and egg yolk were analyzed for total cholesterol, triglycerides (TAG), high-density lipoprotein cholesterol (HDL-C) and egg yolk cholesterol respectively. Hibiscus sabdariffa extract caused a significant (p<0.05) non dose dependent increase in serum total cholesterol and egg yolk cholesterol but significant (p<0.05) dose-dependent decrease in serum TAG and HDL-C content. No strong correlation (0.2273) was found between serum and yolk total cholesterol. These results suggest that aqueous extract of Hibiscus sabdariffa may possess hypotriglyceridemic effect in Shika Brown laying hens but caused increase in serum and egg yolk cholesterol.
  S.W. Hassan , M. Lawal , B.Y. Muhammad , R.A. Umar , L.S. Bilbis and Y. Saidu
  The anthraquinone glycosides composition and toxicity studies of isolated anthraquinone glycosides and aqueous ethanol stem bark extracts of Ficus sycomorus on rat liver and kidney functions were conducted. The yield of anthraquinone glycosides in 40 g powdered stem bark extract was 16.0% (w/v). Liver and renal indices were significantly (p<0.05) changed at higher doses of 617.86, 988.57 (anthraqiunone glycosides), 767.80 and 1228.60 mg kg-1 body weight (aqueous ethanol extract). Dose dependant decrease in weight (p<0.05) was observed in the rats administered higher doses of the aqueous ethanol and anthraquinone glycosides extracts. The results suggest that extracts of Ficus sycomorus cause adverse effects to liver and kidney at higher doses.
  H.L. Muhammad , R.A. Shehu , L.S. Bilbis and S.M. Dangoggo
  Pollution by heavy metals deserves a special attention because of their high toxicities and persistence in aquatic environment, especially with respect to such ecosystems that receive chronic inputs of these metals. Physicochemical properties such as pH, Dissolved Oxygen (DO) and Biochemical Oxygen Demand (BOD) that can influence the availability of the metals in aquatic environment were investigated. Water, sediment and floodplain samples were digested by standard laboratory methods. Metal analyses on water, sediment and flood plain samples were carried out by Atomic Absorption Spectrophotometry (AAS). On the whole, the pH of water, sediment and floodplain samples were in the range of neutrality to a little alkalinity (6.52-7.70). The Dissolved Oxygen (DO) for the two seasons were significantly (p>0.05) low in all the study sites. Biochemical Oxygen Demand (BOD) for Rivers Bunsuru and Gagare were also significantly (p>0.05) low when compared with WHO standard of 7.0 mg L-1. Rima River and Goronyo Dam had BOD (p<0.05) on the high side. Except for Rima River, all other rivers had Cu concentrations significantly (p<0.05) high. Lead (Pb) concentrations during the rainy season sampling from River Rima and Goronyo Dam were present above the WHO acceptable limit of 2.0 μg g-1. Other mineral elements and Cd were however within the WHO limits. Significant levels of Pb in water, sediment and floodplain samples mean possible Pb toxicity to aquatic organisms and by implication man through food chain. Presence of Cd even at WHO acceptable limit is at the potential risk of bioaccumulation.
  S.W. Hassan , M. Lawal , B.Y. Muhammad , R.A. Umar , L.S. Bilbis , A.A. Ebbo and Y.U. Dabai
  The column chromatographic fractions of chloroform (CHL1, CHL2 and CHL3) root extracts of Boscia angustifolia were screened for antibacterial activity and phytochemical properties. CHL1 fraction was significantly active (p<0.05) at 5 to 60 mg L-1 on Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Streptococcus pneumoniae with Minimum Inhibitory Concentration (MIC) of 0.63 to 1.25 mg L-1. The active fraction (CHL1) revealed the presence of only alkaloids with Retention factor (Rf) value of 0.36. The active antibacterial agent in the most potent fraction (CHL1) was isolated and identified by thin layer chromatography (TLC) and phytochemistry. The CHL2 and CHL3 fractions did not show inhibitory activity at 5 to 60 mg L-1. The antibacterial activity of root extract of Boscia angustifolia is due to a chloroform-extractable compound. The results support the ethnomedicinal use of root of Boscia angustifolia for the treatment of bacterial diseases.
  S.W. Hassan , M. Lawal , B.Y. Muhammad , R.A. Umar , L.S. Bilbis , U.Z. Faruk and A.A. Ebbo
  The in vitro antifungal activity and phytochemical properties of column chromatography fractions of Hexane (HX), Petroleum Ether (PE) and Chloroform (CHL) of stem bark of Ficus sycomorus were studied. The bioactive agent in the most potent fraction with antifungal properties was isolated and identified by preparative Thin Layer Chromatography (TLC) and phytochemical analysis. The hexane fractions (HX2 and HX3) were significantly active (p<0.05) at 0.31 to 5.63 mg mL-1 on Microsporum gypseum, Aspergillus niger, Aspergillus flavus and Candida albicans. The fractions revealed the presence of Steroids (STR), Condensed Tannins (CDT), Cardiac Glycosides (CG) and Saponins (SAP). The HX4 fraction was the most potent, exhibiting complete inhibitory activity against all the fungal isolates tested. The fraction contained Anthraquinone Glycosides (ATG) with Retention factor (Rf) value of 0.87. All the PE fractions (PE1, PE2 and PE3) did not exhibit significant activity on the fungal isolates at 0.31 to 3.75 mg mL-1. The CHL fractions (CHL1 and CHL2) showed very high inhibitory activity on only Trichophyton mentagrophytes and Trichophyton rubrum. The phytochemical analysis of these fractions revealed the presence of CG, SAP, CDT and STR. The results of the present study add credence to the ethnomedicinal uses of Ficus sycomorus for the treatment of fungal diseases.
  I.U. Karaye , A.A. Aliero , S. Muhammad and L.S. Bilbis
  Amino acid composition and volatile organic compounds (VOCS) of five Nigerian cucurbits namely: Luffa aegyptiaca (Mill.), Citrullus lanatus (Thunb. Matsum), Cucurbita maxima (Duchesne, ex Lam), Cucumis metuliferus (E.Mey. ex Naudin) and Momordica balsamina (L.) were investigated using Amino acid Analyzer and Gas Chromatography-Mass Spectrometry (GC-MS). The proportion of essential amino acids ranged from 22.75- 30.23 g/100 g protein with the highest content in M. balsamina with 30.23 g/100 g protein and the least in C. lanatus with 22.75 g/100 g protein. High content of leucine was found in C. maxima and M. balsamina with 7.04 and 6.11 g /100 g protein respectively. The GC-MS analysis revealed the presence of thirty-one compounds, the most abundant classes of organic compounds in L. aegyptiaca comprised of esters with 65.17% and Fatty Acids (FA) had 32.62% while hydrocarbons amounted to 29%. In C. lanatus, seven compounds were identified dominated by FA and hydrocarbons with 91.5 and 7.25% respectively. In C. maxima, ten compounds were identified dominated by FA (52.24%) and hydrocarbons (43.46%). In C. metuliferus, FA had 30.74% while aromatic compounds and alcohol had 9.18 and 0.68%, respectively. Among the five seeds studied, the most abundant compounds identified were linoleic acid methyl ester in L. aegyptiaca with 63.72%. Cis-cis linoleic acid is most abundant in M. balsamina (62.03%) totaling the fatty acid portion to 86.41%. These results suggest the potential of the seeds as a source of amino acids and fatty acids that could be useful in food and feed fortification strategies. The unique VOCS could be useful as biomarkers for delimitation of the studied species.
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