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Articles by L. Ramkumar
Total Records ( 3 ) for L. Ramkumar
  L. Ramkumar , T. Ramanathan , P. Thirunavukkarasu and N. Arivuselvan
  This study revealed that antioxidant properties, 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity and reducing power were observed in different mushroom species namely Agaricus bisporus, Pleurotus sajor-caju, Pleurotus euos, Hybsizus ulmarius, Pleurotus florida, Volveriela volvaciea, Pleurotus platypus, Pleurotus djamor and Calocybe indica. Methonolic extract of P. euos was recorded maximum levels of DPPH free radical scavenging (6 mg mL-1), reducing power (6 mg mL-1) and enzymatic antioxidants viz., catalase, superoxide dismutase and peroxidise, 68.7±1.1, 0.39±0.01, 42.21±0.06, 37.12±0.04 and 7.21±0.05, respectively. The maximum amount of enzymatic antioxidants were recorded viz., CAT, SOD and POX; 42.21±0.06, 37.12±0.04 and 7.21±0.05 μmol, respectively.
  P. Thirunavukkarasu , T. Ramanathan , N. Ravichandran and L. Ramkumar
  The objective of this study was to determine the potential anti-microbial activity of aqueous ethanolic and chloroform extracts from 3 medicinal plants against bacteria and some fungal strains. The antimicrobial efficacy of Citrullus colocynthis, Citrullus lanatus and Citrullus vulgaris, was evaluated against Gram negative, positive and some fungal strains and including Esherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Bacillus subtilis and Bacillus pumilus some fungal Candida albicans, Aspergillus niger, Penicillium chrysogenum and Penicillium chrysogenum using agar well diffusion method at concentration levels (100 mg mL-1). Among the tested plants, Citrullus colonthis extract showed the highest antibacterial and anti fungal activity and all bacterial and fungal strains at 100 mg mL-1. In addition, almost all species of plants were found to have activity on at least two microbial strains. However, the diameters of inhibition zone were different according to the kinds and concentrations of plant extracts and bacterial and fungal strains. The ethanol extract of citrullus colocynthis was very active against bacterial and fungal at a concentration of 100 mg mL-1. But Trichosporon begelli showed that no zone inhibition in C. valgaries plants of ethanol and chloroform extracts.
  P. Thirunavukkarasu , T. Ramanathan and L. Ramkumar
  Acanthus ilicifolius is a very potential plant and it has more phyto chemical compounds. In present study, anti microbial and hemolytic effects of Acanthus ilicifolius leaves extracts of chloroform and aqueous in isolated protein. The antimicrobial activity of crude extract against bacterial and fungal pathogens showed the clear inhibition zone against Vibrio cholerae and Aspergillus niger in chloroform extract and aqueous extracts showed clear inhibition zone for Pseudomonas sp. and Candida albicans. Both the extracts exhibited hemolytic activity which was estimated as 10.80 ht mg-1 for chloroform extract and 9.5 ht mg-1 for aqueous extract. The partial purification of protein is done by using DEAE cellulose. On SDS-PAGE the crude protein yielded three well defined bands at 100.5, 52 and 21.4 kDa in both the extracts.
 
 
 
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