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Articles by L. Kannan
Total Records ( 3 ) for L. Kannan
  G. Ananthan , P. Sampathkumar , P. Soundarapandian and L. Kannan
  Totally 156 species of marine phytoplankton were identified of which Diatoms formed the dominant group followed by Dinoflagellates, Blue Green and Greens. Phytoplankton population density and species diversity were high during the summer season and were invariably sparse during the monsoon season at both the stations. Species richness and evenness of phytoplankton showed an inverse relationship with the dominance index. Distribution of chlorophyll a concentration closely followed the phytoplankton population density and gross primary productivity. Species composition, population density, primary productivity and chlorophyll a concentration was more in the coast due to higher number of autochthonous coastal marine species in addition to allochthonous species from the estuary.
  Milan Choudhury , Maloy Kumar Sahu , K. Sivakumar , T. Thangaradjou and L. Kannan
  Actinomycetes and Histamine Producing Bacteria (HPB) were isolated and identified from the Indian mackerel fish, Rastrelliger kanagurta from three different parts viz., skin with muscle, gills and gut. Among the three parts, gill harboured the highest number of actinomycetes (2.9x105 cfu g-1) and HPB (2.47x105 cfu g-1) than the other parts. At the same time, the fish harboured more number of actinomycetes than HPB. During the present investigation, a total of 29 HPB strains were isolated and identified as Bacillus sp., Pseudomonas sp., Vibrio sp. and Aeromonas sp. Among them, Pseudomonas sp. contributed more (45%) followed by Vibrio sp. (22%), Bacillus sp. (20%) and Aeromonas sp. (13%) in all the three parts of fish. In the present investigation, 41 strains of actinomycetes also isolated and all belonged to the genus, Streptomyces. Out of these 41 strains, 32 strains showed inhibitory activity to one or more HPB at varying levels. Among them, 3 strains viz., ASP-2, ASP-11 and ASP-15 showed good inhibitory activity against all 4 HPB and these 3 actinomycete strains (ASP-2, ASP-11 and ASP-15) were tentatively identified as S. aureofasciculus (ASP-2), S. chattanoogenesis (ASP-11) and S. hawaiiensis (ASP-15). The study indicates that the actinomycetes could be used to control HPB.
  B. Gunalakshmi , Maloy Kumar Sahu , K. Sivakumar , T. Thangaradjou , S. Sudha and L. Kannan
  Actinomycetes (20 strains), isolated from the sediments of shrimp pond were examined for their lipase activity. Strain LE-11, which was tentatively identified as Streptomyces griseochromogenes, showed higher lipase activity and it was taken for further study. Effects of various physical and chemical factors such as pH, temperatures, sodium chloride concentrations, carbon and nitrogen compounds on the lipase activity of S. griseochromogenes were studied. It was found that at pH 7, temperature 55°C, 0.05% NaCl concentration, carbon compound mannitol and nitrogen compound L-phenelylanine, the enzyme activity was maximum. Protein content of the crude enzyme was 2.057 µl mLG-1. The crude protein and partially purified protein were run in the SDS-PAGE and a band was found on equal position; however molecular weight of the protein was not determined. The study indicated that S. griseochromogenes can effectively be used in large scale production of lipase enzyme for commercial purposes, after ascertaining the strain=s ability in large scale fermentations.
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