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Articles by L. Naraghi
Total Records ( 2 ) for L. Naraghi
  L. Naraghi , A. Heydari and F. Azaddisfani
  In this study, Talaromyces flavus, a fungal antagonist, was isolated from soil samples that were collected from cotton fields in Golestan province of Iran. Antagonistic effects of non-volatile extracts of T. flavus isolates on Verticillium dahliae growth were investigated separately in laboratory experiments. Different isolates of T. flavus caused variable percents of growth inhibition of V. dahliae. Among isolates, ten of them that caused more growth inhibition of V. dahliae than other isolates, were selected for greenhouse experiments. In this step, first inoculum of microsclerotia of V. dahliae and seed treatments affected by non-volatile extracts and ascospores suspension of ten isolates of T. flavus were prepared. For comparison of infection index in seed treatment, greenhouse experiment was performed as a completely randomized design with forty-three treatments and three replications. Results of disease evaluation showed that among seed treatments affected by ascospores suspension of T. flavus isolates, seed treatments of isolates TF-2, Tf-12 and TF-17 caused maximum decrease in Verticillium wilt index. However, among seed treatments affected by non-volatile extracts of T. flavus isolates, seed treatments of isolates TF-1, TF-17 and TF-13 resulted in maximum decrease in disease index.
  H. Jahanifar , A. Heydari , N. Hasanzadeh , H.R. Zamanizadeh , S. Rezaee and L. Naraghi
  In this research, antibiotic-resistant mutants of antagonistic bacteria to rifampicin (Rif) and nalidixic acid (Nal) were developed via spontaneous mutation and their effectiveness in controlling cotton seedling damping-off disease was compared with their wild types. Four wild types isolates belonged to Pseudomonas fluorescens and their mutants were tested against pre-and post emergence damping-off disease of cotton in greenhouse. The experiment was conducted in a completely randomized design with ten treatments and four replicates. A virulent isolate of Rhizoctonia solani, the causal agent of cotton seedling damping-off disease was used in the study and the effectiveness of bacterial isolates was evaluated based on the number of healthy seedlings 15, 30, 45 and 60 days after sowing. Results indicated that all Rif-Nal resistant mutants showed more effectiveness in disease control than their wild types 15 and 30 days after sowing. However, at days 45 and 60 after sowing, only three mutants were more effective than their wild types in controlling cotton seedling damping-off disease. Results of this study suggest that spontaneous mutation may affect and enhance the activity and performance of antagonistic bacteria.
 
 
 
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