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Articles by L.A. Ogunkanmi
Total Records ( 3 ) for L.A. Ogunkanmi
  L.A. Ogunkanmi , W.O. Okunowo , O.O. Oyelakin , B.O. Oboh , O.O. Adesina , K.O. Adekoya and O.T. Ogundipe
  The genus Corchorus is one of the most important fibre crops, in order to improve this largely neglected crop, it is essential to understand the pattern of diversity in this important crop species. In this study, inter and intra genetic diversity analysis of 40 genotypes from C. olitorius and 40 genotypes from C. incisifolus was carried out using sixteen RAPD markers. The molecular study of the two species classified C. olitorius into two main clusters with five genotypes unresolved while there are three clusters with all the genotypes clearly separated in C. incisifolus. The results of both inter and intra genetic diversities revealed a higher level of allelic diversity in C. incisifolus than in C. olitorius. In the morphological study, higher mean and higher upper range values for all the five quantitative traits in C. incisifolus indicated its superiority over C. olitorius. However, both species show divergent attributes in four qualitative traits but similar in two characters. This could be attributed to higher level of natural outcrossing in C. incisifolus relative to that of C. olitorius. Results of this study therefore show that RAPD marker is a useful tool for assessing inter and intra genetic diversity in the two species of Corchorus. Therefore selection could be made from the diverse genotypes as parents for crosses designed for breeding improved jute cultivars and for producing mapping populations for QTL analysis.
  A.E. Tony-Odigie , K.O. Adekoya , S.C.O. Makinde , B.O. Oboh , L.A. Ogunkanmi and M.A. Fowora
  The genus, Amaranthus, is a typical annual flowering plant valued as vegetables, grains and ornamentals. It exhibits a high degree of interspecies variability and as such enhances biodiversity. Two common grain types (A. caudatus and A. cruentus) and three major weedy types (A. hybridus, A. spinosus and A. viridis) were studied. Phenotypic traits were determined using FAO descriptors for both qualitative and quantitative traits. Extracted DNA samples were amplified through the PCR technique using four RAPD primers (OPA-02, OPA-04, OPB-08 and OPE-01). Statistical analyses for morphological and molecular data were done using the NTSYS Version 2.02j software. Qualitative morphological characters did not significantly discriminate among the five species except for floral morphology. Quantitative characters, however, exhibited wide interspecies variation. Morphological cluster analysis showed that the five species were entirely distinct with a similarity coefficient of 0 except for A. cruentus and A. hybridus which shared an even very low coefficient of 0.093. The RAPD primers generated a total of 150 bands in the size range of 250-2000 bp. Molecular cluster analysis showed that all the species studied shared a similarity coefficient of 0.57 and some individuals within each species were clustered with individuals from other species. For example, most of the randomly selected plants from A. viridis were clustered with plants selected from A. spinosus at a similarity coefficient up to 0.81. This close relationship between A. viridis and A. spinosus may be of medicinal importance for both humans and animals especially since A. viridis is more appealing for consumption. The use of RAPD molecular marker systems in Amaranthus spp. should be advanced so as to impel specific linkage among genes controlling important traits.
  M.O. Sifau , L.A. Ogunkanmi , K.O. Adekoya , B.O. Oboh and O.T. Ogundipe
  The common name “Eggplant” is given to vegetables of several Solanum L. species that are important for human diet and health. The taxonomy and phylogenetic relationships among these taxa are currently unclear due to its large size and tropical center of diversity. This study assessed the genetic diversity in forty nine eggplant and related Solanum species’ genetic resources in Southern Nigeria using one nuclear (ITS) and two chloroplast genes. Analysis of DNA sequence data from the three regions (ITS, trnl C-trnl D and trnl E-trnl F) gave a high level of genetic variability (polymorphism) among the samples studied. A bootstrap value of 100 was observed between S. macrocarpon L. and S. torvum Sw. and between S. macranthum A. Rich. and S. indicum L., closely followed by a value of 99 between S. aethiopicum L. and S. dasyphyllum Schum. and Thonn. All these are indications of a close relationship between these species and a possibility of a common ancestor is strongly proposed. Solanum torvum was often separated out from S. melongena and even where they grouped together they have a low bootstrap value of 3 which is an indication of distant relatedness. The high level of intra and inter specific variations displayed within eggplant accessions and between its relatives as reported in this study could be effectively used in genetic improvement of cultivated eggplant varieties as well as in situ and ex situ conservation.
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