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Articles by L.P. Yadava
Total Records ( 5 ) for L.P. Yadava
  L.P. Yadava
  Various doses of paclobutrazol (12.5, 25, 50 and 100 ppm) and ethephon (100, 200, 400 and 800 ppm) on flowering, fruit characters and quality of cape gooseberry (Physalis peruviana L.) were observed. Results indicated that ethephon 800 ppm delayed flowering but prolonged duration of fruit set and early ripening by 30.25, 2.5 and 10.13 days, respectively over control. 400 ppm of ethephon was more effective than 800 ppm in respect of fruit weight and quality constituents. In the same trend by 17.5, 2.37 and 5.85 days delay in flowering, prolonged duration of fruit set and early ripening, respectively were recorded with paclobutrazol 100 ppm. However, ethephon 400 ppm and paclobutrazol 50 ppm significantly influenced the fruit set, growth rate, cumulative growth, volume, density and TSS/acidity ratio of the fruit as compared to control and other concentrations of the retardants.
  A. Gupta , V.K. Gupta , D.R. Modi and L.P. Yadava
  The cultural and nutrient requirements of Aspergillus niger for production of α-amylase in production media containing different pH, temperature, incubation period, metal ion concentrations, surfactants, carbon sources and nitrogen sources were quantified in present study. The optimum pH, temperature and incubation period for enzyme production were 5.0, 30°C and 5th day, respectively. Of the carbon sources, starch at 0.5% was recorded to be the best carbon source for enzyme production. Peptone at 0.03% was ideal nitrogen source. However, surfactants Tween-80, Triton X-100 and Sodium dodecyl sulphate at 0.02, 0.002 and 0.0002% concentration were most effective for enhancement of α-amylase production. The main objectives of the present study were to use a suitable fungal strain for production of extra cellular alpha-amylase, to determine the time course for the production of alpha-amylase and to study the effects of external substances that may enhance the production of extra cellular alpha-amylase, including metal ions and surfactants.
  A. Pandey , M. Kamle , L.P. Yadava , M. Muthukumar , P. Kumar , V. Gupta , M. Ashfaque and B.K. Pandey
  In the context of the GM food regulations crop improvement via transgenic technology is a new stage of introducing novel food which supercedes over the conventional breeding. It was analyzed that worlds hunger, malnutrition problems, environmental pollution and phytoremediation in agriculture are the challenges for scientist as well as governments those can be combated by application of genetic engineering in crops. Genetically modified microbes/plant/animals or GM microbes/plant/animals results from modification in the genetic make-up of microorganisms, plants and animals using recombinant DNA technology to improve the nutritional requirement, disease resistant traits, increased production and medicinal properties. In many instances, these modification processes represent faster, more efficient mechanisms for achieving changes than traditional breeding. However, a wide variety of modifications are possible through genetic manipulation and the potential for the introduction of toxic compounds, unexpected secondary effects and changes in nutritional and toxicological characteristics may give rise to safety concerns about GM crops. Thus, generation of GM food explores new vistas for future food requirement but the assessment of policy regarding environmental risks is also to be concerned.
  A. Pandey , M. Kamle , L.P. Yadava , M. Muthukumar , P. Kumar , V. Gupta , M. Ashfaque and B.K. Pandey
  A. Pandey , B.K. Pandey , M. Muthukumar , L.P. Yadava and U.K. Chauhan
  Anthracnose, caused by Colletotrichum gloeosporioides, is a serious postharvest disease of mango. The histopathological studies on anatomy of naturally infected by Colletotrichum gloeosporioides and artificially inoculated leaves and healthy leaves were performed to understand the infection process of anthracnose at various intervals after inoculation. Germination and penetration processes of the pathogen within the whole leaf were observed. The first evidence of penetration into the whole leaf was observed 48 h after invasion. It also revealed that mycelia were prominent after 120 h after invasion by the fungus (C. gloeosporioides). Subcuticular infection by hyphae was present in transverse leaf sections (T.S.) of the diseased sample after 72 h. Also, both inter and intra-cellular hyphal invasion were observed after 72 h. Mesophyll cells were highly affected by fungal invasion and rapidly collapsed. Swelling of epidermal cell walls was also observed. After 96 h almost all the cells became necrotized (Nc). Necrotized mycelial mats (M) of C. gloeosporioides was observed after 120 h and all the invaded cells became necrotized (Nc) forming a spot which eventually the cells ruptured leaving a shot hole symptom. All these observations pertained to the cells of mesophyll tissue indicating that these are the regions of fungal invasion and host tissue damage resulting in the disease symptoms. Naturally infected and artificially inoculated (in vitro) presented no significant differences suggesting that the pathogen invasion and symptom development process is similar in both the conditions.
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