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Articles by L.N. Sankhala
Total Records ( 7 ) for L.N. Sankhala
  B.S. Saini , N. Kataria and L.N. Sankhala
  In the present investigation, the Extracellular Fluid (ECF) volume and serum electrolytes were determined in healthy adult female Marwari sheep during control, thirst, drinking and recovery periods. The control mean value of ECF volume was 9.187±0.332. Advancement in thirst period resulted in gradual deficit in ECF volume and on days 5 of thirst period 35.278% change was observed in ECF volume. Immediately after rehydration, replenishment was only 13.824%. Even on day 10 of recovery period, replenishment was not complete in ECF volume. The mean values of various serum electrolytes viz., calcium, phosphorus, magnesium, chloride and sodium increased significantly (p≤0.05) as the days progressed except potassium which decreased significantly (p≤0.05). On day 5 of thirst period, the values of calcium, phosphorus, magnesium, chloride and sodium were 37.892, 45.654, 61.687, 34.454, 21.805 and 24.428% higher, respectively than the corresponding mean value during control period. Drinking resulted in lowering of all the electrolytes than their respective day 5 thirst period values except potassium which showed an increase at hour 1/2 of drinking period. At 72 h of drinking period all the serum electrolytes showed non-significant (p>0.05) differences from respective control mean values. In present study no clinical signs of oedema, staggering or other illness was observed after thirst period and drinking periods. It was concluded that a thirst period of 5 days caused a state of dehydration resulting in water loss from ECF compartment. Owing to slow replenishment in the ECF volume, close monitoring of the dehydrated animals is essential to replenish fluid volume along with fluid therapy for a longer period.
  R. Maan , N. Kataria , P.K. Pilania , A. Sharma , S. Arora , A. Joshi , L.N. Sankhala , S.K. Sharma , N. Mohammad , P. Nathawat and A.K. Kataria
  Serum Superoxide Dismutase (SOD) profiling was carried out in the Marwari breed of sheep from arid tracts in India. Animals were categorized according to sex and age. Blood samples were collected during moderate, extreme hot and cold ambiences to harvest sera. Mean value of serum SOD during moderate ambience was 171.76±2.72 kU L-1. Moderate mean value was considered as control. The mean value of serum SOD was significantly (p≤0.05) higher during hot and cold ambiences as compared to control. Mean value was 2.57 times higher in hot ambience and 1.54 times higher in cold ambience as compared to control. The sex and age effects were significant (p≤0.05) in all ambiences. The mean values were significantly (p≤0.05) higher in male animals than female animals. In each ambience the age effect showed a significant (p≤0.05) increase in the mean values being highest in the animals of 2.5-4.5 years of age. Increased SOD activities during extreme ambiences indicated the development of oxidative stress. Present study generated data of SOD which can be used as reference values for future studies and for diagnostic purposes. The pattern of variation of SOD activity indicated modulation of adaptive mechanisms to protect the body from changes in ambient temperatures and on this basis use of antioxidants in the animals during changing environments is recommended.
  N. Kataria , Abhimanu , S. Arora , A. Sharma , R. Maan , S. Soren , A.K. Kataria , N. Mohammad , P.K. Pilania , L.N. Sankhala , M.K. Thori , J.S. Gaur and A. Meena
  A study was carried out in male and female calves of Rathi breed belonging to arid tracts from India to find out status of free radical scavenging enzymes during hot ambience. Blood samples were collected during moderate and hot ambiences to harvest sera. Maximum ambient temperature ranged from 44.5-46.5°C during hot ambience. All calves were managed in similar conditions and were apparently healthy. Free radical scavenging enzymes included Superoxide Dismutase (SOD), Glutathione Reductase (GR) and Catalase (CAT). The moderate (control) mean values of serum SOD, GR and CAT were 173.00±1.43, 11.00±0.09 and 74.80±1.00 kU L-1, respectively irrespective of gender and age. Similar pattern of significant change (p≤0.05) was observed by all the three enzymes in heat stressed calves being higher during hot ambience. The mean values were 2.67, 2 and 1.41 times higher, respectively in heat stressed calves during hot ambience. The sex and age effects were significant (p≤0.05) in moderate and hot ambiences for all the three enzymes. The mean values were significantly (p≤0.05) higher in male animals than female animals. In each ambience the age effect showed a significant (p≤0.05) increase in the mean values being highest in the calves of 6 months to 1 year of age. Results clearly indicated that status of free radical enzymes changed in heat stressed calves probably to scavenge higher number of free radicals formed during hot ambience. All the three enzymes used are important biomarkers of oxidative stress. Present study provided data which can be used as reference values to assess the extent of oxidative stress. This will help in timely protection of calves from ensuing health disorders.
  A. Sharma , N. Kataria , N. Arya , M. Mathur , R. Maan , A. Joshi , Abhimanu , L.N. Sankhala , T. Bhati and A.K. Kataria
  A study was conducted to assess modulations in gluconeogenesis in Marwari goat of both sexes ageing 6 months to 4.5 years during moderate, hot and cold environmental temperature periods. Liver samples were collected to determine Phosphoenolpyruvate Carboxykinase (PEPCK), Glucose-6-Phosphatase (Glc-6-Pase), Aspartate amino Transferase (AST) and Alanine Aminotransferase (ALT) enzymes. Moderate mean values of liver tissue PEPCK, Glc-6-Pase, AST and ALT were 562.00±7.25 U g-1, 160.00±4.25 mU g-1, 3.6±0.02 U g-1 and 2.6±0.01 U g-1, respectively. The mean values were significantly (p≤0.05) higher during hot and cold environmental temperatures in comparison to moderate mean value in each case. The sex and age effects were significant (p≤0.05) in all the environmental temperatures for all the four enzymes and pattern of variation was similar. The mean values were significantly (p≤0.05) higher in female animals than male animals. Age effect showed a significant (p≤0.05) increase in the mean values being highest in the animals of 2-4.5 years of age. Variation in the activities of all the enzymes studied clearly indicated the greater effect of hot environmental temperature on the modulation of gluconeogenesis. Hot period related change was maximum in the activity of PEPCK (4.29 fold) followed by Glc-6-Pase (3.5 fold), ALT (2.92 fold) and AST (2.38 fold). PEPCK and Glc-6-Pase are important enzymes of gluconeogenesis. Enhanced activities of these two enzymes showed adaptive response to combat the stress of environmental temperature through increased glucose production.
  R. Maan , N. Kataria , S. Sharma , S. Arora , A. Sharma , A. Joshi , N. Mohammad , P.K. Pilania and L.N. Sankhala
  Serum Monoamine Oxidase (MAO) activities were determined in the Marwari breed of sheep from arid tracts in India during extreme ambient temperature periods. Animals were categorised according to sex and age. Blood samples were collected during moderate, extreme hot and cold ambient temperatures to harvest sera. The overall mean value of MAO was 288.88±5.10 U L-1 which was obtained from 630 animals irrespective of sex and age. The range was 102-600 U L-1. Further mean values were determined according to ambient temperatures irrespective of sex and age. Moderate overall mean value was 162.14±4.61 U L-1 which was obtained from 210 animals during moderate ambience. The mean value of serum MAO was significantly (p≤0.05) higher during hot and cold ambient temperatures as compared to moderate mean value. The sex and age effects were significant (p≤0.05) in all ambient temperatures. The mean values were significantly (p≤0.05) higher in male animals than female animals. Age effect showed a significant (p≤0.05) increase in the mean values being highest in the animals of 2.5-4.5 years of age. The sex and age effects were significant (p≤0.05) in all ambient temperatures. Pattern of variations in serum MAO activities indicated that oxidative stress developed in sheep due to extreme ambient temperatures. Impact of hot ambient temperature was greater than cold ambient temperature.
  P.K. Pilania , S. Solanki , N. Mohammed , S. Asopa , R. Maan , A. Joshi , L.N. Sankhala , M. Mathur , M.K. Thori , J.S. Gaur , A. Meena and N. Kataria
  An investigation was carried out to assess oxidative stress in goats of Marwari breed affected from gastrointestinal parasitism and pneumonia belonging to farmers’ stock of arid tract of Rajasthan State, India. The animals were grouped into healthy and affected. All the blood samples were collected in sterile tubes with anticoagulants for erythrocyte separation and determination of erythrocytic Catalase (CAT) and Superoxide Dismutase (SOD) as biomarkers of oxidative stress. Activities of both the enzymes were significantly (p≤0.05) higher in affected animals in comparison to healthy lot. Erythrocytic catalase activity was 2.37 times higher in goats having gastrointestinal parasitism and 3.06 times higher in goats affected with pneumonia. Erythrocytic superoxide dismutase activity was 4.77 times higher in goats having gastrointestinal parasitism and 6.40 times higher in goats affected with pneumonia. It was observed that goats affected with pneumonia showed higher magnitude of enzyme activities in comparison to those having gastrointestinal parasites. Magnitude of change was greater in SOD activities than CAT activities. It can be concluded that gastrointestinal parasites and pneumonia served to produce oxidative stress in the goat. Catalase and superoxide dismutase are considered as potent biomarkers of oxidative stress. They are also named as antioxidant enzymes. Increased activities of both these enzymes signified the presence of oxidative stress in order to combat excessive production of free radicals. Antioxidant supplementation is recommended in cases affected with conditions like parasites and pneumonia to protect the animals from oxidative stress and ensuing damage to cellular machinery.
  N. Mohammed , S. Solanki , T. Bhati , P. Nathawat , S.K. Sharma , S. Kumar , A. Joshi , P.K. Pilania , L.N. Sankhala , M. Mathur , A. Ahuja , N. Kataria and A.K. Kataria
  The present study was carried out on 17 positive cases (horses) of colic. Two cases died during the treatment. Ten apparently healthy equines (horses) were also included in the study which constituted control group. Clinico-physiological observations included clinical signs, temperature, pulse, respiratory rate per min and colour of mucosa and were noted daily before start of treatment. Laboratory diagnosis of equine colic was done by determining haematological parameters. Blood samples from control group as well as equines suffering from colic were subjected to haematological examinations comprising of haemoglobin, packed cell volume, total erythrocyte count, total leucocyte count, differential leucocyte count and platelet count. There was great variation in duration and severity of clinical manifestations. In all the cases abdominal pain, loss of appetite, depression and change in colour of mucous membrane from light pink to dark pink was present. Pulse rate, respiration rate and capillary refill time were increased highly significantly (p<0.01) than the mean values from control group but temperature was within normal range and non-significantly (p>0.05) changed. In present study haemoglobin, PCV and TEC counts was increased significantly (p<0.05) on 1st day (before treatment) in comparison to healthy control group and after-treatment group. There was non significant (p>0.05) difference in their values between control group and after treatment group. Pattern of variation observed in the present study regarding haematological parameters of colicky cases can contribute in generating data for future studies and in laboratory diagnosis.
 
 
 
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