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Articles by L.R. Daniel
Total Records ( 2 ) for L.R. Daniel
  A.S. Fairchild , J.L. Grimes , J.K. Porter , W.J. Croom Jr. , L.R. Daniel and W.M. Hagler Jr.
  Turkey poults were randomly placed in batteries and fed one of four dietary treatments: control (C); control plus 4ppm diacetoxyscirpenol (DAS); control plus 300 ppm (FA); and control plus 4ppm DAS and 300ppm FA (FD). There were 10 poults per pen with 6 replicate pens per treatment. Individual BW, BW gains (BWG) and feed consumption by pen was determined at d6, d12, and d18. Period and cumulative feed to gain was calculated. Mouth lesions were scored for treatments at d18. On d18 poults were euthanized for determination of organ weights and jejunal histomorphometrics. FA had no effect on BW or BWG at any period compared to C. Poults fed FD had reduced BW and BWG compared to C, while poults fed DAS had lower BW than all treatments at every period. Poults fed FA or C had better feed to gain (P<0.05) than poults fed DAS or FD at d6. There were no differences among the treatments at d12 or d18. Poults fed FA had significantly lower relative intestine wt than poults fed other diets, and significantly higher relative bursa wt at d18 when compared to poults fed DAS or FD. DAS, FA and FD altered intestinal architecture. Poults fed DAS or FD had higher mouth lesion scores than poults fed FA or C, but mouth lesion scores in DAS and FD poults were not different from each other. Dietary DAS resulted in decreased poult performance, while dietary FA had little or no effect. Fusaric acid fed in combination with DAS resulted in some protective effect towards DAS.
  W.J. Croom , Jr. , J. Decubellis , B.A. Coles , L.R. Daniel and V.L. Christensen
  Previous studies in this laboratory have demonstrated that peptide YY (PYY) administration to turkey poults at d25 of incubation enhances intestinal Na-dependent active glucose uptake. This study was designed to further characterize the ontogeny of glucose transport in embryonic and hatchling poults and to investigate the effects of PYY on this process during development. In Trial 1, 20 turkey eggs were randomly selected at days 20, 23, and 26 of incubation, as well as the day of hatch. Hatchlings were cervically dislocated and the body weight, jejunal length and jejunal weight were recorded. Jejunal glucose uptake was estimated by measuring 3H-3-O-methyl-D-glucose accumulation in 2 mm jejunal rings in vitro. Jejunal O2 consumption was measured in vitro on jejunal rings using an O2 probe. In Trial 2, 40 turkey eggs were randomly selected at days 20, 23 and 25 of incubation and injected, via the air sac, with either 0.9% saline or 0.9 % saline plus 400 μg PYY/kg egg weight. Embryos from each treatment were harvested on days 23, 26 and day of hatch. Measurements and analyses on jejunal tissue were conducted as in Trial 1. In Trial 2, embryonic weight and jejunal weight adjusted for body weight increased (p< 0.05) with stage of incubation, while adjusted jejunal length decreased (p< 0.01). Active and total glucose uptake and jejunal O2 consumption increased with age (p< 0.05). The energetic efficiency of glucose uptake increased (p< 0.05) between d26 and hatch. In Trial 2, PYY failed to significantly affect body or jejunal weight, glucose absorption, and O2 consumption at any stage of development. PYY did however, decrease the efficiency of glucose uptake at d26 and at hatch (p< 0.05). In contrast to earlier investigations using higher dosages of PYY, this study demonstrated that in ovo PYY administration at 400 μg/kg egg weight has little effect of jejunal function in turkeys.
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